Fabrication of sucrose biosensor based on single mode planar optical waveguide using co-immobilized plant invertase and GOD

Bagal, Dipali; Vijayan, Anu; Aiyer, R. C.; Karekar, R.N.; Karve, Meena

doi:10.1016/j.bios.2007.01.008

Cited by 32 publications

(16 citation statements)

References 34 publications

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“…These results are similar to those reported by Parida et al [6]. Biological activity of INV after nanogold formation was confirmed by DNSA method [26][27][28][29][30].…”

Section: Invertase-mediated Nanogold Synthesis: Uv-visible Studiessupporting

confidence: 82%

“…The onion membranes (diameter, 6.0 mm) were thoroughly washed and then incubated with glutaraldehyde (0.01%) in acetate buffer (pH 4.5) at 4°C in the dark, for 1 h. Activated membranes were washed gently three times to remove excess glutaraldehyde and treated with a mixture of invertase (500 μL, 220 U mL −1 ) and bovine serum albumin (1 mL, 1 mg mL −1 ) at 4°C for 12 h under gentle stirring. The onion membranes with immobilized invertase were washed twice and tested for enzyme activity using the DNSA method with sucrose (2.5×10 −8 M) as substrate [26][27][28]. Invertase-immobilized onion membranes were then stored at 4°C until use.…”

Section: Invertase Assay and Immobilizationmentioning

confidence: 99%

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Invertase-nanogold clusters decorated plant membranes for fluorescence-based sucrose sensor

Bagal-Kestwal¹,

Kestwal²,

Chiang³

2016

Nano Online

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In the present study, invertase-mediated nanogold clusters were synthesized on onion membranes, and their application for sucrose biosensor fabrication was investigated. Transmission electron microscopy revealed free nanoparticles of various sizes (diameter~5 to 50 nm) along with clusters of nanogold (~95 to 200 nm) on the surface of inner epidermal membranes of onions (Allium cepa L.). Most of the polydispersed nanoparticles were spherical, although some were square shaped, triangular, hexagonal or rod-shaped. Ultraviolet-visible spectrophotometric observations showed the characteristic peak for nanoparticles decorated invertase-onion membrane at approximately 301 nm. When excited at 320 nm in the presence of sucrose, the membranes exhibited a photoemission peak at 348 nm. The fluorescence lifetime of this nanogold modified onion membrane was 6.20 ns, compared to 2.47 ns for invertase-onion membrane without nanogold. Therefore, a sucrose detection scheme comprised of an invertase/nanogold decorated onion membrane was successfully developed. This fluorescent nanogold-embedded onion membrane drop-test sensor exhibited wide acidic to neutral working pH range (4.0-7.0) with a response time 30 seconds (<1 min). The fabricated quenching-based probe had a low detection limit (2x10 −9 M) with a linear dynamic range of 2.25x10 −9 to 4.25x10 −8 M for sensing sucrose. A microplate designed with an enzyme-nanomaterial-based sensor platform exhibited a high compliance, with acceptable percentage error for the detection of sucrose in green tea samples in comparison to a traditional method. With some further, modifications, this fabricated enzyme-nanogold onion membrane sensor probe could be used to estimate glucose concentrations for a variety of analytical samples.

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“…These results are similar to those reported by Parida et al [6]. Biological activity of INV after nanogold formation was confirmed by DNSA method [26][27][28][29][30].…”

Section: Invertase-mediated Nanogold Synthesis: Uv-visible Studiessupporting

confidence: 82%

Section: Invertase Assay and Immobilizationmentioning

confidence: 99%

Invertase-nanogold clusters decorated plant membranes for fluorescence-based sucrose sensor

Bagal-Kestwal¹,

Kestwal²,

Chiang³

2016

Nano Online

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“…A sucrose biosensor was developed based on an agarose guar gum-based binding matrix with incorporated acid invertase and glucose oxidase [95]. This film with a thickness of 12 μm was attached to a single mode optical waveguide that probed the refractive index changes ascribed to the specific reaction of sucrose in the matrix.…”

Section: Biosensor Implementationsmentioning

confidence: 99%

Thin Hydrogel Films for Optical Biosensor Applications

et al. 2012

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Hydrogel materials consisting of water-swollen polymer networks exhibit a large number of specific properties highly attractive for a variety of optical biosensor applications. This properties profile embraces the aqueous swelling medium as the basis of biocompatibility, non-fouling behavior, and being not cell toxic, while providing high optical quality and transparency. The present review focuses on some of the most interesting aspects of surface-attached hydrogel films as active binding matrices in optical biosensors based on surface plasmon resonance and optical waveguide mode spectroscopy. In particular, the chemical nature, specific properties, and applications of such hydrogel surface architectures for highly sensitive affinity biosensors based on evanescent wave optics are discussed. The specific class of responsive hydrogel systems, which can change their physical state in response to externally applied stimuli, have found large interest as sophisticated materials that provide a complex behavior to hydrogel-based sensing devices.

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“…Therefore, this review article will limit itself to the described scope. Some novel technologies such as silicon photonic biosensors, that have made tremendous impact on such applications will only be briefly discussed, but have been reviewed in detail elsewhere [ 7 ].…”

Section: Introductionmentioning

confidence: 99%

Waveguide-Based Biosensors for Pathogen Detection

Mukundan

Anderson

Grace

et al. 2009

Sensors

169

128

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Optical phenomena such as fluorescence, phosphorescence, polarization, interference and non-linearity have been extensively used for biosensing applications. Optical waveguides (both planar and fiber-optic) are comprised of a material with high permittivity/high refractive index surrounded on all sides by materials with lower refractive indices, such as a substrate and the media to be sensed. This arrangement allows coupled light to propagate through the high refractive index waveguide by total internal reflection and generates an electromagnetic wave—the evanescent field—whose amplitude decreases exponentially as the distance from the surface increases. Excitation of fluorophores within the evanescent wave allows for sensitive detection while minimizing background fluorescence from complex, “dirty” biological samples. In this review, we will describe the basic principles, advantages and disadvantages of planar optical waveguide-based biodetection technologies. This discussion will include already commercialized technologies (e.g., Corning’s EPIC® Ô, SRU Biosystems’ BIND™, Zeptosense®, etc.) and new technologies that are under research and development. We will also review differing assay approaches for the detection of various biomolecules, as well as the thin-film coatings that are often required for waveguide functionalization and effective detection. Finally, we will discuss reverse-symmetry waveguides, resonant waveguide grating sensors and metal-clad leaky waveguides as alternative signal transducers in optical biosensing.

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Fabrication of sucrose biosensor based on single mode planar optical waveguide using co-immobilized plant invertase and GOD

Cited by 32 publications

References 34 publications

Invertase-nanogold clusters decorated plant membranes for fluorescence-based sucrose sensor

Invertase-nanogold clusters decorated plant membranes for fluorescence-based sucrose sensor

Thin Hydrogel Films for Optical Biosensor Applications

Waveguide-Based Biosensors for Pathogen Detection

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