Outer Membrane Vesicle Proteomics to Discover the Pathogenicity of Acinetobacter baumannii

Tiwari, Vishvanath

doi:10.4172/2153-0602.1000e116

Cited by 2 publications

(2 citation statements)

References 25 publications

(34 reference statements)

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“…In some species, chromosomal encoded β-lactamases can be secreted in outer membrane vesicles (OMVs), enabling extracellular β-lactam degradation (Ciofu et al, 2000 ). Such OMVs are actually secreted by all Gram-negative bacteria and have different biological functions including protection of the secreted cargo, long-distance transport of toxins and virulence factors, cell-cell communication, pathogenesis, antibiotic resistance and aiding in biofilm formation (Deatherage et al, 2009 ; Bonnington and Kuehn, 2013 ; Tiwari, 2014 ). Recently, Ax21 was identified in S. maltophilia as an important OMV-associated virulence factor (McCarthy et al, 2011 ; Ferrer-Navarro et al, 2013 ).…”

Section: Introductionmentioning

confidence: 99%

The effect of imipenem and diffusible signaling factors on the secretion of outer membrane vesicles and associated Ax21 proteins in Stenotrophomonas maltophilia

et al. 2015

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Outer membrane vesicles (OMVs) are small nanoscale structures that are secreted by bacteria and that can carry nucleic acids, proteins, and small metabolites. They can mediate intracellular communication and play a role in virulence. In this study, we show that treatment with the β-lactam antibiotic imipenem leads to a dramatic increase in the secretion of outer membrane vesicles in the nosocomial pathogen Stenotrophomonas maltophilia. Proteomic analysis of their protein content demonstrated that the OMVs contain the chromosomal encoded L1 metallo-β-lactamase and L2 serine-β-lactamase. Moreover, the secreted OMVs contain large amounts of two Ax21 homologs, i.e., outer membrane proteins known to be involved in virulence and biofilm formation. We show that OMV secretion and the levels of Ax21 in the OMVs are dependent on the quorum sensing diffusible signal system (DSF). More specific, we demonstrate that the S. maltophilia DSF cis-Δ2-11-methyl-dodecenoic acid and, to a lesser extent, the Burkholderia cenocepacia DSF cis-Δ2-dodecenoic acid, stimulate OMV secretion. By a targeted proteomic analysis, we confirmed that DSF-induced OMVs contain large amounts of the Ax21 homologs, but not the β-lactamases. This work illustrates that both quorum sensing and disturbance of the peptidoglycan biosynthesis provoke the release of OMVs and that OMV content is context dependent.

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Section: Introductionmentioning

confidence: 99%

The effect of imipenem and diffusible signaling factors on the secretion of outer membrane vesicles and associated Ax21 proteins in Stenotrophomonas maltophilia

et al. 2015

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“…Outer membrane vesicles are also known to possess chromosomal encoded β-lactamases which causes the degradation of extracellular β-lactam [123]. All Gram negative bacteria have been synthesized such as OM vesicles by which they facilitate biofilm formation and many biological functions namely virulence factors transportation and antibiotics resistance [60,124,125]. Bacterial mobility is highly conserved in all Xanthomonas, and S. maltophilia species.…”

Section: Significance Of Omv Proteomics In the Study Of Drug Resistanmentioning

confidence: 99%

Significances of OMV and Extracellular Vesicle Proteomics

Tiwari¹

2017

J Data Mining Genomics Proteomics

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Outer Membrane Vesicle (OMV) proteome has been involved into the pathogenesis of diseases and resistance of microorganisms against a number of antibiotics, mechanism of action of probiotics and host-pathogen interaction etc. We have enlightened the role played by extracellular vesicles proteomics in the pathogenesis of different diseases related to human. Isolation succeeded by purification of ample amount of OMV from biological samples, is one of the most important steps for further proteome related analysis. With the development of both labelled and label-free methods used in proteomics, significant progress has been made in previous years in membrane proteomics. Hence, it is important to review the biological significance of proteins found in the OMV fractions using membrane proteomics approach. We have also explained methods used for isolation, purification and quantification of OMV. In the present review, it can be concluded that proteomic study of outer membrane and extracellular vesicles has now gained priority for the detailed study of disease pathogenesis, drug resistance, vaccine development, cell signalling etc.

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Outer Membrane Vesicle Proteomics to Discover the Pathogenicity of Acinetobacter baumannii

Cited by 2 publications

References 25 publications

The effect of imipenem and diffusible signaling factors on the secretion of outer membrane vesicles and associated Ax21 proteins in Stenotrophomonas maltophilia

The effect of imipenem and diffusible signaling factors on the secretion of outer membrane vesicles and associated Ax21 proteins in Stenotrophomonas maltophilia

Significances of OMV and Extracellular Vesicle Proteomics

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