Outer Hair Cell Molecular Protein, Prestin, as a Serum Biomarker for Hearing Loss

Parham, Kourosh; Dyhrfjeld-Johnsen, Jonas

doi:10.1097/mao.0000000000001164

Cited by 48 publications

(57 citation statements)

References 18 publications

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“…Highly specialized inner ear functions are made possible by unique proteins which upon disruption of function or cellular injury may be released into circulation where they could provide useful information. Specifically, we have proposed otolin-1 2 and prestin 12 as a serological biomarkers and provided proof of concept in BPPV 2 and noise-induced hearing loss 13 , respectively. Paving the path to practical application of inner ear biomarkers, however, requires additional validation.…”

Section: Discussionmentioning

confidence: 98%

Age-Related Increase in Blood Levels of Otolin-1 in Humans

Tabtabai

Haynes²,

Kuchel³

et al. 2017

Otology &Amp; Neurotology

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Objective To test the hypothesis that age-related demineralization of otoconia will result in an age-related increase in blood levels of otoconia matrix protein, otolin-1. Study design Cross-sectional observational clinical trial. Setting Clinical research center. Patients Seventy-nine men and women ranging in age from 22 to 95 years old. Interventions Diagnostic. Main outcome measures Blood levels of otolin-1 in relation to age. Results Levels of otolin-1 of subjects divided into four age groups (1: 20–30 [n=20], 2: 50–65 [n=20], 3: 66–80 [n=20], 4: 81–95 [n=19] years old) demonstrated an increasing trend with age The difference between otolin levels of groups 2 and 3, as well as, (P=0.04) and 2 and 4 (P=0.031) were statistically significant, but there was no significant difference between the two oldest groups. Conclusions Otolin-1 blood levels are significantly higher in patients older than 65 years of age. This is consistent with previous scanning electron microscopy findings of age-related otoconia degeneration and increased prevalence of benign paroxysmal positional vertigo (BPPV) with age. Normative data provided here can serve as important reference values against which levels from BPPV patients can be compared to further evaluate otolin-1 as a circulatory biomarker for otoconia degeneration.

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Section: Discussionmentioning

confidence: 98%

Age-Related Increase in Blood Levels of Otolin-1 in Humans

Tabtabai

Haynes²,

Kuchel³

et al. 2017

Otology &Amp; Neurotology

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“…Indeed, 2 weeks after noise trauma when there are missing OHCs in the cochlea, we have demonstrated decreased prestin levels relative to controls. 5 We only evaluated cisplatin at 8 mg/kg, but varying doses of cisplatin would likely accelerate or decelerate the rate of OHC damage and thus, prestin levels relative to baseline based on the rate of OHC damage. Alternatively, prestin levels may have remained elevated above the baseline due to a temporary elevation of prestin gene expression in the remaining OHCs that maintains circulatory prestin levels near baseline.…”

Section: Discussionmentioning

confidence: 99%

“…2,3 We extended this concept to sensorineural hearing loss and noise-induced hearing loss through measurement of the outer-hair cell-specific protein, prestin, in circulation. 4,5 In order for the concept of otologic biomarkers to be adopted into clinical practice, validation in other experimental settings, such as ototoxicity, is necessary. In addition, it should be demonstrated that biomarker levels are sensitive to interventions aimed at ameliorating otologic damage.…”

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confidence: 99%

Prestin as an Otologic Biomarker of Cisplatin Ototoxicity in a Guinea Pig Model

Naples

Cox

Bonaiuto

et al. 2017

Otolaryngol.--head neck surg.

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Objective To evaluate (1) whether changes in serum prestin aid in early detection of cisplatin ototoxicity, (2) the role of diltiazem as an otoprotectant, and (3) whether prestin levels are sensitive to effects of diltiazem. Study Design Experimental animal study. Setting Translational research laboratory. Subjects Twenty female guinea pigs. Methods Two groups of 10 guinea pigs were used. The relationship between serum prestin levels and auditory brainstem response (ABR) thresholds was compared between the groups. All animals had baseline blood draws and ABR thresholds recorded prior to cisplatin administration. Intraperitoneal cisplatin bolus (8 mg/kg) was administered followed by 5 consecutive days of intratympanic (IT) diltiazem (2 mg/kg) or sham IT-saline injection. Serum prestin levels and ABR thresholds were measured at days 1, 2, 3, 7, and 14 postcisplatin. Results In sham, IT-saline-treated animals, mean prestin levels were elevated above baseline on days 1 to 7. The prestin levels were significantly elevated from baseline on day 1 ( P < .001), while significant ABR threshold elevations did not occur until day 2 ( P = .028) for click-evoked ABRs and day 3 ( P = .041) for tones. In diltiazem-treated animals, prestin levels were not elevated above baseline but ABR thresholds were elevated on days 1 to 3. However, the thresholds returned toward baseline on days 7 and 14. Conclusion Changes in serum prestin levels were detectable prior to shifts in ABR thresholds in a guinea pig cisplatin ototoxicity model. These changes did not occur in diltiazem-treated animals. Prestin may serve as a biomarker of cochlear injury that is sensitive to therapeutic interventions in cisplatin ototoxicity.

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“…Other factors, including prestin, anti-prestin autoantibodies, and coenzyme Q10, may be prognostic in patients with SSNHL. Prestin, an outer hair cell (OHC)-specific protein, has been reported to be a biochemical marker for early diagnosis of SNHL loss in an animal model of noise-induced hearing loss and cisplatin ototoxicity [ 58 , 59 ]. Prestin concentration in serum, as measured by ELISA, was reported to be significantly higher in ISSHL patients than in normal controls ( p < 0.001).…”

Section: Biomarkers In Ssnhlmentioning

confidence: 99%

Biomarkers Suggesting Favorable Prognostic Outcomes in Sudden Sensorineural Hearing Loss

Doo

Kim

et al. 2020

IJMS

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Sudden sensorineural hearing loss (SSNHL) is a medical emergency, making detailed examination to determine possible causes and early treatment important. However, etiological examinations in SSNHL do not always reveal a cause, and several factors have been found to affect treatment outcomes. Various studies are being performed to determine the prognosis and effects of treatment in patients who experience sudden hearing loss, and to identify biomarkers associated with this condition. Embase, PubMed, and the Cochrane database were searched using the key words SSNHL, prognostic, and biomarker. This search identified 4 articles in Embase, 28 articles in PubMed, and 36 in the Cochrane database. Of these 68 articles, 3 were duplicates and 37 were unrelated to the research topic. After excluding these articles, the remaining 28 articles were reviewed. Factors associated with SSNHL were divided into six categories: metabolic, hemostatic, inflammatory, immunologic, oxidative, and other factors. The associations between these factors with the occurrence of SSNHL and with patient prognosis were analyzed. Low monocyte counts, low neutrophil/lymphocyte ratio (NLR) and monocyte/high-density lipoproteins (HDL) cholesterol ratio (MHR), and low concentrations of fibrinogen, platelet glycoprotein (GP) IIIa, and TNF-α were found to be associated with good prognosis. However, these factors alone could not completely determine the onset of and recovery from SSNHL, suggesting the need for future basic and clinical studies.

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Outer Hair Cell Molecular Protein, Prestin, as a Serum Biomarker for Hearing Loss

Cited by 48 publications

References 18 publications

Age-Related Increase in Blood Levels of Otolin-1 in Humans

Age-Related Increase in Blood Levels of Otolin-1 in Humans

Prestin as an Otologic Biomarker of Cisplatin Ototoxicity in a Guinea Pig Model

Biomarkers Suggesting Favorable Prognostic Outcomes in Sudden Sensorineural Hearing Loss

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