Outcome of cryotransfer of embryos developed from vitrified oocytes: double vitrification has no impact on delivery rates

Cobo, Ana; Castelló, D; Vallejo, Begoña Iza; Albert, Carmela; Santos, J.M. De los; Remohı́, José

doi:10.1016/j.fertnstert.2013.01.106

Cited by 31 publications

(22 citation statements)

References 42 publications

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“…In contrast, the patients in groups with donor oocytes were all at their second transfer and may therefore have had poor prognosis and low implantation rate at FET. This observation is in line with that reported by other authors investigating cryo-transfer from cryopreserved oocytes or after a failed first transfer [ 20 , 21 ]. It should also be pointed out that this is the only study in the literature giving information about the cryo-transfer of embryos obtained from vitrified donor oocytes in a transnational egg donation program in Europe.…”

Section: Discussionsupporting

confidence: 93%

Testing the efficacy and efficiency of a single “universal warming protocol” for vitrified human embryos: prospective randomized controlled trial and retrospective longitudinal cohort study

Parmegiani

Beilby

Arnone

et al. 2018

J Assist Reprod Genet

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PurposeTo study the efficacy and efficiency of a “universal warming protocol” for vitrified human embryos, based on subsequent steps with 1 and 0.5 M concentration of extracellular cryoprotectant (ECCP).MethodTwo studies on patients undergoing fertility treatments via ICSI: a prospective randomized controlled trial (RCT) and a retrospective cohort study (CS). Setting: Private assisted reproductive (AR) center.RCT: duration 01/03/2017–01/10/2017; 315 embryos at blastocyst stage obtained from 169 patients. Each patient’s embryos were first randomized for vitrification with two different kits: Vitrification Kit (Kitazato, Japan) and Sage Vitrification Kit (Origio, Denmark). The embryos were randomly warmed with either Kitazato (K) or Sage (S) warming kits, specifically: group A (KK), group B (KS), group C (SK), and group D (SS). Primary outcome measure: survival rate (number of embryos surviving per number of embryos warmed). Secondary: implantation rate (number of embryos implanted per number of embryos transferred).CS: duration 01/01/2013–31/12/2015 embryos from patients’ own oocytes; 10/04/2015–31/07/2017 embryos from donors’ oocytes. A total of 1055 embryos vitrified at cleavage stage obtained from 631 warming cycles: 847 of these obtained from patients’ own oocytes, 208 egg-donation-derived embryos. Each patient’s embryos were vitrified and warmed in various combinations of three different vitrification/warming kits: Kitazato (K), Sage (S), or made in-house in our laboratory (H). Vitrification/warming kits from different manufacturers are routinely used in our AR center, and the warming procedures are randomly performed with any available kit on a “first-in-first-out” basis, irrespective of the kit used for vitrification. Group names: KK, KS, SK, SS, SH, HK, HS, HH (embryos from patients’ own oocytes); eKK, eKS, eSK, eSS (egg-donation-derived embryos).ResultsCryo-survival rates were comparable in all study groups.RCT. Group A 99.0% (96/97), group B 98.8% (83/84), group C 98.4% (61/62), and group D 98.6% (71/72).CS. Embryos from patients’ own oocytes: KK 96.4% (54/56), KS 100.0% (13/13), SK 98.8% (80/81), SS 97.2% (174/179), SH 97.6% (40/41), HK 95.2% (20/21), HS 99.5% (187/188), and HH 97.4% (261/268). Egg-donation-derived embryos: eKK 100.0% (91/91), eKS 98.4% (60/61), eSK 100.0% (26/26), and eSS 96.7 (29/30).Implantation was generally comparable in all study groups—exceptions were in CS: KS vs. SK (P = 0.049), SS (P = 0.012), HS (P = 0.010), HH (P = 0.025); and SH vs. SS (P = 0.042), HS (P = 0.035).ConclusionWorldwide, millions of embryos have been cryopreserved using different vitrification kits; these studies establish that it is possible to combine different kits for vitrification and warming using a universal warming protocol. This can optimize costs, simplify lab routines, and favor embryo exchange between IVF centers.RCT registration numberISRCTN12342851.

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Section: Discussionsupporting

confidence: 93%

Testing the efficacy and efficiency of a single “universal warming protocol” for vitrified human embryos: prospective randomized controlled trial and retrospective longitudinal cohort study

Parmegiani

Beilby

Arnone

et al. 2018

J Assist Reprod Genet

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“…Several authors reported improved LBR in fresh IVF-ET cycles with blastocyst in comparison to day 3 ET [29], while others still debate whether ET at the blastocyst stage is superior to the early cleavage stage [26]. Even less data are available about the ideal embryo developmental stage of ET in the cases of embryos obtained from vitrified donor oocytes [30].…”

Section: Discussionmentioning

confidence: 99%

‘Two countries-two labs’: the transnational gamete donation (TGD) programme to support egg donation

Coccia

Rizzello

Wakunga

et al. 2020

J Assist Reprod Genet

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Purpose To evaluate the effectiveness, efficiency, and safety of a transnational gamete donation (TGD) programme based on the shipping of vitrified donor oocytes. Methods A retro-prospective observational study was conducted in the Assisted Reproductive Technology Center of the University Hospital of Florence, Italy. The study population included 622 consecutive donor oocyte cycles. A mean number of 6 vitrified oocytes per couple were shipped from two Spanish biobanks. In the receiving centre, gametes were warmed and inseminated and the subsequent embryo transfer (ET) was performed. The main outcome measurement was LBR. Secondary outcomes included oocyte survival rate, ICSI damage rate, normal fertilization, cleavage, and implantation rate (IR) in both ‘fresh’ and cryotransfer cycles. Results A total number of 3536 mature oocytes were warmed with 81.4% surviving oocytes. 2PN oocytes were 1941 with an ICSI normal fertilization rate of 70.4% and a cleavage rate of 93.4%; 857 day-3 embryos were transferred in 498 women, 63 blastocysts in 44. Couples with at least one vitrified embryo were 181 (32.3%). IR was 25.1% and 33.1% for day-3 ET and blastocyst stage respectively. Crude pregnancy rate and LBR after the first ET were 35.5% and 27% correspondingly with a conservative cumulative LBR of 34% and an optimal LBR of 51.4%. Conclusion Imported vitrified donor oocytes retain their competence and are capable of resulting in ongoing pregnancies and healthy babies in a proportion comparable to other existing systems as egg donation with vitrification/warming in the same laboratory and transnational fresh oocyte donation.

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“…104 Moreover, a subsequent study reporting the outcomes of cryotransfers of vitrified-warmed embryos developed from vitrified-warmed oocytes revealed that even double vitrification did not restrict live birth rates. 105 Beyond safe cryostorage of supernumerary material, a proficient embryo cryopreservation protocol encourages enhanced embryo evaluation other than by the more frequently performed morphokinetic analysis, for example, by means of biopsy and subsequent preimplantation genetic testing or other profiling studies on embryo development and metabolism. 106 In fact, these time-consuming methods are applied with some difficulties on fresh material, if not completely precluded.…”

Section: Cleavage Stage Embryos and Blastocystsmentioning

confidence: 99%

Perspectives in Gamete and Embryo Cryopreservation

Rienzi¹,

Iussig²,

Dovere³

et al. 2018

Semin Reprod Med

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Cryopreserved gametes and embryos are a major feature of human-assisted reproduction and patient care services, accounting for an increasing number of births worldwide. Since the first success obtained using frozen human spermatozoa, cryopreservation technology has been successfully extended to include oocytes and embryos, in a variety of both medical and nonmedical indications. Over the years, the available procedures have become widely implemented and the increasing evidence of its efficacy has contributed to acceptance of the technology. Nevertheless, a gold standard protocol that would be universally shared by clinics has yet to be definitively established and, therefore, research into cryopreservation of gametes and embryos cannot be considered concluded. Moreover, much effort should be committed to the definition and resolution of safety issues, the establishment of automation, and investigations about the potentiality of immature germ cells or stem cells.

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Outcome of cryotransfer of embryos developed from vitrified oocytes: double vitrification has no impact on delivery rates

Cited by 31 publications

References 42 publications

Testing the efficacy and efficiency of a single “universal warming protocol” for vitrified human embryos: prospective randomized controlled trial and retrospective longitudinal cohort study

Testing the efficacy and efficiency of a single “universal warming protocol” for vitrified human embryos: prospective randomized controlled trial and retrospective longitudinal cohort study

‘Two countries-two labs’: the transnational gamete donation (TGD) programme to support egg donation

Perspectives in Gamete and Embryo Cryopreservation

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