Outcome of Burkholderia (Pseudomonas) cepacia colonisation in children with cystic fibrosis following a hospital outbreak.

Occurrence of Multiple Genomovars of Burkholderia cepacia in Cystic Fibrosis Patients and Proposal of Burkholderia multivorans sp. nov.

“…Other epidemic strains which caused multiple deaths among cystic fibrosis patients in the United Kingdom (the Newcastle and Manchester outbreaks [28], represented by strains LMG 16658 and LMG 16659, respectively) also belong to genomovar 111. However, another epidemic strain affecting mainly healthy children belongs to genomovar I1 (the Glasgow outbreak [42], represented by strain LMG 16660).…”

Burkholderia cepacia: medical, taxonomic and ecological issues

“…Clarification of the clinical relevance of B. cepacia is also thwarted by the fact that the available scientific evidence requires particularly careful analysis. There is an inclination to link bacterial transmissibility and virulence, and to categorise individual B. cepacia Thomassen et al [57] LiPuma et al [58] LiPuma et al [59] Anderson et al [60] Millar-Jones et al [61] Govan et al [62] Smith et al [63] Bingen et al [64] Corkill et al [65] Pegues et al [66] Johnson et al [25] LiPuma et al [67] Ryley et al [68] Sun et al [69] Revets et al [70] Whiteford et al [71] Pitt et al [46] Seminal strains as either transmissible and virulent, or nontransmissible and avirulent. There is no scientific justification for this view.…”

“…However, by the early 1990s, the availability of selective culture media [48] and awareness of the organism's cultural idiosyncrasies [56] indicated that regional variation in the prevalence of B. cepacia colonisation could not be explained simply by laboratory methodology. Furthermore, the development and use of bacterial fingerprinting techniques-including multilocus enzyme electrophoresis (MLEE), pyrolysis mass spectroscopy, PCRribotyping and pulsed-field gel electrophoresis (PFGE)-provided compelling evidence for personto-person spread of B. cepacia through nosocomial and social contacts (Table 2) [25, 37,46,[57][58][59][60][61][62][63][64][65][66][67][68][69][70][71][72][73][74][75] and, occasionally, in the absence of proven sputum colonisation [67]. Epidemiological data also provided scientific justification for the introduction of guidelines by national CF organisations to improve personal and hospital hygiene and, more controversially, for the implementation of segregation policies to limit contact between colonised and non-colonised individuals [76].…”

Growth on mannitol-rich media elicits a genome-wide transcriptional response in Burkholderia multivorans that impacts on multiple virulence traits in an exopolysaccharide-independent manner

“…multivorans have been associated with high morbidity and mortality (Whiteford et al, 1995), and both species have been associated with cepacia syndrome (Zahariadis et al, 2003;Jones et al, 2004;Shafiq et al, 2011). In addition to their recognition as CF pathogens, both B. multivorans and B. cenocepacia have also been responsible for outbreaks amongst hospitalized non-CF patients, often being associated with poor patient outcomes (Woods et al, 2004;Mann et al, 2010;Graindorge et al, 2010;Hanulik et al, 2013).…”