Burkholderia cepacia: medical, taxonomic and ecological issues

Govan, John R. W.; Hughes, J. E.; Vandamme, Peter

doi:10.1099/00222615-45-6-395

Cited by 298 publications

(288 citation statements)

References 102 publications

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“…Water reabsorption from the CF airway lumen outweighs limited Cl À secretion resulting in the airway secretions becoming viscid and clearance of secretions is impaired causing airway obstruction [1]. This is further compounded by colonisation of the airway with organisms such as Pseudomonas aeruginosa (P. aeruginosa) and Burkholderia cenocepacia (B. cenocepacia) which are associated with a poor prognosis [2,3].…”

Section: Introductionmentioning

confidence: 99%

Effect of cystic fibrosis exacerbations on neutrophil function

Brockbank

Downey

Elborn

et al. 2005

International Immunopharmacology

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In cystic fibrosis (CF), inflammation is caused by persistent bacterial infection from Pseudomonas aeruginosa and Burkholderia cenocepacia in the lung and is characterised by the persistent infiltration of massive numbers of neutrophils which leads to lung injury. The aim of this present study was to investigate the effects of CF exacerbations on the reactivity of peripheral blood neutrophils compared to data from a normal healthy control population. Peripheral blood neutrophils were isolated from control subjects and CF patients before and after an exacerbation of their lung disease. Isolated neutrophils were stimulated with N-formyl-methionyl-leucyl-phenylalanine (fMLP) and phorbol 12-myristate 13-acetate (PMA) and the rate of superoxide generation and elastase activity measured and compared with neutrophils from healthy age-matched controls. Neutrophils from CF patients spontaneously generated higher levels of superoxide after resolution of the exacerbation compared to control neutrophils. The stimulated generation of superoxide from control neutrophils was not significantly different from neutrophils isolated from CF patients either before or after resolution of the CF exacerbation. Neutrophils from CF patients spontaneously released more elastase than control neutrophils but released less elastase than control neutrophils in response to fMLP. The stimulated release of elastase from neutrophils was not significantly different before compared to after resolution of the exacerbation. Neutrophils from CF patients displayed a different pattern of response than those from control subjects; however, CF exacerbations did not appear to modulate neutrophil function. D

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Section: Introductionmentioning

confidence: 99%

Effect of cystic fibrosis exacerbations on neutrophil function

Brockbank

Downey

Elborn

et al. 2005

International Immunopharmacology

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“…Despite the progress in taxonomy and a better understanding of the evolving nature of B. cepacia pulmonary infection in patients with CF, very little is known about bacterial features that contribute to pulmonary pathogenicity [7].…”

Section: Introductionmentioning

confidence: 99%

Invasion of murine respiratory epithelial cells in vivo by Burkholderia cepacia

Ostry

2001

Journal of Medical Microbiology

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Pulmonary infections caused by Burkholderia cepacia are an important cause of morbidity and mortality in cystic ®brosis (CF) patients. Several features suggestive of invasion and intracellular sequestration of B. cepacia in CF are persistence of infection in the face of antibiotic therapy and a propensity to cause bacteraemic infections in patients with CF. A mouse respiratory challenge model was used to investigate the invasion phenotype of B. cepacia in vivo. After intratracheal inoculation, epidemic B. cepacia strains translocated from lung to liver and spleen; however, all bacteria were cleared from all organs within 7 days. B. cepacia strains, irrespective of cable piliation, were capable of attaching to and then invading murine respiratory tract epithelial cells. Histopathological examination of lungs showed interstitial in®ltrates comprised mainly of polymorphonuclear leucocytes and were associated with widened alveolar septa. Electron microscopy demonstrated B. cepacia within epithelial cells and pulmonary macrophages. This study provides support for in-vitro observations that B. cepacia strains from patients with CF adhere to and then invade respiratory epithelial cells. The invasion phenotype in B. cepacia may be an important virulence factor in CF infections.

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“…Burkholderia cepacia, formerly known as Pseudomonas cepacia, is a gram-negative bacillus commonly found in soil, vegetation and water. It has emerged as an important opportunistic pathogen in immune compromised patients (Govan et al, 1996;Holmes et al, 1999) …”

Section: Resultsmentioning

confidence: 99%

Occurrence of Potentially Pathogenic Vibrio and related species in Seafoods obtained from the Eastern Province of Saudi Arabia

Ibrahim¹,

Shabeeb²,

Noureldin³

et al. 2016

Int. J. Adv. Res. Biol. Sci

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Seafoods are liable to bacterial contamination and could cause health risk to consumers. The present work is aimed to study the occurrence of potentially pathogenic and related species in seafoods obtained from the Eastern Province of Saudi Arabia. Studies were carried out to isolate, identify and characterized different bacterial species in 448 seafood samples comprising Fishes (353), Prawns (49) and Cuttlefishes (46), collected during the period from January 2015 until May 2016. TCBS agar was used for isolation and presumptive colonies both suspicious and non-suspicious for Vibrios were subcultured, characterized by biochemical tests and then identified at the genus and species level using API 20NE test kits. Species belongs to 8 bacterial families were isolated and the most predominant was Vibrionaceae 262 (58.4 %), followed by Aeromonadaceae 45 (10.4 %), Shewanellaceae 16 (3.57 %), Pasteurellaceae 13 (2.90 %), Caulobacteriaceae 9 (2.00 %), Pseudomonadaceae 7 (1.56 %), Enterobacteriaceae 7 (1.56 %) and Burkholderiaceae 6 (1.33 %). The presence of these organisms in the fresh seafood samples showed that the source of contamination may be from environment, catching, unhygienic handling and transportation. Thus it is strongly recommended that fresh seafoods must be properly stored at low temperature, good hygienic practices must be strictly followed and the seafoods must be adequately subjected to proper cooking before consumption.

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Burkholderia cepacia: medical, taxonomic and ecological issues

Cited by 298 publications

References 102 publications

Effect of cystic fibrosis exacerbations on neutrophil function

Effect of cystic fibrosis exacerbations on neutrophil function

Invasion of murine respiratory epithelial cells in vivo by Burkholderia cepacia

Occurrence of Potentially Pathogenic Vibrio and related species in Seafoods obtained from the Eastern Province of Saudi Arabia

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