Outbreak of Asthma Associated with Soybean Dust

Álvarez-Dardet, Carlos; Belda, José; Peña, Manuel; Nolasco, Andreu

doi:10.1056/nejm198910193211615

Cited by 83 publications

(1 citation statement)

References 9 publications

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“…[ 15 16 17 18 ] There are some reports suggesting that ET-1 is involved in the development of postischemic ARF in clinical transplantation. [ 19 20 ]…”

Section: Discussionmentioning

confidence: 99%

Preventive role of endothelin antagonist on kidney ischemia: Reperfusion injury in male and female rats

et al. 2015

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Background:Renal ischemia/reperfusion injury (RIRI) is the most common cause of acute kidney injury. We tested the protective role of endothelin-1 receptor blocker; bosentan (BOS) in animal model of RIRI in two different genders.Methods:Male and female Wistar rats were assigned as sham operated (sham), control group (ischemia), and case group (ischemia + BOS) treated with BOS (50 mg/kg) 2 h before bilateral kidney ischemia induced by clamping renal vessels for 45 min followed by 24 h of renal reperfusion.Results:The RIRI significantly increased the serum levels of blood urea nitrogen and creatinine in both genders (P < 0.05). These values were significantly decreased by BOS in both genders. In male rats, the serum levels of malondialdehyde in the ischemia + BOS group were decreased significantly when compared with ischemia group (P < 0.05).Conclusions:BOS can be used in both genders to attenuate kidney ischemia injury possibly due to its effect in the renal vascular system.

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“…[ 15 16 17 18 ] There are some reports suggesting that ET-1 is involved in the development of postischemic ARF in clinical transplantation. [ 19 20 ]…”

Section: Discussionmentioning

confidence: 99%

Preventive role of endothelin antagonist on kidney ischemia: Reperfusion injury in male and female rats

et al. 2015

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Assessment of Liver Allograft Function by Hyaluronic Acid and Endothelin Levels

Suehiro

Boros

Emre

et al. 1997

Journal of Surgical Research

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High-yield production of human big endothelin-1 by a combination of chemical modification and proteolysis of a fusion protein in Escherichia coli

Ôhashi

Yasufuku

Yano

1994

Appl Microbiol Biotechnol

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A protein modification method has been developed for the production of human big endothelin (ET)-1. Production of a large quantity of big ET-1 by the method described here is expected to facilitate future experiments such as X-ray crystallography and nuclear magnetic resonance studies, aimed at understanding the tertiary structure of big ET-1 and its dynamics. The plasmid pETB-50 used for the synthesis carries the gene for a fusion protein consisting of 34-amino acid (aa) residues of an N-terminal portion of beta-galactosidase and the 38-aa residues of big ET-1. The fusion protein ETB-50P contains an arginine residue in the big ET-1 portion at its second C-terminal site and three lysine residues including the C-terminal site in the beta-galactosidase portion, all of which are susceptible to trypsin. Tryptic digestion of the fusion protein quantitatively produced big ET-1 (1-37), which is depleted in the C-terminal serine. However, a treatment of the fusion protein with 1,2-cyclohexanedione prior to tryptic digestion gave full-length big ET-1 with N7, -N8-(1,2-dihydroxycyclohex-1,2- ylene)-arginine. This modification was reversed to the intact arginine residue when the modified big ET-1 was incubated in 0.5 M TRIS-HCl buffer, pH 8.0. Consequently, a combination of such a reversible protein modification and tryptic digestion gave 1.74 mg of recombinant big ET-1 from 2.01 of culture broth. The procedure described here may be applied to produce other arginine-containing peptides from fusion proteins.

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Outbreak of Asthma Associated with Soybean Dust

Cited by 83 publications

References 9 publications

Preventive role of endothelin antagonist on kidney ischemia: Reperfusion injury in male and female rats

Preventive role of endothelin antagonist on kidney ischemia: Reperfusion injury in male and female rats

Assessment of Liver Allograft Function by Hyaluronic Acid and Endothelin Levels

High-yield production of human big endothelin-1 by a combination of chemical modification and proteolysis of a fusion protein in Escherichia coli

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