Ostra niewydolność wątroby po podaniu amiodaronu

Wajtryt, Olga; Zielonka, Tadeusz M.

doi:10.5603/fc.2019.0056

Cited by 1 publication

(1 citation statement)

References 25 publications

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“…Acute liver failure (ALF) is caused by many factors, such as chronic use of medication (e.g., amiodarone, paracetamol), viral infections (e.g., hepatitis B virus), or diseases (e.g., Wilson’s disease) [ 1 , 2 ]. Despite the liver’s ability to self-regenerate from injuries induced by damaging factors, once the capacity for self-repair is exceeded, permanent and irreversible damage to the organ begins.…”

Section: Introductionmentioning

confidence: 99%

Comparison of the Efficacy of Two Routes of Administration of Human Amniotic Epithelial Cells in Cell Therapy of Acute Hepatic Insufficiency

Wieczorek,

Czekaj,

Król

et al. 2024

Pharmaceuticals

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The route of administration of implanted cells may affect the outcome of cell therapy by directing cell migration to the damaged site. However, the question of the relationship between the route of administration, the efficacy of colonisation of a given organ, and the efficacy of cell therapy has not been resolved. The aim of the study was to localise transplanted intravenously and intraperitoneally human amniotic epithelial cells (hAECs) in the tissues of mice, both healthy and injured, in an animal experimental model of acute liver failure (ALF). Mice intoxicated with D-Galactosamine (D-GalN) at a dose of 150 mg/100 g body weight received D-GalN alone or with a single dose of hAECs administered by different routes. Subsequently, at 6, 24, and 72 h after D-GaIN administration and at 3, 21, and 69 h after hAEC administration, lungs, spleen, liver, and blood were collected from recipient mice. The degree of liver damage and regeneration was assessed based on biochemical blood parameters, histopathological evaluation (H&E staining), and immunodetection of proliferating (Ki67+) and apoptotic (Casp+) cells. The biodistribution of the administered cells was based on immunohistochemistry and the identification of human DNA. It has been shown that after intravenous administration, in both healthy and intoxicated mice, most of the transplanted hAECs were found in the lungs, while after intraperitoneal administration, they were found in the liver. We concluded that a large number of hAECs implanted in the lungs following intravenous administration can exert a therapeutic effect on the damaged liver, while the regenerative effect of intraperitoneally injected hAECs on the liver was very limited due to the relatively lower efficiency of cell engraftment.

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Section: Introductionmentioning

confidence: 99%