Osthol and isopimpinellin from Fructus cnidii for the control of Dactylogyrus intermedius in Carassius auratus

Antifungal activity of lichen extracts and usnic acid for controlling the saprolegniasis

“…In our study, That acute toxicity to goldfish of usnic acid performed as described in literatures [15,16] with SC (4.152 mg L -1 )…”

The efficacy of four common anthelmintic drugs and traditional Chinese medicinal plant extracts to control Dactylogyrus vastator (Monogenea)

“…Each of the gill lamellas was placed on glass slide and the numbers of parasites on the gills were counted under a stereomicroscope to determine the mean number of parasites in each group. The anthelmintic efficacy of each treatment was calculated according to the following formula modified from Wang et al (2008): AE = [B − T] × 100% / B, where AE is anthelmintic efficacy, B is the mean number of surviving D. vastator in the control group and T is the mean number of surviving D. vastator in the treatment group.…”

“…This finding had since stimulated the re-screening of common drugs and extensive research to identify and develop alternative control strategies. In particular, this research has focused on the use of traditional medicinal plants as an alternative control strategy (Wang et al, 2008). Medicinal plants are considered to be more environmentally friendly and biodegradable than chemical anthelmintic agents (Rahuman et al, 2008).…”

Bioassay-guided isolation and identification of active compounds from Fructus Arctii against Dactylogyrus intermedius (Monogenea) in goldfish (Carassius auratus)

“…Fish were acclimatized under laboratory conditions for 7 days and cohabitated with the ones infested with D. intermedius which were cultured in our laboratory. The infected fish were prepared following the method described in our previous work (Wang et al 2008). Briefly, this procedure consisted of the collection of eggs, hatching of the eggs, and reinfection of D. intermedius.…”

“…The methanol extracts of Piper guineense seeds (Piperaceae) were effective to monogenean parasites (Ekanem et al 2004). Two compounds, osthol and isopimpinellin, from Frutus cnidii have been reported to have the anthelmintic efficacy against Dactylogyrus intermedius (Wang et al 2008).…”