Ostertagia ostertagi: Isolation and partial characterization of somatic and metabolic antigens☆

Canals, A.; Gasbarre, Louis C.

doi:10.1016/0020-7519(90)90048-r

Cited by 44 publications

(8 citation statements)

References 13 publications

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“…Serum reaction to non -separated parasite antigens using a double dif fusion technique, an ELISA-test or a dot blot technique might be easier but less specific methods than the western blotting. Similar applications of the immunoblotting technique were reported from immuno-parasitology by Clark et al (1988), Dickerson et al (1989) and Buchmann et al (1991), from veterinary science by Canals and Gasbarre (1990) and from human immuno-par asitology by Pritchard et al (1990). Further purification and cloning of the antigen in Oncho cerca volvulus were even reported by Lobos et al (1991) to create a high sensitive ELISA-method for detection of infected individuals.…”

Section: Serum Dilution 1: 200supporting

confidence: 56%

Immune Response of the Japanese Eel (Anguilla japonica) against Major Antigens from the Microsporean Pleistophora anguillarum Hoshina, 1951.

Buchmann¹,

Ogawa

1992

Fish Pathol.

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Section: Serum Dilution 1: 200supporting

confidence: 56%

Immune Response of the Japanese Eel (Anguilla japonica) against Major Antigens from the Microsporean Pleistophora anguillarum Hoshina, 1951.

Buchmann¹,

Ogawa

1992

Fish Pathol.

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“…In cattle, the most extensively studied GI nematode, in regards to host immune responses, is Ostertagia ostertagi. Within 3-4 weeks of experimental infection (Canals and Gasbarre, 1990;Mansour et al, 1990), and 2 months of exposure to infected pastures (Gronvold et al, 1992;Gasbarre et al, 1993;Nansen et al, 1993) previously naive calves show significant rises in anti-Ostertagia antibodies in the peripheral circulation. These antibody responses have been detectable using a wide range of parasite derived antigens, and involve all major immunoglobulin isotypes.…”

Section: Interaction Of Gi Nematodes and The Immune Systemmentioning

confidence: 99%

Role of the bovine immune system and genome in resistance to gastrointestinal nematodes

Gasbarre¹,

Leighton²,

Sonstegard³

2001

Veterinary Parasitology

119

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Gastrointestinal nematode infections of cattle remain a constraint on the efficient raising of cattle on pasture throughout the world. Most of the common genera of parasites found in cattle stimulate an effective level of protective immunity in most animals within the herd after the animals have been on pasture for several months. In contrast, cattle remain susceptible to infection by Ostertagia for many months, and immunity that actually reduces the development of newly acquired larvae is usually not evident until the animals are more than 2 years old. This prolonged susceptibility to reinfection is a major reason that this parasite remains the most economically important GI nematode in temperate regions of the world. Although, animals remain susceptible to reinfection for a prolonged period of time, there are a number of manifestations of the immune response that result in an enhanced level of herd immunity. These include a delay in the development time of the parasites, an increase in the number of larvae that undergo an inhibition in development, morphological changes in the worms, stunting of newly acquired worms, and most importantly a reduction in the number of eggs produced by the female worms. The overall result of these manifestations of immunity is a reduction in parasite transmission within the cattle herd. The immune mechanisms responsible for these different types of functional immunity remain to be defined. In general, GI nematode infections in mammals elicit very strong Th2-like responses characterized by high levels of Interleukin 4 (IL4), high levels of IgG1 and IgE antibodies, and large numbers of mast cells. In cattle, the most extensively studied GI nematode, in regards to host immune responses, is Ostertagia ostertagi. In Ostertagia infections, antigens are presented to the host in the draining lymph nodes very soon after infection, and within the first 3-4 days of infection these cells have left the nodes, entered the peripheral circulation, and have homed to tissues immediately surrounding the parasite where they become established. The immune response seen in the abomasum is in many ways are similar to that seen other mammalian hosts, with high levels of expression of IL4 in the draining lymph nodes and in lymphocytes isolated from the mucosa. But unlike a number of other systems, lymphocyte populations taken from Ostertagia infected cattle seem to be up-regulated for a number of other cytokines, most notably Interferon (IFN, implying that in Ostertagia infections, the immune response elicit is not simply a stereotypic Th2 response. In addition, effector cell populations in the tissues surrounding the parasites, are not typical, inferring the Ostertagia has evolved means to suppress or evade protective immune mechanisms. Studies have also demonstrated that the number of nematode eggs/gram (EPG) in feces of pastured cattle is strongly influenced by host genetics and that the heritability of this trait is approximately 0.30. In addition, EPG values are not "normally" distributed and a small...

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“…The first enzyme-linked immunosorbent assay (ELISA) test to quantify nematode infections in cattle was developed in 1981 (Keus et al), and since then, many in-house ELISAs have been developed for studies to quantify the infections and their related production losses (Kloosterman et al, 1984;Canals and Gasbarre, 1990;Dohoo et al, 1997;Eysker and Ploeger, 2000;Sanchez et al, 2001;Sithole et al, 2005b). More recently, Svanova (Svanova Veterinary Diagnostics, Uppsala, Sweden) developed a commercial ELISA (Svanovir ® ) available in Europe (Charlier et al, 2005a;Guiot et al, 2007;Forbes et al, 2008;Almería et al, 2009).…”

Section: Introductionmentioning

confidence: 99%

Predicting the effect of anthelmintic treatment on milk production of dairy cattle in Canada using an Ostertagia ostertagi ELISA from individual milk samples

Vanderstichel

Dohoo

Sánchez

et al. 2013

Preventive Veterinary Medicine

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Gastrointestinal nematodes, such as Ostertagia ostertagi and several species of Cooperia, are ubiquitous in temperate climates and have been shown to have detrimental effects on production in adult dairy cattle. A published meta-analysis demonstrated that overall, producers lose approximately 0.35 kg of milk per parasitized cow per day. Enzyme-linked immunosorbent assays (ELISAs) have the ability to quantify nematode infections in cattle, and thus, could be used to estimate the amount of milk production loss due to differing levels of parasitism at the individual cow level. 2ELISA results from individual cow milk samples were used to predict milk production response following a randomized anthelmintic treatment in a large field trial. To increase statistical power, the data collected from this field trial was pooled with data from two other published field trials to form an individual patient data meta-analysis (IPDMA).The ability to predict the effect of anthelmintic treatment on milk production depends on the level of parasitism quantified by an ELISA measuring milk antibodies against Ostertagia ostertagi, and reported as optical density ratios (ODRs). Therefore, the estimates from the interaction between ODR and treatment on milk production were used to determine how well the ODR predicted the response of the treatment. It was anticipated that the relationship between milk production and ODR was unlikely to be linear, so fractional polynomials were applied to the continuous ODR values.The interaction in the field trial showed a trend (p=0.138) towards a beneficial treatment effect when the individual ODR values, measured in late lactation and using Svanovir®, were greater than 0.12. When individual data from two other similar studies were included in an IPDMA, the interaction terms became statistically significant (p=0.009) indicating that there is a beneficial treatment effect when ODR values are slightly elevated. A graph was used to demonstrate the treatment effect (the estimated difference of kg/cow/day of milk yield between the treated and placebo cows), with 95% confidence intervals, as the ODR values increase. It is important to note that the methods of quantifying the ODR values differed between the three studies in the IPDMA, therefore some caution should be used when using these final estimated values. However, the shape and magnitude of the treatment 3 effects, as well as the other fixed model estimates, were very similar between the field trial and the IPDMA suggesting that any bias would likely be minimal.

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Ostertagia ostertagi: Isolation and partial characterization of somatic and metabolic antigens☆

Cited by 44 publications

References 13 publications

Immune Response of the Japanese Eel (Anguilla japonica) against Major Antigens from the Microsporean Pleistophora anguillarum Hoshina, 1951.

Immune Response of the Japanese Eel (Anguilla japonica) against Major Antigens from the Microsporean Pleistophora anguillarum Hoshina, 1951.

Role of the bovine immune system and genome in resistance to gastrointestinal nematodes

Predicting the effect of anthelmintic treatment on milk production of dairy cattle in Canada using an Ostertagia ostertagi ELISA from individual milk samples

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