Immune Response of the Japanese Eel (Anguilla japonica) against Major Antigens from the Microsporean Pleistophora anguillarum Hoshina, 1951.

Buchmann, Kurt; Ogawa, Kazuo; Lo, Chu Fang

doi:10.3147/jsfp.27.157

Cited by 5 publications

(4 citation statements)

References 10 publications

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“…From the spore itself, over 25 major protein bands could be distinguished, but only some of these proteins were immunogenic to the eel. This finding is consistent with an earlier report by Buchmann et al (1992). However, the present study shows for the first time that, as well as the serum antibodies, mucosal antibodies against P. anguillarum can also be found in Japanese eels.…”

Section: Discussionsupporting

confidence: 94%

“…However, the present study shows for the first time that, as well as the serum antibodies, mucosal antibodies against P. anguillarum can also be found in Japanese eels. Furthermore, in the present study, 10 protein bands wete recognized by immuno-blotting in the 54 naturally infected eels, whereas Buchmann et al (1992) observed only six distinct reaction bands in 16 infected Japanese eels. This discrepancy is perhaps accounted for by the greater number of eel sera samples in the present studies, which would naturally show a greater variability in the humoral immune response of eels against P anguillarum antigens.…”

Section: Discussioncontrasting

confidence: 60%

“…Nonetheless, the piscine acquired immune system has been reported to respond to parasites from various major protozoan groups including microsporideans (Leiro, Est^vez, Santamarina, Sanmartin & Ubeira 1993), and previous reports have shown that humoral immunity was produced by Japanese eel against /? anguillarum (Wang et al 1990;Buchmann, Ogawa & Lo 1992). The present paper describes further investigation of the humoral immune response of the Japanese eel to 7?…”

Section: Introductionmentioning

confidence: 83%

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Humoral immune response of Japanese eel, Anguilla japonica Temminck & Schlegel, to Pleistophora anguillarum Hoshina, 1951 (Microspora)

Hung

Tseng

et al. 1996

Journal of Fish Diseases

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A humoral response of Japanese eel, Anguilla japonica Temminck & Schlegel, to the microsporean Pleistophora anguillarum Hoshina was demonstrated using immunoblotting and an enzyme-linked immunosorbent assay (ELISA). Japanese eel immunoglobulin was purified by afifinity chromatography. The immunoglobulin was composed of 25-kDa light chains and 72-kDa heavy chains. The ELISA values of P. anguillarum antibodies in naturally infected fish sera were significantly higher than those of clinically healthy fish. Spore proteins from the microsporean were separated by electrophoresis and subjected to analysis by Western blot. Sera from naturally infected fish showed different reaction patterns to the spore proteins. While the sera randomly selected from naturally infected eels all showed a significant positive reaction to P anguillarum antigens, the mucus from only three out of the nine infected eels reacted positively in the ELISA test. Subsequent analyses indicated that there was no significant difference in the amount of mucus immunoglobufin among the tested eels. Therefore, the generally lower ELISA values of mucosal anti-/? anguillarum antibodies from the infected eels tested were evidently not caused by a lack of immunoglobulin per se, but seem to be the result of a lack of anti-7? anguillarum antibodies in the mucus and/or a lower afFmity in the anti-T? anguillarum antibodies that were present.

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Section: Discussionsupporting

confidence: 94%

Section: Discussioncontrasting

confidence: 60%

Section: Introductionmentioning

confidence: 83%

See 1 more Smart Citation

Humoral immune response of Japanese eel, Anguilla japonica Temminck & Schlegel, to Pleistophora anguillarum Hoshina, 1951 (Microspora)

Hung

Tseng

et al. 1996

Journal of Fish Diseases

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“…Humoral host responses against microsporidian parasites are known to occur. Japanese eel ( Anguilla japonica ) is able to mount an antibody reaction against a series of antigens in P. anguillarum (Buchmann et al 1992; Hung et al 1996) and the combined cellular and humoral reactions may be decisive elements when developing future immunoprophylactic strategies. Feeding of hosts with immunostimulants such as beta-glucans has been described to diminish the infection severity (Guselle et al 2010) but more effective methods should be considered.…”

Section: Microsporidiamentioning

confidence: 99%

Impact and control of protozoan parasites in maricultured fishes

Buchmann

2013

Parasitology

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Aquaculture, including both freshwater and marine production, has on a world scale exhibited one of the highest growth rates within animal protein production during recent decades and is expected to expand further at the same rate within the next 10 years. Control of diseases is one of the most prominent challenges if this production goal is to be reached. Apart from viral, bacterial, fungal and metazoan infections it has been documented that protozoan parasites affect health and welfare and thereby production of fish in marine aquaculture. Representatives within the main protozoan groups such as amoebae, dinoflagellates, kinetoplastid flagellates, diplomonadid flagellates, apicomplexans, microsporidians and ciliates have been shown to cause severe morbidity and mortality among farmed fish. Well studied examples are Neoparamoeba perurans, Amyloodinium ocellatum, Spironucleus salmonicida, Ichthyobodo necator, Cryptobia salmositica, Loma salmonae, Cryptocaryon irritans, Miamiensis avidus and Trichodina jadranica. The present report provides details on the parasites' biology and impact on productivity and evaluates tools for diagnosis, control and management. Special emphasis is placed on antiprotozoan immune responses in fish and a strategy for development of vaccines is presented.

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