Osteoporosis following chronic constriction injury of sciatic nerve in rats

Abstract

Osteopathic changes sometimes occur in patients with complex regional pain syndrome (reflex sympathetic dystrophy and causalgia). We aimed to investigate whether such osteopathic changes occurred in rats with chronic constriction injury (CCI) of the sciatic nerve. A CCI of the sciatic nerve was established in a unilateral hind limb in 39 adult Sprague-Dawley rats, which were killed 1, 2, 3, 5, or 7 weeks after the CCI procedure. Bone mineral content (BMC) and bone mineral density (BMD) in extracted tibial bones were measured using a dual-energy X-ray absorptiometer, and the number of osteoclasts in the metaphyseal regions was counted by the use of tartrate-resistant acid phosphate (TRAP) staining. BMC was significantly decreased, compared with that of the contralateral side, 1 to 7 weeks after CCI, and BMD was decreased 2 to 7 weeks after the procedure in the ipsilateral tibial bones, compared with BMD in the contralateral bones. The number of TRAP-positive multinucleated osteoclasts in the ipsilateral bones was significantly increased at 2, 3, and 5 weeks after the CCI, when compared with the number of these osteoclasts in the contralateral bones. The results of the present study demonstrate that osteopathic changes are associated with chronic constrictive injury of the sciatic nerve.

Effective Dosage and Administration Schedule of Oral Alendronate for Non-nociceptive Symptoms in Rats with Chronic Constriction Injury

“…Late treatment groups (HL, LL) were treated starting at the 14th day after CCI for 4 weeks. We chose the 14th day to categorize those into early (HE, LE) and late (HL, LL) groups based on results from Suyama et al (17) who showed that bone mineral density (BMD) significantly decreased from the second week after CCI.…”

Patchy Osteoporosis in Complex Regional Pain Syndrome

“…Some have postulated that immobilization plays a role in CRPS. Suyama et al, have (Suyama et al, 2002) observed a reduction in BMD 1 to 7 weeks postsurgery with an increase in the number of osteoclasts at 2, 3, and 5 weeks in their CRPS model. They have suggested that one possible mechanism would be the increase of bone resorption with immobilization.…”

“…Recovery of lost bone mineral content is slow and may persist after several years from the initial diagnosis (Nilsson, 1966) and this persistent regional osteoporosis can predispose to other future fractures after minor injuries (Sarangi et al, 1993). Same as the clinical manifestation, studies that have used rat models of CRPS have shown that bone mineral density significantly decrease from the second week (Suyama et al, 2002) and this loss is known to persist for at least 20 weeks .…”

The Effect of Sildenafil on Recuperation from Sciatic Nerve Injury in Rats

“…Jiang et al (38) reported that bone loss was 11.5% using dual-energy X-ray absorptiometry in rats with spinal cord injury on day 21. In another dual-energy X-ray absorptiometry study, bone loss was reported to be 8.4% in rats with chronic constriction injury (CCI) of the sciatic nerve at the fifth week (39). Cardozo et al (40) investigated whether nandrolone has protective effects against loss and slow muscle atrophy after nerve transection.…”