Osteopontin and Integrin αvβ3 Expression during the Implantation Window in IVF Patients with Elevated Serum Progesterone and Oestradiol Level

Abstract

!Background: To explore whether endometrial receptivity is determined by osteopontin (OPN) and integrin αvβ3 expression in women with elevated serum progesterone (P) and/or oestradiol (E 2 ) who are undergoing in vitro fertilisation (IVF). Methods: According to serum hormone levels on the day of HCG administration, 33 infertile women were divided into 3 groups: the high E 2 , high P, and high E 2 and P groups. The control group included 11 fertile, healthy women. Endometrial biopsy was performed on ovulation day + 7 to + 8 for all study participants, and the mRNA and protein expression levels of OPN and integrin αvβ3 were analyzed. Result: No statistically significant differences regarding OPN and integrin αvβ3 expression were found between infertile patients in the high P, high E 2 , high E 2 and P and control groups. There was no significant correlation between OPN and integrin αvβ3 staining intensity during the implantation window biopsy in any of the groups studied. Conclusion: Endometrial OPN and integrant αvβ3 expression/co-expression is not impaired during the window of implantation in patients with high P, high E 2 , or high E 2 and P levels. The clinical value of assessing endometrial receptivity with OPN and integrin αvβ3 seems to be uncertain.

A Novel Promising Endometrial Preparation Protocol for Frozen-Thawed Embryo Transfer: A Randomized Controlled Trial

“…The result of endometrial receptivity is embryo implantation, in return, embryo implantation rate reflects endometrial receptivity. Many studies and efforts have been made to improve endometrial receptivity but there is little consensus ( 21 , 22 ). However, it has been reported that CPR and IR increase when patients are given a period of artificial amenorrhea caused by GnRH-a ( 23 , 24 ).…”

Successful live birth in women with partial 17α-hydroxylase deficiency: report of two cases

“…Patients diagnosed with 17-OHD often suffer from infertility or related issues, due to the abnormal steroidogenic cascades cause impaired AND, 17-OHP and E 2 production together with progesterone and 11-deoxycorticosterone accumulation (19). The estradiol de ciencies and excessively high progesterone levels in these women results in abnormal endometrial maturation and follicular development (20,21). Weakened negative feedback of estrogen to the pituitary gland may lead to slightly elevated FSH and LH levels, predisposing patients to ovarian cysts, most of which are multiple luteinizing ovarian cysts, which may be caused by elevated serum progesterone or gonadotropins.…”

Transcriptome sequencing of endometrium revealed alterations in mRNAs and lncRNAs after ovarian stimulation

“…In the stimulation cycle, cell adhesion molecules (CAMs) and Wnt signaling pathways showed significant differences. Many members of the CAM family were previously shown to be associated with endometrial receptivity [16,17,[36][37][38]. The Wnt signaling pathway was also found to play an important role in embryo implantation [39].…”