Osteopontin: a marker for invasive oral squamous cell carcinoma but not for potentially malignant epithelial dysplasias

Routray, Samapika; Kheur, Supriya; Kheur, Mohit

doi:10.1016/j.anndiagpath.2013.03.005

Cited by 15 publications

(13 citation statements)

References 13 publications

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“…[21] This finding was supported by Routray et al ., who noted that the intensity of OPN expression increased significantly with loss of differentiation of tumor cells. [7] Contrary to this finding, our study showed a decrease in expression of OPN with increasing grades of OSCC suggesting a positive correlation between the expression of OPN protein and degree of differentiation. Further, the keratin pearls and surrounding oral epithelial cells in well- and moderately-differentiated SCCs showed positive OPN expression.…”

Section: Discussioncontrasting

confidence: 99%

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Immunolocalization of osteopontin in dysplasias and squamous cell carcinomas arising from oral epithelium

Aravind

Janardhanan

Rakesh

et al. 2017

J Oral Maxillofac Pathol

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Background:Early detection of oral squamous cell carcinoma (OSCC) remains one of the most efficient ways to ensure patient survival and improved quality of life. Although specific biomarkers related to OSCC have been investigated, a useful biomarker that assesses the transition potential of potentially malignant lesion to OSCC remains to be found. Osteopontin (OPN) has been recognized as an important factor in tumorigenesis and their expression in OSCC have been investigated earlier. In the present study, evaluation of OPN expression in premalignant and malignant lesions has been carried out to assess their possible role as a biomarker in the early diagnosis and prognosis of OSCC.Objectives:The objective of this study is to evaluate the role of OPN as a biomarker in the diagnosis and prognosis of OSCC.Materials and Methods:The study group consisted of archival paraffin-embedded blocks of ten cases each of varying grades of OSCC, oral epithelial dysplasias and epithelial hyperplasias. Sections were subjected to immunohistochemical staining for the biomarker OPN.Results:A positive OPN expression was noticed in epithelial dysplasias and SCC arising from the oral epithelium. A progressive increase in the intensity of staining was seen with increasing grades of dysplasias and a decrease in OPN expression with an increase in grades was observed in OSCC.Conclusion:The expression of OPN in full thickness of epithelium in severe dysplasias, carcinoma in situ, and in the superficial epithelium of OSCC suggest the possibility of considering OPN expression in full epithelial thickness in dysplasias as an indicator for malignant transformation.

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Section: Discussioncontrasting

confidence: 99%

“…The lack of expression in normal oral mucosa had been previously reported. [37] Staibano et al . noted that OPN expression was virtually undetectable in normal laryngeal mucosa and laryngeal hyperplasia.…”

Section: Discussionmentioning

confidence: 99%

Immunolocalization of osteopontin in dysplasias and squamous cell carcinomas arising from oral epithelium

Aravind

Janardhanan

Rakesh

et al. 2017

J Oral Maxillofac Pathol

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“…According to our study that reported by us earlier, the authors have concluded and highlighted OPN's role as a biomarker for malignancy in the form of invasion using IHC. [ 13 ]…”

Section: Discussionmentioning

confidence: 99%

Evaluating the efficacy of osteopontin expression as a prognostic marker in oral squamous cell carcinoma in the Indian subpopulation

Ingale¹,

Routray

Kheur

et al. 2014

J Oral Maxillofac Pathol

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Aim:This study aimed to correlate the prognostic value of osteopontin (OPN) expression using both tissue and plasma samples from patients with clinically and histologically confirmed oral squamous cell carcinoma (OSCC).Methods and Materials:The study group comprised of sixty patients (n = 60), which were clinically and histologically diagnosed for oral squamous cell carcinoma (OSCC). The Control group comprised of ten (n = 10) healthy volunteers. Plasma OPN levels were assayed using a quantitative enzyme-linked immunosorbent assay (OPN ELISA). Expression of OPN was also identified and evaluated by immunohistochemistry in tissue sections. These OPN expressions were then correlated with different parameters like age, sex, site, clinical presentation, tumor node metastasis (TNM) staging, histopathological grading and lymph node metastasis.Statistical Analysis:One-way analysis of variance (ANOVA) was used to evaluate the difference in tissue intensity and plasma OPN levels between the OSCC and the normal control groups.Results:The distribution of the plasma OPN levels and tissue OPN intensity in OSCC cohorts were compared to histopathological grades and analyzed. When evaluated OPN expression in tissue had higher intensity observed in OSCC (95% +ve) cases. And the mean plasma OPN concentration in OSCC cohort was more in comparison to the normal cohort. The results clearly showed that the plasma OPN levels and intensity grading in tissue correlated with tumor grades.Conclusion:The study highlights OPN as a biomarker for prognosis in OSCC in both plasma and tissue samples. We would like to emphasize on the evaluation of plasma OPN as a protocol of blood examination for all cancer patient, as it may serve as an indicator for tumor progression and potential risk of metastasis.

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“…The immunoreactivity was found in the bone matrix component of the regenerated tissues ( Figure 2, G-I ). 14 Human kidney tissue was used as an external positive control. As a negative control, the sections were submitted to the same procedure, with omission of the antibody.…”

Section: Immunohistochemistrymentioning

confidence: 99%

The influence of Aloe vera with mesenchymal stem cells from dental pulp on bone regeneration: characterization and treatment of non-critical defects of the tibia in rats

et al. 2019

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Objective This study aimed to evaluate the inflammatory effect and bone formation in sterile surgical failures after implantation of a collagen sponge with mesenchymal stem cells from human dental pulp (hDPSCs) and Aloe vera . Material and Methods Rattus norvegicus (n=75) were divided into five experimental groups according to treatment: G1) control (blood clot); G2) Hemospon ® ; G3) Hemospon ® in a culture medium enriched with 8% Aloe vera ; G4) Hemospon ® in a culture medium containing hDPSCs and G5) Hemospon ® in a culture medium enriched with 8% Aloe vera and hDPSCs. On days 7, 15 and 30, the animals were euthanized, and the tibia was dissected for histological, immunohistochemistry and immunofluorescence analyses. The results were analyzed using nonparametric Kruskal-Wallis test and Dunn’s post-test. Results On days 7 and 15, the groups with Aloe vera had less average acute inflammatory infiltrate compared to the control group and the group with Hemospon ® (p<0.05). No statistically significant difference was found between the groups regarding bone formation at the three experimental points in time. Osteopontin expression corroborated the intensity of bone formation. Fluorescence microscopy revealed positive labeling with Q-Tracker ® in hDPSCs before transplantation and tissue repair. Conclusion The results suggest that the combination of Hemospon ® , Aloe vera and hDPSCs is a form of clinical treatment for the repair of non-critical bone defects that reduces the inflammatory cascade’s effects.

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Osteopontin: a marker for invasive oral squamous cell carcinoma but not for potentially malignant epithelial dysplasias

Cited by 15 publications

References 13 publications

Immunolocalization of osteopontin in dysplasias and squamous cell carcinomas arising from oral epithelium

Immunolocalization of osteopontin in dysplasias and squamous cell carcinomas arising from oral epithelium

Evaluating the efficacy of osteopontin expression as a prognostic marker in oral squamous cell carcinoma in the Indian subpopulation

The influence of Aloe vera with mesenchymal stem cells from dental pulp on bone regeneration: characterization and treatment of non-critical defects of the tibia in rats

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