The influence of Aloe vera with mesenchymal stem cells from dental pulp on bone regeneration: characterization and treatment of non-critical defects of the tibia in rats

Abstract

Objective This study aimed to evaluate the inflammatory effect and bone formation in sterile surgical failures after implantation of a collagen sponge with mesenchymal stem cells from human dental pulp (hDPSCs) and Aloe vera . Material and Methods Rattus norvegicus (n=75) were divided into five experimental groups according to treatment: G1) control (blood clot); G2) Hemospon ® ; G3) Hemospon ® in a culture medium enriched with 8% Aloe vera ; G4) Hemospon ® in a culture medium containing hDPSCs and G5) Hemospon ® in a culture medium enriched with 8% Aloe vera and hDPSCs. On days 7, 15 and 30, the animals were euthanized, and the tibia was dissected for histological, immunohistochemistry and immunofluorescence analyses. The results were analyzed using nonparametric Kruskal-Wallis test and Dunn’s post-test. Results On days 7 and 15, the groups with Aloe vera had less average acute inflammatory infiltrate compared to the control group and the group with Hemospon ® (p<0.05). No statistically significant difference was found between the groups regarding bone formation at the three experimental points in time. Osteopontin expression corroborated the intensity of bone formation. Fluorescence microscopy revealed positive labeling with Q-Tracker ® in hDPSCs before transplantation and tissue repair. Conclusion The results suggest that the combination of Hemospon ® , Aloe vera and hDPSCs is a form of clinical treatment for the repair of non-critical bone defects that reduces the inflammatory cascade’s effects.

Renal Progenitor Cells Have Higher Genetic Stability and Lower Oxidative Stress than Mesenchymal Stem Cells during In Vitro Expansion

“…Nevertheless, MSCBMsu showed a logarithmic increase over nine days before showing a reduction in cell concentration ( Figure 1 ). This characteristic is not uncommon among mesenchymal stem cell lines, and studies with bone marrow, adipose tissue, and dental pulp lineages have commonly demonstrated similar evidence [ 33 – 35 ]. This observation raises the possibility that this group of somatic stem cells may present relevant metabolic adaptability.…”

Synthetic Scaffold/Dental Pulp Stem Cell (DPSC) Tissue Engineering Constructs for Bone Defect Treatment: An Animal Studies Literature Review

“…The histological evaluation was performed for a total of 28 of the selected papers (93.3%) [ 44 , 45 , 46 , 47 , 48 , 49 , 50 , 51 , 52 , 53 , 54 , 55 , 56 , 57 , 58 , 59 , 60 , 61 , 62 , 63 , 64 , 65 , 66 , 67 , 68 , 69 , 70 , 71 ] and histomorphometry was conducted in 25 of the studies’ experiments [ 45 , 46 , 47 , 48 , 49 , 50 , 51 , 52 , 53 , 54 , 55 , 56 , 57 , 58 , 60 , 62 , 63 , 64 , 65 , 66 , 67 , 68 , 69 , 70 , 71 ] (83.3%). For a total of 18 studies (60%) micro-CT evaluation was performed [ 44 , 45 , ...…”

“…A total of 19 articles were conducted on rat models and the DPSC culture passage ranged from one to six [ 44 , 45 , 46 , 47 , 48 , 49 , 50 , 51 , 52 , 53 , 54 , 55 , 56 , 57 , 58 , 59 , 60 , 61 , 62 ]. A total of 14 papers were conducted on rat calvaria/critical size defects [ 46 , 47 , 48 , 49 , 50 , 51 , 53 , 54 , 55 , 56 , 58 , 60 , 62 ], 2 on dental alveolar defects/maxillary [ 44 , 61 ], 2 on dental alveolar defects/mandibular and 1 article studied tibial bone defects [ 45 , 52 ].…”

“…A total of 14 papers were conducted on rat calvaria/critical size defects [ 46 , 47 , 48 , 49 , 50 , 51 , 53 , 54 , 55 , 56 , 58 , 60 , 62 ], 2 on dental alveolar defects/maxillary [ 44 , 61 ], 2 on dental alveolar defects/mandibular and 1 article studied tibial bone defects [ 45 , 52 ]. Therefore, many different bone scaffolds were used alone or in association with DPSCs, such as atelocollagen sponge [ 49 ], matrigel [ 44 , 48 ], puramatrix [ 45 ], dense collagen scaffold and gels [ 46 , 47 , 60 , 61 , 62 ], hemospon [ 57 ], inorganic bovine bone [ 54 , 59 ], 140 µm thick quartz membrane [ 48 ], β-tricalcium phosphate scaffolds (β-TCP) [ 52 , 56 ], composite strontium folate (SrFO)-TCP [ 55 ], hydroxiapatite/tricalcium phosphate paste (HA-TCP) [ 50 ], hydrogels [ 51 ], biodegradable polyesters [ 58 , 64 ], nanofibers [ 53 ].…”

Human Dental Pulp Stem Cells: recent findings and current research

“…Sparse cell conditions are more suitable for hard tissue regeneration to maintain the osteoblast and chondrocytes differentiation potential of DPSCs [20]. Isolated heterozygous cell population contain not only DPSCs, but also other types of cells that adhere to plastic, therefore only CD29, CD44, CD105 and STRO-1 positive cells are selected by fluorescence activating cell sorting (FACS), or alternatively magnetic activated cell sorting (MACS) [20,26]. Single Cell Raman Spectroscopy (SCRM) is the first tool to accurately discriminate and select for best quality DPSCs, improving the efficiency of stem cell therapy [27].…”