Osteoinductive potential of recombinant BMP-9 in bone defects of mice treated with antiresorptive agents

Fujioka‐Kobayashi, Masako; Marjanowski, S.D.; Kono, Michihide; Hino, Shunsuke; Saulačić, Nikola; Schaller, Benoît

doi:10.1016/j.ijom.2021.08.014

Cited by 3 publications

(4 citation statements)

References 24 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The binding of active molecules to the membrane is based on the hypothesis that exposure of the treated area to multiple different growth factors can trigger the development of a favorable microenvironment and promote bone regeneration [ 25 , 155 ]. Platelet-derived growth factor (PDGF) is a potent mitogenic and chemo-inductive agent, and in particular, PDGF-BB is more effective than other isoforms such as PDGF-AA and PDGF-AB in promoting mitosis in periodontal cells [ 156 ].…”

Section: Absorbable Barrier Membranementioning

confidence: 99%

“…Saulacic et al showed that in a rabbit cranial defect model, BMP-9 loaded on collagen membranes induced better horizontal bone defect closure than loading on deproteinized bovine bone mineral, and both combinations positively induced bone regeneration [ 77 , 167 ]. The significant bone-promoting potential of the combination of BMP-9 and collagen membranes has also been demonstrated in an anti-resorptive therapy (AMART) mouse model [ 25 ]. In other studies, stromal cell-derived factor-1α (SDF-1α) has been widely accepted to promote the recruitment, proliferation, and differentiation of bone marrow-derived stromal stem cells (BMSCs) as a classical chemokine, mediating significant bone regeneration and angiogenesis [ 129 , 168 ].…”

Section: Absorbable Barrier Membranementioning

confidence: 99%

“…A further focus for improvement of the performance of a resorbable barrier membrane is to have influence on their “bioactivity”, including factors such as transmembraneous vascularization or different approaches for “immunomodulating” properties such as influence on macrophage phenotypization [ 19 , 23 ]. In this context, an increasing number of preclinical studies are focusing on the loading of active compounds such as growth factors, cytokines, inorganic compounds, and anti-inflammatory agents among different others [ 3 , 24 , 25 ]. With the emphasis on membrane bioactivity, membrane-associated cellular and molecular events have gained much interest including cell recruitment, inflammation, and bone healing [ 26 , 27 ].…”

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

Barrier Membranes for Guided Bone Regeneration (GBR): A Focus on Recent Advances in Collagen Membranes

Ren

Fan

Alkildani³

et al. 2022

IJMS

View full text Add to dashboard Cite

Guided bone regeneration (GBR) has become a clinically standard modality for the treatment of localized jawbone defects. Barrier membranes play an important role in this process by preventing soft tissue invasion outgoing from the mucosa and creating an underlying space to support bone growth. Different membrane types provide different biological mechanisms due to their different origins, preparation methods and structures. Among them, collagen membranes have attracted great interest due to their excellent biological properties and desired bone regeneration results to non-absorbable membranes even without a second surgery for removal. This work provides a comparative summary of common barrier membranes used in GBR, focusing on recent advances in collagen membranes and their biological mechanisms. In conclusion, the review article highlights the biological and regenerative properties of currently available barrier membranes with a particular focus on bioresorbable collagen-based materials. In addition, the advantages and disadvantages of these biomaterials are highlighted, and possible improvements for future material developments are summarized.

show abstract

Section: Absorbable Barrier Membranementioning

confidence: 99%

Section: Absorbable Barrier Membranementioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Barrier Membranes for Guided Bone Regeneration (GBR): A Focus on Recent Advances in Collagen Membranes

Ren

Fan

Alkildani³

et al. 2022

IJMS

View full text Add to dashboard Cite

show abstract

“…Recombinant human BMP-9 is incubated at a concentration of 100 ng/mL onto bioceramic material after a soaking period in a shaking incubator at 37 °C [ 7 ]. The calvarial bone deficiencies were filled with a 3 mm diameter circle of collagen membrane and 500 ng of recombinant human BMP-9 [ 48 ]. In our study, doses of 0, 0.1, 1, 10, and 100 ng/mL were used, and it was difficult to determine the optimal concentration for osteogenic differentiation.…”

Section: Discussionmentioning

confidence: 99%

Bone Morphogenetic Protein-9 Promotes Osteogenic Differentiation and Mineralization in Human Stem-Cell-Derived Spheroids

Lee

Park

2023

Medicina

View full text Add to dashboard Cite

Background and Objectives: Alkaline phosphatase activity, mineralized matrix, and osteogenic-related gene expression have been shown to increase in response to bone morphogenetic protein-9 (BMP-9). In this study, spheroids derived from human gingival stem cells were used to determine the effects of BMP-9 on cell survival, osteogenesis, and mineralization. Materials and Methods: Human gingival stem cells were used to produce spheroids and then grown to concentrations of 0, 0.1, 1, 10, and 100 ng/mL with BMP-9. On days 1, 3, 5, and 7, morphological examination was carried out. A live/dead assay and Cell Counting Kit-8 was used to assess the vitality of cells. On days 7 and 14, alkaline phosphatase activity assays were carried out using a commercially available kit to examine the osteogenic differentiation of cell spheroids. Alizarin Red Staining was performed on the 7th and 14th days to evaluate mineralization, and RUNX2 and COL1A1 expression levels were evaluated on the 7th and 14th days using real-time polymerase chain reactions. Results: The BMP-9 added at the measured quantities did not appear to alter the shape of the well-formed spheroids produced by stem cells on day 1. In addition, treatment with BMP-9 at doses of 0, 0.1, 1, 10, or 100 ng/mL did not significantly alter cell diameter. Throughout the whole experimental process, viability was maintained. On day 14, the alkaline phosphatase activity in the groups dosed with 0.1, 1, 10, or 100 ng/mL was statistically higher than that in the unloaded control group (p < 0.05). According to qPCR data, the mRNA expression level of RUNX2 with 1 ng/mL dosing was higher on day 7 compared to that of the unloaded control group (p < 0.05). Conclusions: These findings suggest that BMP-9 can be employed to stimulate early osteogenic differentiation in stem cell spheroids.

show abstract

An insight of Osthole, Bone marrow mesenchymal stem cells, and BMP-9 loaded carbon-based scaffolds as a Biomaterial candidate in Osteoporosis Therapy: A Narrative Review

Bahtiar

Anwar

Farhana

et al. 2023

RJPT

View full text Add to dashboard Cite

Background: More than 200 million people worldwide diagnosed with osteoporosis, a degenerative condition characterized by decreasing bone mineral density. Although oral bisphosphonates are the most used form of treatment for osteoporosis, they have the potential to result in osteonecrosis. Osthole bone marrow mesenchymal stem cells (OBMMSCs) are combination of bone marrow mesenchymal stem cells (BMMSCs) and coumarin derivatives from Cnidium monnieri act as anti-inflammatory and anti-osteoporosis agents. The use of OBMMSCs in triad tissue engineering necessitates using a growth factor and a scaffold, which are combined with BMP-9 incorporated in carbon-based scaffold namely nano hydroxyapatite/collagen I/multi-walled carbon nanotubes (nHA/ColI/MWCNTs) scaffold to promote better loading. The combination of OBMMSCs and BMP-9 loaded nHA/ColI/MWCNTs scaffold has potential to increase OBMMSC differentiation into osteoblasts, resulting in increased bone remodeling and a better outcome in osteoporosis therapy. Purpose: To analyze the potential of OBMMSCs and BMP-9 loaded nHA/ColI/MWCNTs scaffolds as osteoporosis therapy. Discussion: Osthole enhances BMMSCs differentiation via activation of cAMP/CERK and Wnt/β-catenin/BMP signaling pathways. Osthole increases alkaline phosphatase (ALP) expression that stimulates osteogenesis. Meanwhile, in the Wnt/β-catenin-BMP pathway, osthole initiates Wnt binding to the Fz receptor so that β-catenin expression increases. β-catenin, together with Runx2, reduces the expression of GSK-3β, thereby increasing BMMSCs differentiation into osteoblasts. BMP-9 loaded nHA/COLI/MWCNTS scaffold promotes the differentiation of BMMSCs into osteoblasts by increasing the supply of collagen and calcium, as well as angiogenesis, which increases vascularity. Conclusion: OBMMSCs and BMP-9 loaded nHA/ColI/MWCNT scaffold have the potential to treat osteoporosis.

show abstract

Osteoinductive potential of recombinant BMP-9 in bone defects of mice treated with antiresorptive agents

Cited by 3 publications

References 24 publications

Barrier Membranes for Guided Bone Regeneration (GBR): A Focus on Recent Advances in Collagen Membranes

Barrier Membranes for Guided Bone Regeneration (GBR): A Focus on Recent Advances in Collagen Membranes

Bone Morphogenetic Protein-9 Promotes Osteogenic Differentiation and Mineralization in Human Stem-Cell-Derived Spheroids

An insight of Osthole, Bone marrow mesenchymal stem cells, and BMP-9 loaded carbon-based scaffolds as a Biomaterial candidate in Osteoporosis Therapy: A Narrative Review

Contact Info

Product

Resources

About