Osteogenic protein-1 with transforming growth factor-β1: potent inducer of chondrogenesis of synovial mesenchymal stem cells in vitro

Miyamoto, Chikara; Matsumoto, Tomoko; Sakimura, Koichiro; Shindo, Hiroyuki

doi:10.1007/s00776-007-1176-4

Cited by 41 publications

(30 citation statements)

References 29 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…This observation was confirmed by histological staining. Our findings are consistent with Miyamoto's report that BMP-7 in combination with TGF-b1 enhances chondrogenesis of synovial mesenchymal stem cells [Miyamoto et al, 2007]. Additionally, a published in vivo study demonstrated the regenerative capacity of BMP-7 in injured disc cells [Masuda et al, 2006], potentially due to the anti-apoptotic and anabolic activity [Stove et al, 2006] of BMP-7.…”

Section: Discussionsupporting

confidence: 93%

“…In spite of its main clinical use in spinal fusion and fracture non-unions [Giannoudis and Tzioupis, 2005], BMP-7 demonstrated synergism with microfractures to stimulate cartilage repair in vivo [Kuo et al, 2006]. BMP-7 can stimulate a chondrogenic phenotype in adipose tissue derived stem cells [Knippenberg et al, 2006] and enhances the chondrogenesis of synovial mesenchymal stem cells when combined with TGF-b1 [Miyamoto et al, 2007]. Interestingly, BMP-7 initiated adipogenic instead of osteo-/ chondrogenic differentiation of BM MSCs in high-density micromass cultures, which generally favor chondrogenic differentiation [Neumann et al, 2007].…”

mentioning

confidence: 96%

See 1 more Smart Citation

The role of BMP‐7 in chondrogenic and osteogenic differentiation of human bone marrow multipotent mesenchymal stromal cells in vitro

Shen

Wei

Whittaker

et al. 2009

J of Cellular Biochemistry

142

View full text Add to dashboard Cite

This study addresses the role of bone morphogenetic protein-7 (BMP-7) in chondrogenic and osteogenic differentiation of human bone marrow multipotent mesenchymal stromal cells (BM MSCs) in vitro. BM MSCs were expanded and differentiated in the presence or absence of BMP-7 in monolayer and three-dimensional cultures. After 3 days of stimulation, BMP-7 significantly inhibited MSC growth in expansion cultures. When supplemented in commonly used induction media for 7-21 days, BMP-7 facilitated both chondrogenic and osteogenic differentiation of MSCs. This was evident by specific gene and protein expression analyses using real-time PCR, Western blot, histological, and immunohistochemical staining. BMP-7 supplementation appeared to enhance upregulation of lineage-specific markers, such as type II and type IX collagens (COL2A1, COL9A1) in chondrogenic and secreted phosphoprotein 1 (SPP1), osteocalcin (BGLAP), and osterix (SP7) in osteogenic differentiation. BMP-7 in the presence of TGF-beta3 induced superior chondrocytic proteoglycan accumulation, type II collagen, and SOX9 protein expression in alginate and pellet cultures compared to either factor alone. BMP-7 increased alkaline phosphatase activity and dose-dependently accelerated calcium mineralization of osteogenic differentiated MSCs. The potential of BMP-7 to promote adipogenesis of MSCs was restricted under osteogenic conditions, despite upregulation of adipocyte gene expression. These data suggest that BMP-7 is not a singular lineage determinant, rather it promotes both chondrogenic and osteogenic differentiation of MSCs by co-ordinating with initial lineage-specific signals to accelerate cell fate determination. BMP-7 may be a useful enhancer of in vitro differentiation of BM MSCs for cell-based tissue repair.

show abstract

Section: Discussionsupporting

confidence: 93%

mentioning

confidence: 96%

The role of BMP‐7 in chondrogenic and osteogenic differentiation of human bone marrow multipotent mesenchymal stromal cells in vitro

Shen

Wei

Whittaker

et al. 2009

J of Cellular Biochemistry

142

View full text Add to dashboard Cite

show abstract

“…How aged cartilage responds to the effects of BMP-7 is presently undefined. BMP-7 inhibits MSC proliferation but stimulates ECM synthesis in both synovial and bone marrow-derived MSCs [68,84]. Chondrogenic differentiation of MSCs is enhanced when BMP-7 is in combination with TGF-b3.…”

Section: Bone Morphogenetic Protein-2mentioning

confidence: 98%

The Role of Growth Factors in Cartilage Repair

Fortier

Barker

Strauss

et al. 2011

Clinical Orthopaedics &Amp; Related Research

542

449

View full text Add to dashboard Cite

show abstract

“…IL-6, IL-8, IL-11) and their downstream signaling molecules (receptors, transcription factors, and mitogen-activated kinases), and blocks both a baseline and cytokine-induced expression of MMP-1 and MMP-13 (151). BMP-7 has also been shown to enhance the gene expression of the anabolic molecule tissue inhibitor of metalloproteinase (TIMP) in normal and OA chondrocytes (151), and acts synergistically with the anabolic growth actors IGF-1 (169) and TGF- (172). Data from animal studies reveal that BMP-7 clearly has therapeutic potential for cartilage repair.…”

Section: Bmp-7mentioning

confidence: 94%

Biochemical Mediators Involved in Cartilage Degradation and the Induction of Pain in Osteoarthritis

Ellman¹,

Yan²,

Chen³

et al. 2012

Principles of Osteoarthritis- Its Definition, Character, Derivation and Modality-Related Recognition

View full text Add to dashboard Cite

Osteogenic protein-1 with transforming growth factor-β1: potent inducer of chondrogenesis of synovial mesenchymal stem cells in vitro

Cited by 41 publications

References 29 publications

The role of BMP‐7 in chondrogenic and osteogenic differentiation of human bone marrow multipotent mesenchymal stromal cells in vitro

The role of BMP‐7 in chondrogenic and osteogenic differentiation of human bone marrow multipotent mesenchymal stromal cells in vitro

The Role of Growth Factors in Cartilage Repair

Biochemical Mediators Involved in Cartilage Degradation and the Induction of Pain in Osteoarthritis

Contact Info

Product

Resources

About