Osteogenic potential of embryonic stem cells in tooth sockets

Abstract

Abstract. Embryonic stem cells (ESCs) are established from blastocysts and give rise to various types of cells and tissues. In the present study, we assessed the osteogenic potential of ESCs using in vitro culture conditions and in vivo differentiation in tooth sockets. An ESC-derived embryoid body (EB) was formed and subsequently induced to an osteogenic lineage. The differentiated EB cells exhibited increased expression of various osteogenic markers as determined by real-time PCR analysis. Likewise, the differentiated EB-derived cells had enhanced alkaline phosphatase activity and calcium accumulation, as determined by cytochemical methods. For in vivo transplantation, mixtures of ESCs and hydroxyapatite/ tricalcium phosphate particles or EBs alone were transplanted into female rat tooth sockets. After 12 weeks, we observed formation of osteogenic structure in the tooth sockets without evidence of teratomas. These data suggest that pluripotent ESCs can serve as an alternative source for the reconstruction of craniofacial structures, as well as for further applications.

A laminin‐211‐derived bioactive peptide promotes the osseointegration of a sandblasted, large‐grit, acid‐etched titanium implant

“…ALP is an early‐stage marker of bone formation, and increased ALP activity is associated with osteoblast differentiation (Fromigue, Hamidouche, Chateauvieux, Charbord, & Marie, ; Kang et al, ; Turksen, Bhargava, Moe, & Aubin, ). To determine the effect of the Ln2‐P3 treatment of SLA Ti surfaces on osteoblast differentiation, MG63 cells were incubated with untreated or Ln2‐P3‐treated disc surfaces for 1 day.…”

“…Fromigue, Hamidouche, Chateauvieux, Charbord, & Marie, 2008;Kang et al, 2008;Turksen, Bhargava, Moe, & Aubin, 1992). To determine the effect of the Ln2-P3 treatment of SLA Ti surfaces on osteoblast differentiation, MG63 cells were incubated with untreated or Ln2-P3-treated disc surfaces for 1 day.…”

Stem Cells Nurturing Our Today and Tommorrow

“…Up to now, only isolated studies on animal embryonic stem cells have been conducted . 12,13,14,15 Tissue samples from various -parent‖ tissues can serve as the source for harvesting adult stem cells. Adult stem cells can only proliferate a limited number of times.…”

Dental pulp stem cells: Potential significance in regenerative medicine

“…Smatra se da će embrionalne matične ćelije u budućnosti biti jedan od alternativnih izvora za rekonstrukciju kraniofacijalnih struktura, s obzirom da je dokazan njihov osteogeni potencijal u uslovima kultivisanja, dok se u uslovima in vivo ove ćelije diferenciraju u koštano tkivo u zubnoj alveoli pacova, bez pojave teratoma 10 . Matične ćelije zubne pulpe lokalizovane su u perivaskularnoj niši i slične su BMSC.…”