Osteogenic potential of autogenic demineralized dentin implanted in bony defects in dogs

Catanzaro-Guimarães, S. A.; Catanzaro, Bernadete P.N.; Garcia, Guimarães R.B.; Alle, N

doi:10.1016/s0300-9785(86)80136-3

Cited by 36 publications

(42 citation statements)

References 30 publications

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“…The presence of these growth factors in bone matrix and dentin matrix was reported by Bessho et al 1 (1990), Carvalho et al 2 (2004), Catanzaro-Guimarães et al 4 (1986), and Catanzaro-Guimarães 3 (1993), who also explained their osteoinductive activity.…”

Section: Discussionmentioning

confidence: 58%

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Densitometric analysis of the autogenous demineralized dentin matrix on the dental socket wound healing process in humans

et al. 2006

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ABSTRACT:The aim of this study was to evaluate the effects of the autogenous demineralized dentin matrix (ADDM) on the third molar socket wound healing process in humans, using the guided bone regeneration technique and a polytetrafluoroethylene barrier (PTFE). Twenty-seven dental sockets were divided into three groups: dental socket (Control), dental socket with PTFE barrier (PTFE), and dental socket with ADDM slices associated to PTFE barrier (ADDM + PTFE). The dental sockets were submitted to radiographic bone densitometry analysis and statistical analysis on the 15 th , 30 th , 60 th and 90 th days using analysis of variance (ANOVA) and Tukey's test (p ≤ 0.05). The radiographic analysis of the ADDM + PTFE group showed greater homogeneity of bone radiopacity than the Control group and the PTFE group, during all the observation times. The dentin matrix gradually disappeared from the dental socket during the course of the repair process, suggesting its resorption during the bone remodeling process. It was concluded that the radiographic bone density of the dental sockets treated with ADDM was similar to that of the surrounding normal bone on the 90 th day. The ADDM was biocompatible with the bone tissue of the surgical wounds of human dental sockets. The radiographic analysis revealed that the repair process was discreetly faster in the ADDM + PTFE group than in the Control and PTFE groups, although the difference was not statistically significant. In addition, the radiographic image of the ADDM + PTFE group suggested that its bone architecture was better than that of the Control and PFTE groups. DESCRIPTORS: Bone regeneration; Dentin; Tissue engineering. RESUMO:O objetivo desta pesquisa foi avaliar a reparação óssea em alvéolos dentários após exodontia dos terceiros molares inferiores em humanos, com implantação de matriz dentinária desmineralizada autógena (MDDA) na cavidade e cobertura desta com barreira de politetrafluoretileno (PTFE). Foram selecionados 27 dentes, os quais foram divididos em três grupos: alvéolo dentário (Controle), alvéolo dentário com barreira de PTFE (PTFE) e alvé-olo dentário com fatias de MDDA associada à barreira de PTFE (MDDA + PTFE). O alvéolo dentário foi submetido à análise de densitometria radiográfica e à análise estatística no 15º, 30º, 60º e 90º dias, utilizando-se a análise de variância (ANOVA) e teste de Tukey (p ≤ 0,05). A análise radiográfica do grupo MDDA + PTFE mostrou maior homogeneidade na radiopacidade do trabeculado ósseo do que no grupo controle e grupo PTFE, durante todos os períodos de observação. A matriz dentinária foi desaparecendo do alvéolo dentário durante a evolução do processo de reparo, sugerindo sua reabsorção durante o processo de remodelação óssea. Concluiu-se que a densidade óssea radiográfica do alvéolo dentário tratado com MDDA foi similar à do osso normal circunjacente no 90º dia. A MDDA foi biocompatível com o tecido ósseo, quando implantada nos alvéolos dentários cruentos de humanos. Na análise radiográfica, pode-se verificar que o processo de r...

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Section: Discussionmentioning

confidence: 58%

“…4 Several researches have presented some materials that have osteopromotive potential for osteogenesis. The dentin matrix used as implant biomaterial has osteogenic and chemotactic potential.…”

Section: Introductionmentioning

confidence: 99%

Densitometric analysis of the autogenous demineralized dentin matrix on the dental socket wound healing process in humans

et al. 2006

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“…Some authors reported the presence of these growth factors in bone matrix and dentin matrix, which can explain their osteoinductive activity. [10][11][12] During all observation periods of the control group, an average optical density that increased gradually and progressively (Table 1) was observed. Moreover, it was noticed that the PTFe barrier promoted an osteoconduction property, which influenced bone growth positively.…”

Section: Discussionmentioning

confidence: 92%

“…It is known, however, that many chronic complications of Diabetes mellitus patients are related to changes in bone metabolism, since it may impair the bone repair process. 5,9 In the last years, researchers have added growth factors to occlusive barriers such as insulin growth factor, growth factor related to platelets, and bone morphogenetic proteins, among others, 4,10 to accelerate the bone repair process. Some authors reported the presence of these growth factors in bone matrix and dentin matrix, which can explain their osteoinductive activity.…”

Section: Discussionmentioning

confidence: 99%

Optical density of bone repair after implantation of homogenous demineralized dentin matrix in diabetic rabbits

Gomes¹,

Destro

Banzi³

et al. 2008

Braz. oral res.

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This research evaluated the bone repair process after implantation of homogenous demineralized dentin matrix (HDDM) in surgical defects in the parietal bone of rabbits with alloxan-induced diabetes, using a polytetrafluorethylene (PTFe) barrier for guided bone regeneration. Thirty-six rabbits were used and divided into four groups: control (C, n = 12), diabetic (D, n = 12, left parietal bone), diabetic with PTFe (DPTFe, same 12 rabbits, right parietal bone), and diabetic with PTFe associated to HDDM (D-PTFe+HDDM, n = 12). Bone defects were created in the parietal bone of the rabbits and the experimental treatments were performed, where applicable. The rabbits were sacrificed after 15, 30, 60 and 90 days. The bone defects were examined radiographically and by optical density (ANOVA and Tukey test, p < .05). The radiographic findings showed that the D-PTFe+HDDM group presented greater radiopacity and better trabecular bone arrangement when compared to that of the C, D and D-PTFe groups. The statistical analysis showed significant differences in the optical density of the newly formed bone among the studied groups. It was possible to conclude that HDDM was biocompatible in diabetic rabbits.

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“…Although there are several reports on the composition (organic versus inorganic substance) and mechanical properties of the DDM, there is little evidence at the cellular level about the osteogenic potential of the DDM and their bone-forming capacity in vitro, such as in human osteoblast-like cells (MG-63 cells) [19][20][21] .…”

Section: Introductionmentioning

confidence: 99%

Osteogenic Potential of Demineralized Dentin Matrix as Bone Graft Material

Hyo-Jung

Hong

Kim

et al. 2017

J. Hard Tissue Biology.

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The aim of this study was to evaluate the osteogenic potential of demineralized dentin matrix (DDM) in supporting the osteogenic activity of MG-63 cells and compare it to a mixture of inorganic bovine bone and collagen (Bio-Oss Collagen ® ). Cell morphology, differentiation, adhesion, and growth were investigated via scanning electron microscopy and immunofluorescence analyses of F-actin, osteocalcin, and osteonectin. Cell adhesion and growth on the DDM as a bone graft material were more abundant, with cells adopting a flat shape and uniform distribution, than on the Bio-Oss Collagen ® at all the observation time points (i.e., 6, 12, 24, 48, 72 h and 14 days). Cell attachment and cytoskeleton organization assessed using confocal laser scanning microscopy revealed that the DDM provided better attachment with cytoplasmic propagation than the control. Immunofluorescence assays showed that the fluorescent intensities of osteocalcin and osteonectin as biomarkers of cellular differentiation were higher on the DDM than on Bio-Oss Collagen ® at 6, 12 and 24 h. We showed that the DDM had an enhanced osteogenic potential as a bone substitute for bone augmentation procedures in the dental and medical fields.

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Osteogenic potential of autogenic demineralized dentin implanted in bony defects in dogs

Cited by 36 publications

References 30 publications

Densitometric analysis of the autogenous demineralized dentin matrix on the dental socket wound healing process in humans

Densitometric analysis of the autogenous demineralized dentin matrix on the dental socket wound healing process in humans

Optical density of bone repair after implantation of homogenous demineralized dentin matrix in diabetic rabbits

Osteogenic Potential of Demineralized Dentin Matrix as Bone Graft Material

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