Osteogenic Induction of Human Mesenchymal Stem Cells by Cold Atmospheric Argon Plasma

Abstract

Cold plasmas induce angiogenesis, enhance coagulation and wound healing, and selectively ablate microbes without harming eukaryotes. Work on bone tissue has been minimal; initial studies show enhanced osseointegration, increased gene transcription, and mesenchymal stem cell growth. Mesenchymal stem cell osteoblastic differentiation is required for bone formation and healing. The present study employs a novel device to assess whether cold argon plasma induces osteogenic differentiation of human mesenchymal stem cells. Human ionized argon gas, argon plasma, and argon plasma with osteogenic stimulation. Known osteoblastic differentiation markers (alkaline phosphatase, osteocalcin, RANKL) were assessed on days 1, 10, and 28. Cellular DNA production was measured for normalization. Novel dielectric distance 22 mm, and duration 30 sec. Alkaline phosphatase level was decreased compared to line phosphatase (p < 0.0014) compared to other groups. Osteogenic stimulation did not result in difference from growth. Changes in osteocalcin or receptor activator of nuclear factor kappa-B ligand (RANKL) were not observed. plasma to induce osteoblastic differentiation cannot be made. Lack of-glycerophosphate addition on day 14 prevented osteogenic media from responding as expected. Interestingly, nonpossibly due to argon shielding or shear force production, merits further study.

Non-thermal atmospheric-pressure plasma potentiates mesodermal differentiation of human induced pluripotent stem cells

“…Plasma generation technology is continuously improving ( Kumagai et al., 2016 ; Kobayashi et al., 2016 ) and several generators are now commercially available for wound treatment ( Stratmann et al., 2020 ; Shimizu, 2021 ). Applications for biomedical research have been widely investigated ( Ahmed et al., 2016 , Tominami et al., 2017 , von Woedtke et al., 2020 ). For instance, plasma treatment was reported to enhance the proliferation of various mesoderm-derived human adult stem cells ( Park et al., 2019 ).…”