Non-thermal atmospheric-pressure plasma potentiates mesodermal differentiation of human induced pluripotent stem cells

Kobayashi, Mime; Tomoda, Kiichiro; Morihara, Hirofumi; Asahi, Michio; Shimizu, Tetsuji; Kumagai, Shinya

doi:10.1016/j.heliyon.2022.e12009

Cited by 5 publications

(3 citation statements)

References 38 publications

(53 reference statements)

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“…The specific mechanisms by which NBP affects stem cell differentiation can vary depending on the type of stem cells involved and the experimental conditions. NBP has been proven effectively in promoting the differentiation of various stem cell types, such as mesenchymal stem cells, adipose tissue-derived stem cells, pluripotent stem cells [40][41][42]. In addition, it serves as a potent tool for promoting the proliferation of various adult stem cells derived from mesoderm while preserving their stemness and pluripotency, adding value to stem cell therapy.…”

Section: Discussionmentioning

confidence: 99%

Nonthermal biocompatible plasma in stimulating osteogenic differentiation by targeting p38/ FOXO1 and PI3K/AKT pathways in hBMSCs

Akter,

Kim,

Choi

et al. 2024

J Biol Eng

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Osteoporosis is manifested by decreased bone density and deterioration of bone architecture, increasing the risk of bone fractures Human bone marrow mesenchymal stem cells (hBMSCs)-based tissue engineering serves as a crucial technique for regenerating lost bone and preventing osteoporosis. Non-thermal biocompatible plasma (NBP) is a potential new therapeutic approach employed in several biomedical applications, including regenerative medicine. NBP affects bone remodeling; however, its role in the regulation of osteogenic differentiation in hBMSCs remains largely unexplored. This study aimed to explore the efficiency of NBP in promoting osteogenic differentiation, and the molecular pathways through which these responses occurred in hBMSCs. We found that NBP facilitated osteogenic differentiation through the upregulation of the bone morphogenic protein signal (BMPs) cascade, which in turn induced the expression of p38 and inhibited the forkhead box protein O1 (FOXO1). To further gain insight into the mechanism through which NBP extensively triggers the initiation of osteogenic differentiation in hBMSCs, PI3K/AKT pathway was also analyzed. Overall, these results highlight that NBP enhances osteogenic differentiation in hBMSCs by the stimulation of the p38/FOXO1 through PI3K/AKT signaling pathways. Therefore, the application of NBP in hBMSCs may offer tremendous therapeutic prospects in the treatment of bone regeneration and osteoporosis prevention.

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Section: Discussionmentioning

confidence: 99%

Nonthermal biocompatible plasma in stimulating osteogenic differentiation by targeting p38/ FOXO1 and PI3K/AKT pathways in hBMSCs

Akter,

Kim,

Choi

et al. 2024

J Biol Eng

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“…The results showed a significant increase in the expression of genes related to osteoblast differentiation, indicating the potential of non-thermal plasma treatment as a method for inducing bone regeneration. Another recent study by Mime K et al investigated the potential of non-thermal atmospheric-pressure plasma in promoting mesodermal differentiation of human induced pluripotent stem cells (hiPSCs) [23]. The results showed that plasma-treated hiPSCs exhibited suppression of ectoderm genes such as WNT1 and increased expression of mesoderm genes in embryoid bodies (EBs), suggesting that plasma treatment may be a useful tool to guide the fate of pluripotent stem cells while maintaining genomic integrity.…”

Section: Future Frontiersmentioning

confidence: 99%

Application of non-thermal plasma in medicine: a bibliometric and visualization analysis

Guo,

Yan,

Gong

et al. 2023

Front. Phys.

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Non-thermal plasma has been widely studied in medicine due to its beneficial effect with low thermal or other damages. Numerous medical research studies have been conducted using non-thermal plasma devices; yet, to date, there is no report summarizing this field as a whole. In this study, we aim to perform a bibliometric analysis to assess the state of research, current research priorities, and emerging trends in non-thermal plasma medicine over the last two decades. Publications related to non-thermal plasma medicine (2002–2022) were searched in Web of Science core collection. Bibliometric analysis and visualization was then performed using R-Bibliometrix and CiteSpace. A total of 725 publications related to non-thermal plasma were identified. The annual number of publications has increased continuously over the past two decades. n the field of non-thermal plasma medicine, Germany, China and the United States dominated. Of all institutions, Leibniz Institute for Plasma Science and Technology published the most papers. The journal with highest citation was JOURNAL OF PHYSICS D-APPLIED PHYSICS. Bekeschus Sander published the highest H-value of articles. “Inactivation”, “Apoptosis”, “Chronic Wound” is the primary focus area of non-thermal plasma medicine, “Differentiation”, “Water”, “Gene Expression” and “Cell Death” were the main keywords of the new research hotspots. In this study, bibliometric methods were used to analyze current research priorities and trends in non-thermal plasma medicine and to identify the countries, institutions, authors, and journals with the greatest influence in the field to enhance collaboration and learning.

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“…14) At the laboratory level, plasma has been used for selected cell death, 15,16) as well as the proliferation 17,18) and differentiation of cells. 19,20) Meanwhile, applications in agriculture such as promoting plant growth, [21][22][23] improvement in mushroom cultivation, 24) and improvement in the quality of rice crops 25) have also been reported.…”

Section: Introductionmentioning

confidence: 99%

Microperfusion cell culture system for promoted cell growth using non-thermal atmospheric pressure plasma exposure

Okino

Yamada

Muraki

et al. 2023

Jpn. J. Appl. Phys.

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Promoting cell growth is demanded in various applications such as drug screening and regenerative medicine. Recently, non-thermal atmospheric pressure plasma (NTAPP) which can generate reactive oxygen and nitrogen species under atmospheric conditions has been used to promote cell growth. In this study, micro perfusion systems using stimulation of NTAPP exposure was developed towards promoting cell growth. NTAPP was generated through dielectric barrier discharge method. When the NTAPP exposure was applied to pure water, concentrations of nitrate, nitrite, and hydrogen peroxide reached 250, 20, and 50 mg/L, respectively, within 4 min plasma exposure. In the cell culture experiment, murine myoblast cells C2C12 were injected into the micro perfusion system. C2C12 cells were stimulated with NTAPP exposure and cultured for 3 days. Then, liquid medium was changed to initiate differentiation. Formation of tubular structures were formed after 3-day incubation.

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Non-thermal atmospheric-pressure plasma potentiates mesodermal differentiation of human induced pluripotent stem cells

Cited by 5 publications

References 38 publications

Nonthermal biocompatible plasma in stimulating osteogenic differentiation by targeting p38/ FOXO1 and PI3K/AKT pathways in hBMSCs

Nonthermal biocompatible plasma in stimulating osteogenic differentiation by targeting p38/ FOXO1 and PI3K/AKT pathways in hBMSCs

Application of non-thermal plasma in medicine: a bibliometric and visualization analysis

Microperfusion cell culture system for promoted cell growth using non-thermal atmospheric pressure plasma exposure

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