Osteogenic Gene Expression by Human Periodontal Ligament Cells under Cyclic Tension

Wescott, David C.; Pinkerton, M.N.; Gaffey, Benjamin J.; Beggs, Kyle T.; Milne, Trudy; Meikle, Murray C.

doi:10.1177/154405910708601214

Cited by 122 publications

(136 citation statements)

References 37 publications

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“…Based on the belief that this protein may be a mineralization inhibitor and the fact that the PDL is "sandwiched" between two mineralized tissues, cementum and alveolar bone, it is tempting to think that this protein may be important in preventing PDL from being mineralized. It might provide valuable information about the role of FAM20C as we examine whether the inactivating mutations in the FAM20C gene, as in the case of lethal osteosclerotic bone dysplasia, may result in tooth ankylosis in these patients (Wescott et al 2007) or in animal models.…”

Section: Discussionmentioning

confidence: 99%

Expression of FAM20C in the Osteogenesis and Odontogenesis of Mouse

Wang

Hao

Xie

et al. 2010

J Histochem Cytochem.

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S U M M A R Y Mutations in FAM20C were recently identified as the cause of lethal osteosclerotic bone dysplasia, which highlighted the important role of this molecule in biomineralization. No systematic studies have been performed to evaluate the expression pattern of this relatively new molecule in the developmental processes of bone and tooth. In the present study, we analyzed in detail the expression profile of FAM20C during osteogenesis and odontogenesis using ISH and IHC approaches. The specimens analyzed were mouse tissues spanning embryonic day 13.5 (E13.5) to postnatal 8 weeks. The earliest presence of FAM20C was observed at E14.5. During osteogenesis, FAM20C mRNA was detected in the chondrocytes and osteoblasts of the long bone, whereas its protein was observed in the extracellular matrix (ECM) of bone and in the cytoplasm of the chondrocytes, osteoblasts, and osteocytes. During odontogenesis, FAM20C mRNA was detected in the ameloblasts, odontoblasts, cementoblasts, and periodontal ligament fibroblasts, whereas its protein was observed in the matrices of dentin, enamel, and alveolar bone and in the cytoplasm of the aforementioned cells. The temporospatial expression profile revealed in this study indicates that FAM20C is an ECM protein that may play an important role in controlling the mineralization of bone and tooth. (J Histochem Cytochem 58:957-967, 2010)

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Section: Discussionmentioning

confidence: 99%

Expression of FAM20C in the Osteogenesis and Odontogenesis of Mouse

Wang

Hao

Xie

et al. 2010

J Histochem Cytochem.

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“…In contrast, tension applied to the tooth causes newly alveolar bone to form in the tension side (4). Indeed, 12% uni-axial cyclic tension increases the expression of ALP (7). However, other studies show that cyclic tension decreases ALP activity (8).…”

Section: Introductionmentioning

confidence: 96%

Static tensional forces increase osteogenic gene expression in three-dimensional periodontal ligament cell culture

Ku¹,

Chang²,

Chae³

et al. 2009

BMB Reports

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Orthodontic tooth movement results from the combinational process of both bone resorption and formation in the compressive and tension sides, respectively. However, the genes responsible for new bone formation in tension sides have not been determined. In this study, we used DNA microarray and real-time RT-PCR to identify genes in human periodontal ligament (PDL) cells that undergo significant changes in expression in response to static tensional forces (2 or 12 hours). The genes found were alkaline phospatase (ALP), matrix metalloproteinases (MMPs), vascular endothelial growth factor (VEGF), and several collagen genes. Furthermore, an ELISA evaluating the expression of VEGF, type IV collagen and MMP-2 found levels significantly increased after 24 and 72 hours (P ＜ 0.05). ALP activity was also increased after 24 hours (P ＜ 0.05). Collectively, we found the genes up-regulated in our study by the static tensional force are related to osteogenic processes such as matrix synthesis and angiogenesis. [BMB reports 2009; 42(7): 427-432]

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“…These results suggested an involvement of an ERK1/2-dependent pathway in the ephrin-B2-dependent regulation of Runx2 in osteoblasts. Increased ALP transcription was accompanied by an increase in Ephrin-B2-Fc Induces Runx2 and ALPL Transcription in PDLF-Subpopulations of PDLF have been reported to be able to express osteoblast-specific genes (14) and might contribute to osteogenesis at tension sites in orthodontic tooth movement. Here, we showed that mechanical strain in PDLF induces the expression of ephrin-B2.…”

Section: Ephrin-b2-fc Activates Runx2 and Alp Transcription In Osteobmentioning

confidence: 99%

“…Fibroblasts within the PDL are the first cellular recipients of mechanical strain. Under cyclic strain, PDL fibroblasts in vitro increased their osteogenic gene expression (14). An in vivo model of tooth movement indicated enhanced expression of Runx2 and the phosphorylation of extracellular signal-regulated kinases 1/2 (pERK1/2) under tension (15).…”

mentioning

confidence: 99%

Strain-dependent Up-regulation of Ephrin-B2 Protein in Periodontal Ligament Fibroblasts Contributes to Osteogenesis during Tooth Movement

Diercke

Kohl

Lux

et al. 2011

Journal of Biological Chemistry

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Background: Regulation of bone remodeling during orthodontic tooth movement. Results: Tensile stress induces ephrin-B2 expression in PDL fibroblasts, and ephrin-B2-EphB4 interactions induce osteoblastogenesis in osteoblasts of the alveolar bone. Conclusion: Ephrin-B2-EphB4 signaling between PDLF and osteoblasts of the alveolar bone might contribute to bone remodeling during orthodontic tooth movement. Significance: Understanding the regulation of bone remodeling during orthodontic tooth movement is a prerequisite for pharmacological interventions.

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Osteogenic Gene Expression by Human Periodontal Ligament Cells under Cyclic Tension

Cited by 122 publications

References 37 publications

Expression of FAM20C in the Osteogenesis and Odontogenesis of Mouse

Expression of FAM20C in the Osteogenesis and Odontogenesis of Mouse

Static tensional forces increase osteogenic gene expression in three-dimensional periodontal ligament cell culture

Strain-dependent Up-regulation of Ephrin-B2 Protein in Periodontal Ligament Fibroblasts Contributes to Osteogenesis during Tooth Movement

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