Osteogenic extracellular matrix sheet for bone tissue regeneration

Onishi, Tadanobu; Shimizu, Takamasa; Akahane, Manabu; Omokawa, Shohei; Okuda, Akinori; Kira, Tsutomu; Inagak, Y; Tanaka, Yasuhito

doi:10.22203/ecm.v036a06

Cited by 14 publications

(14 citation statements)

References 35 publications

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“…The implantation of osteogenic ECM sheets (OECMS) that retain the native collagen I and growth factors, together with HA, enhanced bone regeneration in a rat model of femoral non-union at 5 and 8 weeks. The OECMS contained TGF-b and BMP2, leading to increased osteoinduction and osteoconduction (Onishi et al, 2018). When a dECM derived from MG63 cells was deposited on a CS/PCL scaffold, hMSCs exhibited enhanced attachment, proliferation, and osteogenic differentiation, and the scaffold showed anti-inflammatory features in vitro.…”

Section: Cell-derived Decellularized Ecm Scaffoldmentioning

confidence: 99%

The Bone Extracellular Matrix in Bone Formation and Regeneration

et al. 2020

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Bone regeneration repairs bone tissue lost due to trauma, fractures, and tumors, or absent due to congenital disorders. The extracellular matrix (ECM) is an intricate dynamic bio-environment with precisely regulated mechanical and biochemical properties. In bone, ECMs are involved in regulating cell adhesion, proliferation, and responses to growth factors, differentiation, and ultimately, the functional characteristics of the mature bone. Bone ECM can induce the production of new bone by osteoblast-lineage cells, such as MSCs, osteoblasts, and osteocytes and the absorption of bone by osteoclasts. With the rapid development of bone regenerative medicine, the osteoinductive, osteoconductive, and osteogenic potential of ECM-based scaffolds has attracted increasing attention. ECM-based scaffolds for bone tissue engineering can be divided into two types, that is, ECM-modified biomaterial scaffold and decellularized ECM scaffold. Tissue engineering strategies that utilize the functional ECM are superior at guiding the formation of specific tissues at the implantation site. In this review, we provide an overview of the function of various types of bone ECMs in bone tissue and their regulation roles in the behaviors of osteoblast-lineage cells and osteoclasts. We also summarize the application of bone ECM in bone repair and regeneration. A better understanding of the role of bone ECM in guiding cellular behavior and tissue function is essential for its future applications in bone repair and regenerative medicine.

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Section: Cell-derived Decellularized Ecm Scaffoldmentioning

confidence: 99%

The Bone Extracellular Matrix in Bone Formation and Regeneration

et al. 2020

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“…In this study, ICA regulated osteogenic ECM by inducing osteogenic differentiation of BMSCs during ECM formation. In addition, it has been demonstrated that growth factors such as BMP-2 could be retained in decellularized ECM ( Onishi et al, 2018 ).…”

Section: Resultsmentioning

confidence: 99%

Synergistically Promoting Bone Regeneration by Icariin-Incorporated Porous Microcarriers and Decellularized Extracellular Matrix Derived From Bone Marrow Mesenchymal Stem Cells

Zhou

Guo

Shi

et al. 2022

Front. Bioeng. Biotechnol.

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Multifunctionality has becoming essential for bone tissue engineering materials, such as drug release. In this study, icariin (ICA)-incorporated poly(glycolide-co-caprolactone) (PGCL) porous microcarriers were fabricated and then coated with decellularized extracellular matrix (dECM) which was derived from bone marrow mesenchymal stem cells (BMSC). The porous structure was generated due to the soluble gelatin within the microcarriers. The initial released ICA in microcarriers regulated osteogenic ECM production by BMSCs during ECM formation. The dECM could further synergistically enhance the migration and osteogenic differentiation of BMSCs together with ICA as indicated by the transwell migration assay, ALP and ARS staining, as well as gene and protein expression. Furthermore, in vivo results also showed that dECM and ICA exhibited excellent synergistic effects in repairing rat calvarial defects. These findings suggest that the porous microcarriers loaded with ICA and dECM coatings have great potential in the field of bone tissue engineering.

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“…Runx2 represents a vital marker of osteogenesis, and Col1 represents a factor of transcription that underlies the existence of bone cells [ 87 , 88 ]. MSCs are required to be lead to an osteogenic phenotype that can be realized by adding growth factors; another option is their direct cultivation on bone-derived ECM, which presents osteogenic features [ 89 , 90 ].…”

Section: Mesenchymal Stem Cells Influence In Btementioning

confidence: 99%

Bone Regeneration and Oxidative Stress: An Updated Overview

et al. 2022

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Bone tissue engineering is a complex domain that requires further investigation and benefits from data obtained over past decades. The models are increasing in complexity as they reveal new data from co-culturing and microfluidics applications. The in vitro models now focus on the 3D medium co-culturing of osteoblasts, osteoclasts, and osteocytes utilizing collagen for separation; this type of research allows for controlled medium and in-depth data analysis. Oxidative stress takes a toll on the domain, being beneficial as well as destructive. Reactive oxygen species (ROS) are molecules that influence the differentiation of osteoclasts, but over time their increasing presence can affect patients and aid the appearance of diseases such as osteoporosis. Oxidative stress can be limited by using antioxidants such as vitamin K and N‑acetyl cysteine (NAC). Scaffolds and biocompatible coatings such as hydroxyapatite and bioactive glass are required to isolate the implant, protect the zone from the metallic, ionic exchange, and enhance the bone regeneration by mimicking the composition and structure of the body, thus enhancing cell proliferation. The materials can be further functionalized with growth factors that create a better response and higher chances of success for clinical use. This review highlights the vast majority of newly obtained information regarding bone tissue engineering, such as new co-culturing models, implant coatings, scaffolds, biomolecules, and the techniques utilized to obtain them.

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Osteogenic extracellular matrix sheet for bone tissue regeneration

Cited by 14 publications

References 35 publications

The Bone Extracellular Matrix in Bone Formation and Regeneration

The Bone Extracellular Matrix in Bone Formation and Regeneration

Synergistically Promoting Bone Regeneration by Icariin-Incorporated Porous Microcarriers and Decellularized Extracellular Matrix Derived From Bone Marrow Mesenchymal Stem Cells

Bone Regeneration and Oxidative Stress: An Updated Overview

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