Osteogenic effect of tricalcium phosphate substituted by magnesium associated with Genderm® membrane in rat calvarial defect model

Costa, Neusa M.F.; Yassuda, Debora H.; Sader, Marcia S.; Fernandes, Gustavo Vicentis de Oliveira; Soares, Glória Dulce de Almeida; Granjeiro, José Mauro

doi:10.1016/j.msec.2015.12.003

Cited by 28 publications

(42 citation statements)

References 39 publications

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“…At 45 days, in groups I and II the response was of a discrete granulomatous chronic type, characteristics of the foreign body reaction observed after the implantation of biomaterials (Anderson et al, 2008). These results were also seen by Miguel et al (2013) and Costa et al (2016), although these authors have evaluated other types of biomaterials. The absence of rejection by the organism shows that both biomaterials were similarly biocompatible.…”

Section: Discussionmentioning

confidence: 82%

“…On the other hand, the absence of a framework in GIII limited the aforementioned events and resulted in the formation of connective tissue with reduced thickness in relation to the bone edges, mainly in the central region of the defect, at 45 days. Consequently, in this group, the new bone formation was restricted to the borders, throughout the study Miguel et al, 2006Miguel et al, , 2013Tovar et al, 2014;Costa et al, 2016).…”

Section: Discussionmentioning

confidence: 91%

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Biological behavior of magnesium-substituted hydroxyapatite during bone repair

et al. 2021

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The aim of this study was to analyze the biological behavior and osteogenic potential of magnesium (Mg) substituted hydroxyapatite (HA) microspheres, implanted in a critical bone defect, considering that this ion is of great clinical interest, since it is closely associated with homeostasis and bone mineralization. For the purpose of this study, 30 rats were used to compose three experimental groups: GI -bone defect filled with HA microspheres; GII -bone defect filled with HA microspheres replaced with Mg; GIII -empty bone defect; evaluated at biological points of 15 and 45 days. The histological results, at 15 days, showed, in all the groups, a discrete chronic inflammatory infiltrate; biomaterials intact and surrounded by connective tissue; and bone neoformation restricted to the borders. At 45 days, in the GI and GII groups, an inflammatory response of discrete granulomatous chronic type was observed, and in the GIII there was a scarce presence of mononuclear inflammatory cells; in GI and GII, the microspheres were seen to be either intact or fragmented, surrounded by fibrous connective tissue rich in blood vessels; and discrete bone neoformation near the edges and surrounding some microspheres. In GIII, the mineralization was limited to the borders and the remaining area was filled by fibrous connective tissue. It was concluded that the biomaterials were biocompatible and osteoconductive, and the percentage of Mg used as replacement ion in the HA did not favor a greater bone neoformation in relation to the HA without the metal. Comportamento biológico de hidroxiapatita substituída com magnésio durante o reparo ósseoResumo O objetivo deste estudo foi analisar o comportamento biológico de microesferas de hidroxiapatita (HA) substituída com magnésio (Mg) durante o reparo de defeito ósseo crítico, tendo em vista que este íon é de grande interesse clínico, pois está intimamente associado à homeostasia e à mineralização óssea. Para tanto, utilizou-se 30 ratos para compor três grupos experimentais: GI -defeito ósseo preenchido com microesferas de HA; GII -defeito ósseo preenchido com microesferas de HA substituída com Mg; GIII (controle) -defeito ósseo vazio; avaliados nos pontos biológicos de 15 e 45 dias. Os resultados histológicos evidenciaram, aos 15 dias, discreto infiltrado inflamatório crônico e neoformação óssea restrita às bordas, em todos os grupos. Nos grupos GI e GII, os biomateriais mantiveram-se íntegros e circundados por tecido conjuntivo frouxo. Aos 45 dias, notou-se resposta inflamatória do tipo crônica granulomatosa discreta nos grupos GI e GII, e no GIII presença escassa de células inflamatórias mononucleares. As microesferas implantadas no GI e GII mantiveram-se, em sua maioria, íntegras e envolvidas por tecido conjuntivo fibroso. Notou-se discreta neoformação óssea próxima às bordas e circunjacente a algumas microesferas. No GIII, a mineralização limitou-se às bordas e a área remanescente foi preenchida por tecido conjuntivo fibroso. Conclui-se que os biomateriais foram biocompatíveis, bioativos...

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Section: Discussionmentioning

confidence: 82%

Section: Discussionmentioning

confidence: 91%

Biological behavior of magnesium-substituted hydroxyapatite during bone repair

et al. 2021

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“…Critical-size bone defects are considered to have the smallest diameter and do not regenerate spontaneously throughout the life of the animal [21]. erefore, only biomaterials that are truly capable of assisting in bone healing will be significantly superior to the clot-filled control group [14]. e 8 mm diameter bone defect created in the present study is considered critical according to several studies [13,14,21,22] and the results presented here.…”

Section: Discussionmentioning

confidence: 87%

“…However, delayed reabsorption may be detrimental to wound healing due to the formation of degradation products of a nonfunctional membrane [10]. e type I collagen membrane of bovine cortical bone origin is well tolerated by tissues [12][13][14]. Complete reabsorption by mono-and multinucleated cells is evident after 30 to 60 days [15].…”

Section: Introductionmentioning

confidence: 99%

“…Several factors might interfere with bone biodynamics and reestablishment of the bone framework for GBR. e objective of this study was to evaluate the osteopromotor potential of three collagen membranes, two membranes of the bovine cortical bone with different thicknesses (Gen-Derm ® and GenDerm Flex ® ) and a porcine collagen membrane (Bio-Gide ® ), in the process of GBR following installation in critical bone defects in the calvaria of rats [13][14][15][16] at 30 and 60 days using histomorphometric and immunohistochemical analyses.…”

Section: Introductionmentioning

confidence: 99%

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Osteopromotion Capacity of Bovine Cortical Membranes in Critical Defects of Rat Calvaria: Histological and Immunohistochemical Analysis

Danieletto-Zanna

Bizelli

Ramires

et al. 2020

International Journal of Biomaterials

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Membranes that aid the guided bone regeneration (GBR) process have been the subject of studies of compatible biomaterials that contribute to this repair process. The present study compared different membranes used in critical-size defects of rat calvaria by assessing GBR as well as histological, histomorphometric, and immunohistochemical reactions. Forty-eight male albino Wistar rats were randomly allocated into four groups (n = 12 each), namely, C: membrane-free control group (only blood clot, negative control group); BG: porcine collagen membrane group (Bio-Gide®, positive control group); GD: bovine cortical membrane group (first experimental group); and GDF: thicker bovine cortical membrane group (second experimental group). Rats were euthanized at 30 and 60 days postoperatively. Quantitative data from the histometric analysis were submitted to two-way ANOVA and Tukey’s posttest when p<0.05. Histomorphometric results of the thicker bovine cortical membrane at 30 and 60 days were promising, showing improved new bone formation values (p<0.05), and the CD group presented similar results in both analysis periods, being surpassed only by the GDF group (p<0.05). The immunohistochemical results were associated with the histomorphometric data. A less-thick membrane also assisted in GBR. All membranes promoted GBR, especially the positive control and experimental groups.

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Dual‐Controlled Release of Icariin/Mg²⁺ from Biodegradable Microspheres and Their Synergistic Upregulation Effect on Bone Regeneration

Yuan

Wan

Wei

et al. 2020

Adv Healthcare Materials

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Current scaffolds applied for bone tissue engineering are still lacking sufficient osteogenic capacity to induce efficient bone regeneration. Biodegradable microsphere‐type scaffolds are designed to achieve the dual‐controlled release of a Chinese medicine (i.e., icariin, ICA) and a bioactive ion (i.e., Mg2+), in order to achieve their synergistic effect on inducing osteogenesis. The hydrophobic icariin is preloaded onto MgO/MgCO3 (1:1 in weight ratio) particles at different amounts and then the particles are encapsulated into biodegradable poly(lactide‐co‐glycolide) (PLGA) microspheres (PMI) at a fixed fraction (20 wt%). Continuous releases of Mg2+ ion and icariin from the microspheres are detected, showing dependence on icariin amounts. At an optimized moderate loading amount, the resulting PMI‐M microspheres display the strongest activation effect on cell biological behaviors among all the designs. By implanting the PMI‐M microspheres into rat calvarial defects for 16 weeks, it is found that they can effectively enhance new bone formation, presenting significantly higher capacity in inducing osteogenesis than PMg (containing MgO/MgCO3 but without icariin) and blank PLGA microspheres. Clearly, the released Mg2+ ions are beneficial to osteogenesis, and the coincorporation of icariin exerts supplemental effects in inducing new bone formation, which suggest a promising strategy to regenerate severe bone injuries by designing a dual‐release system.

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Osteogenic effect of tricalcium phosphate substituted by magnesium associated with Genderm® membrane in rat calvarial defect model

Cited by 28 publications

References 39 publications

Biological behavior of magnesium-substituted hydroxyapatite during bone repair

Biological behavior of magnesium-substituted hydroxyapatite during bone repair

Osteopromotion Capacity of Bovine Cortical Membranes in Critical Defects of Rat Calvaria: Histological and Immunohistochemical Analysis

Dual‐Controlled Release of Icariin/Mg²⁺ from Biodegradable Microspheres and Their Synergistic Upregulation Effect on Bone Regeneration

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Osteogenic effect of tricalcium phosphate substituted by magnesium associated with Genderm® membrane in rat calvarial defect model

Cited by 28 publications

References 39 publications

Biological behavior of magnesium-substituted hydroxyapatite during bone repair

Biological behavior of magnesium-substituted hydroxyapatite during bone repair

Osteopromotion Capacity of Bovine Cortical Membranes in Critical Defects of Rat Calvaria: Histological and Immunohistochemical Analysis

Dual‐Controlled Release of Icariin/Mg2+ from Biodegradable Microspheres and Their Synergistic Upregulation Effect on Bone Regeneration

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Dual‐Controlled Release of Icariin/Mg²⁺ from Biodegradable Microspheres and Their Synergistic Upregulation Effect on Bone Regeneration