Osteogenic differentiation of stem cells from human exfoliated deciduous teeth on poly(ε-caprolactone) nanofibers containing strontium phosphate

Su, Wen-Ta; Wu, Pai-Shuen; Huang, Te‐Yang

doi:10.1016/j.msec.2014.10.076

Cited by 42 publications

(32 citation statements)

References 39 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…As shown in Figure 2, the PLA-CS nanofiber was found to be suitable for cellular attachment, growth, and viability of HepG2 cells, with only a slight difference compared to growth on 24-well plates. This result is consistent with previous studies of the cellular proliferation on nanofibers (Feng et al, 2010;Su et al, 2015). Following the cultivation period, cellular proliferation increased steadily, and HepG2 cells started to aggregate and formed some cellular clusters such as sphere-like structures.…”

Section: Cellular Proliferation and Aggregation Of Hepg2 Cellssupporting

confidence: 93%

“…Hepatocytes are anchorage cells; therefore, cells and substrate interactions can affect cellular aggregation (Su et al, 2015). The strength of cellular adhesion is a critical factor regulating the development of cellular clusters.…”

Section: Introductionmentioning

confidence: 99%

“…The main characteristic of nanofibers is their ultrafine structure and that the fibers form continuous extensions to construct a threedimensional (3D) scaffold that possesses large porosity, uniform pore distribution, variable pore size, and a high surface-to-volume ratio, showing good potential to effectively mimic a natural environment for cellular growth. Electrospinning is used to transform the biopolymer solution into successive and thin fibers under a high electric field, facilitating the formation of a fibrous scaffold that can act as a substitute for natural extracellular matrices (ECM) to promote cellular growth (Su et al, 2015).…”

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

Nanofibers promote HepG2 aggregate formation and cellular function

Liu

Huang

2016

Genet. Mol. Res.

View full text Add to dashboard Cite

ABSTRACT. Formation of hepatocyte spheroids is a necessary strategy for increasing liver-specific function in vitro. In this study, HepG2 cells showed good viability when grown on a polylactic acid-chitosan (PLA-CS) nanofiber and aggregated to form multicellular spheroids on the PLA-CS nanofibers with a diameter of approximately 100-200 mm in 5 days of culture, whereas no such aggregation was observed in cells cultured on 24-well plates. Hepatocyte spheroids formed on the PLA-CS nanofibers displayed excellent hepatic-related protein expression, such as albumin and urea, compared to HepG2 cells cultured on the 24-well plates. These results indicated that formation of the hepatocyte spheroids in nanofibers can increase and maintain hepatocyte functions for a longer time, supporting a new strategy for bioartificial liver development.

show abstract

Section: Cellular Proliferation and Aggregation Of Hepg2 Cellssupporting

confidence: 93%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Nanofibers promote HepG2 aggregate formation and cellular function

Liu

Huang

2016

Genet. Mol. Res.

View full text Add to dashboard Cite

show abstract

“…También se aprecian las bandas del grupo C-H a 2850 cm −1 , grupo Piranósico a 1069 cm −1 y grupo C-O-C a 1021 cm −1 [32,33]. En la figura 2 (b), se observa el espectro del clorhidrato de tiamina respecto a la membrana pre y post-liberación (ver tabla 3).…”

Section: Análisis De Grupos Funcionales En Materiales Y Membranas Porunclassified

Preparación y caracterización de membranas poliméricas electrohiladas de policaprolactona y quitosano para la liberación controlada de clorhidrato de tiamina / Preparation and Characterization of Electrospun Polymeric Membranes of Polycaprolactone and Chitosan for Controlled Release of Thiamine Chlorhydrate

Cepeda

2016

Cienc. En Desarro.

View full text Add to dashboard Cite

ResumenActualmente existen importantes investigaciones sobre la preparación de membranas porosas bioabsorbibles que permiten la liberación controlada de fármacos y vitaminas. En este estudio se propuso preparar membranas porosas a partir de policaprolactona (PCL) y quitosano (CS) bajo la técnica de electrohilado aplicando diferentes parámetros con el fin de evaluar sus características internas y propiedades para una potencial aplicación como liberador del clorhidrato de tiamina (Vitamina B 1 ). Además se desarrolló el estudio de la cinética de liberación In vitro del clorhidrato de tiamina (TC) con las membranas preparadas. Se obtuvo una membrana polimérica a partir de una disolución de

show abstract

“…Nanofibers that are composed of ultrafine and continuous fibers recently attracted attention in regenerative medicine because of their high ratio of the surface to volume, higher porosity, and a capacity to mimic the natural ECM structure for tissue engineering. Electrospinning uses a high electric field to draw the polymer solution into continuous fibers and can fabricate different fibrous scaffolds that imitate the microstructure of the natural ECM [2].…”

Section: Introductionmentioning

confidence: 99%

Nanofiber containing carbon nanotubes enhanced PC12 cell proliferation and neuritogenesis by electrical stimulation

Shih

2015

BME

Self Cite

View full text Add to dashboard Cite

Abstract. The nervous system is an important regulator of the human body because it adapts our responses to the external environment and provides people the ability of thought, memory, and emotion. PC12 is a cell line that is commonly used to study the behavior of neural differentiation. PC12 cells further differentiate into nerve cells when stimulated by nerve growth factor (NGF), which have neurite, dendrite, and axon, and form synapses with neighboring cells to build neural networks. Micropatterns and electric stimulation can significantly influence cellular attachment, proliferation, orientation, extracellular matrix (ECM) expression, neural differentiation, and cellular motion. We fabricated polycaprolactone (PCL) nanofiber with or without carbon nanotubes (CNTs) by electrospinning and promoted the neural differentiation of PC12 cells by electric stimulation. We used scanning electron microscope (SEM) and fluorescence microscope to observe the NGF-induced growth of PC12 cells on PCL nanofiber. Axon formation and cellular activity expression, that confirm that PC12 cells can grow well on PCL nanofiber, and the gene expressions of MAP1b and GAP43 significantly increased after electric stimulation. Based on the results, the structure of nanofibers containing CNTs can effectively induce neural differentiation of PC12 cells in an electric field. This experimental model can be used for future clinical applications.

show abstract

Osteogenic differentiation of stem cells from human exfoliated deciduous teeth on poly(ε-caprolactone) nanofibers containing strontium phosphate

Cited by 42 publications

References 39 publications

Nanofibers promote HepG2 aggregate formation and cellular function

Nanofibers promote HepG2 aggregate formation and cellular function

Nanofiber containing carbon nanotubes enhanced PC12 cell proliferation and neuritogenesis by electrical stimulation

Contact Info

Product

Resources

About