Osteogenic differentiation of human mesenchymal stem cells in freeze-gelled chitosan/nano β-tricalcium phosphate porous scaffolds crosslinked with genipin

Siddiqui, Nadeem; Pramanik, Krishna; Jabbari, Esmaiel

doi:10.1016/j.msec.2015.05.005

Cited by 55 publications

(36 citation statements)

References 37 publications

(52 reference statements)

Supporting

Mentioning

Contrasting

Order By: Relevance

“…These characteristics enable it to provide temporary support in the mineralisation stage, accelerate the regeneration process, and improve the mechanical capability of the target position [81]. Our preliminary data are consistent with the results of Siddiqui et al [82], and we found the mean mass loss of scaffold in the first month was 15.70 ± 1.24%, indicating that the scaffold has good degradation ability. When the degradation rate of the scaffold matches the osteogenesis rate of the new bone, the scaffold can provide adequate temporary support.…”

Section: Discussionsupporting

confidence: 90%

Indirect 3D printing technology for the fabrication of customised β-TCP/chitosan scaffold with the shape of rabbit radial head—an in vitro study

et al. 2019

View full text Add to dashboard Cite

Background With the development of indirect three-dimensional (3D) printing technology, it is possible to customise individual scaffolds to be used in bone transplantation and regeneration. In addition, materials previously limited to the 3D printing (3DP) process due to their own characteristics can also be used well in indirect 3DP. In this study, customised β-TCP/chitosan scaffolds with the shape of rabbit radial head were produced by indirect 3D printing technology. Methods Swelling ability, porosity, mechanical characterisation, and degradation rate analysis were performed, and in vitro studies were also implemented to evaluate the proliferation and osteogenic differentiation of bone marrow mesenchymal stem cells (MSCs) on the scaffolds. CCK8 cell proliferation assay kit and alkaline phosphatase (ALP) staining solution were used to study cell proliferation and early ALP content at the scaffold surface. Moreover, the osteogenic differentiation of MSCs on scaffolds was also evaluated through the scanning electron microscopy analysis. Results β-TCP/chitosan scaffold has good performance and degradation rate, and in vitro cell experiments also confirm that the scaffold has adequate cytocompatibility and bioactivity. Conclusion This study provides a promising new strategy for the design of customised scaffolds for the repair of complex damaged tissues.

show abstract

Section: Discussionsupporting

confidence: 90%

Indirect 3D printing technology for the fabrication of customised β-TCP/chitosan scaffold with the shape of rabbit radial head—an in vitro study

et al. 2019

View full text Add to dashboard Cite

show abstract

“…Recent studies also show that β-TCP promotes hBMSC proliferation and differentiation 39 . The injectable PCL/β-TCP composites tested in this study have been shown to be able to release both calcium ions, perhaps because the surface-mediated Ca ion exchange affects cell behavior.…”

Section: Discussionmentioning

confidence: 56%

Fabrication and characterization of polycaprolactone and tricalcium phosphate composites for tissue engineering applications

Huang

Hsu

Huang

et al. 2017

Journal of Dental Sciences

View full text Add to dashboard Cite

Background/purpose β-Tricalcium phosphate (β-TCP) is an osteoconductive material which has been used for clinical purposes for several years, as is polycaprolactone (PCL), which has already been approved for a number of medical and drug delivery devices. In this study we have incorporated various concentrations of β-TCP into PCL with the aim of developing an injectable, mechanically strong, and biodegradable material which can be used for medical purposes without organic solvents. Materials and methods This study assesses the physical and chemical properties of this material, evaluates the in vitro bioactivity of the PCL/β-TCP composites, and analyzes cell proliferation and osteogenic differentiation when using human bone marrow mesenchymal stem cells (hBMSCs). Results The results show that weight losses of approximately 5.3%, 12.1%, 18.6%, and 25.2%, were observed for the TCP0, TCP10, TCP30, and TCP50 composites after immersion in simulated body fluid for 12 weeks, respectively, indicating significant differences (P < 0.05). In addition, PCL/β-TCP composites tend to have lower contact angles (47 ± 1.5° and 58 ± 1.7° for TCP50 and TCP30, respectively) than pure PCL (85 ± 1.3°), which are generally more hydrophilic. After 7 days, a significant (22% and 34%, respectively) increase (P < 0.05) in alkaline phosphatase level was measured for TCP30 and TCP50 in comparison with the pure PCL. Conclusion PCL/TCP is biocompatible with hBMSCs. It not only promotes proliferation of hBMSCs but also helps to differentiate reparative hard tissue. We suggest 50% (weight) PCL-containing β-TCP biocomposites as the best choice for hard tissue repair applications.

show abstract

“…Cell adhesion is enhanced via surface modifying CS using integrin binding ligands or proteins with Arginine–Glycine–Aspartic acid (RGD) binding sequence . Fibrin is one such protein with plenty of RGD sequences, which when coated on genipin cross‐linked CS/nano β‐tricalcium phosphate composite scaffold displayed higher cell attachment . RGD functionalized CS derivative, carboxymethyl‐trimethyl CS (CM‐TM‐CS), showed a drastic improvement in cell adhesion due to the presence of RGD‐peptides, which facilitated the binding to the cells to attain a well‐spread morphology ( Figure ) .…”

Section: Surface Modification Of Cs Biocomposites In Btementioning

confidence: 99%

Chitosan in Surface Modification for Bone Tissue Engineering Applications

Abinaya

Prasith

Ashwin

et al. 2019

Biotechnology Journal

View full text Add to dashboard Cite

Traditional methods of bone defect repair include autografts, allografts, surgical reconstruction, and metal implants that have several disadvantages such as donor site morbidity, rejection, risk of disease transmission, and repetitive surgery. Biomaterial‐based bone reconstructions can, therefore, be an efficient alternative due to the inherent properties of the materials. Chitosan (CS), the deacetylated form of chitin, is a biopolymer having a wide array of applicability in regenerative tissue applications owing to its biocompatible, in vitro degradative and bioresorbable nature. Extensive studies are being carried out using CS to augment the properties of the already existing methods and to also improve the applicability of CS‐based biocomposites in bone tissue repair. In this review, the suitability of CS as a surface modifier has been discussed in detail for the already existing implants, surface modifications of CS‐based natural biocomposites for bone tissue regeneration, and the wide range of techniques that can introduce these modifications. CS, being a natural polymer, possesses advantageous properties including surface modifier that makes it a suitable candidate for bone regeneration, and further research to investigate its osteogenic potential in vivo along with the molecular and signaling mechanisms involved in bone regeneration can aid in expanding its applicability in clinical trials.

show abstract

Osteogenic differentiation of human mesenchymal stem cells in freeze-gelled chitosan/nano β-tricalcium phosphate porous scaffolds crosslinked with genipin

Cited by 55 publications

References 37 publications

Indirect 3D printing technology for the fabrication of customised β-TCP/chitosan scaffold with the shape of rabbit radial head—an in vitro study

Indirect 3D printing technology for the fabrication of customised β-TCP/chitosan scaffold with the shape of rabbit radial head—an in vitro study

Fabrication and characterization of polycaprolactone and tricalcium phosphate composites for tissue engineering applications

Chitosan in Surface Modification for Bone Tissue Engineering Applications

Contact Info

Product

Resources

About