Indirect 3D printing technology for the fabrication of customised β-TCP/chitosan scaffold with the shape of rabbit radial head—an in vitro study

Wang, Ji-Qi; Jiang, Bingjie; Guo, Weijun; Zhao, You-Ming

doi:10.1186/s13018-019-1136-7

Cited by 20 publications

(20 citation statements)

References 82 publications

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“…The manipulation of three-dimensional models seems to facilitate the diagnosis and reproducibility using the AO/OTA and Neer Classifications, 1970 [5]. In the case of surgical treatments, preoperative planning can be carried out with 3D-models, allowing the surgeon to train strategies and maneuvers to reposition the deviated bone fragments and choose the implants for each patient before surgery [11,18,19]. The choice involves size and models of plates or nails, screws, and others.…”

Section: Discussionmentioning

confidence: 99%

“…Slings, plates, nails, and prostheses are nowadays the therapeutic arsenal used to correlate the patient’s fracture with the surgeon’s skills to decide on the best treatment. Thus, research on topics involving new classifications or diagnostic methods have been presented [ 6 , 13 , 18 , 20 – 24 , 29 , 30 ] [ 6 , 13 , 18 , 20 – 24 , 29 , 30 ] and studies with 3D-models are promising [ 11 – 13 , 19 , 31 , 32 ].…”

Section: Discussionmentioning

confidence: 99%

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Three-dimensional models increase the interobserver agreement for the treatment of proximal humerus fractures

et al. 2020

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Background: The agreement for the treatment of proximal humerus fractures is low. Interpretation of exams used for diagnosis can be directly associated with this limitation. This study proposes to compare the agreement between experts and residents in orthopedics for treatment indication of proximal humerus fractures, utilizing 3Dmodels, holography (augmented reality), x-rays, and tomography as diagnostic methods. Methods: Twenty orthopedists (ten experts in shoulder and elbow surgery and ten experts in traumatology) and thirty resident physicians in orthopedics evaluated nine fractures of the proximal humerus, randomly distributed as x-rays, tomography, 3D-models and holography, using the Neer and AO / OTA Classifications. After, we evaluated the interobserver agreement between treatment options (conservative, osteosynthesis and arthroplasty) and whether the experience of the evaluators interfered with the results. Results: The interobserver agreement analysis showed the following kappa-values: κ = 0.362 and κ = 0.306 for experts and residents (3D-models); κ = 0.240 and κ = 0.221 (X-ray); κ = 0.233 and κ = 0.123 (Tomography) and κ = 0.321 and κ = 0.160 (Holography), for experts and residents respectively. Moreover, residents and specialists were discordant in the treatment indication using Tomography as a diagnostic method (p = 0.003). The same was not seen for the other diagnostic methods (p > 0.05). Conclusions: Three-dimensional models showed, overall, the highest interobserver agreement (experts versus residents in orthopedics) for the choice of treatment of proximal humerus fractures compared to X-ray, Tomography, and Holography. Agreement in the choice of treatment among experts that used Tomography and Holography as diagnostic methods were two times higher compared to residents.

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Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Three-dimensional models increase the interobserver agreement for the treatment of proximal humerus fractures

et al. 2020

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“…This may due to the osteogenic ability of the CS-b-TCP layer, as it is capable of promoting attachment and osteogenesis of BMSCs. 26 From the results of histological examination (Fig. 6c, g), the defects treated by the bi-layer scaffold were filled with the newly formed bone, whereas the defects treated by the CS scaffold were incompletely filled.…”

Section: Disscussionmentioning

confidence: 93%

“…24 To overcome the disadvantage of the inorganic ceramic material, b-TCP is often combined with other organic biopolymer materials. 25 It has been proved that scaffolds with b-TCP and CS can not only promote the adhesion and proliferation [25][26][27] but also promote the osteogenic differentiation of osteoblasts and bone marrow stem cells (BMSCs). 26,27 Therefore, the bi-layered composite scaffold with CS acted as a cartilage layer and CS-b-TCP as a bone layer was fabricated in this study.…”

Section: Introductionmentioning

confidence: 99%

Bi-layered Composite Scaffold for Repair of the Osteochondral Defects

Cheng

Dai

et al. 2021

Advances in Wound Care

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Objective: Osteochondral defect presents a big challenge for clinical treatment. This study aimed at constructing a bi-layered composite chitosan/ chitosan-b-tricalcium phosphate (CS/CS-b-TCP) scaffold and at repairing the rat osteochondral defect. Approach: The bi-layered CS/CS-b-TCP scaffold was fabricated by lyophilization, and its microstructure was observed by a scanning electron microscope. Chondrocytes and bone marrow stem cells (BMSCs) were seeded into the CS layer and the CS-b-TCP layer, respectively. Viability and proliferation ability of the cells were observed under a confocal microscope. After subcutaneous implantation, the chondrogenic ability of the CS layer and osteogenic ability of the CS-b-TCP layer were evaluated by immunofluorescence. Then, the bilayered scaffolds were implanted into the rat osteochondral defects and the harvested samples were macroscopically and histologically evaluated. Results: The bi-layered CS/CS-b-TCP scaffold exhibited the distinctive microstructures for each layer. The seeded chondrocytes in the CS layer could maintain the chondrogenic lineage, whereas BMSCs in the CS-b-TCP layer could continually differentiate into the osteogenic lineage. Moreover, cells in both layers could maintain well viability and excellent proliferation ability. For the in vivo study, the newly formed tissues in the bi-layered scaffolds group were similar with the native osteochondral tissues, which comprised hyaline-like cartilage and subchondral bone, with better repair effects compared with those of the pure CS group and the blank control group. Innovation: This is the first time that the bi-layered composite CS/CS-b-TCP scaffold has been fabricated and evaluated with respect to osteochondral defect repair. Conclusion: The bi-layered CS/CS-b-TCP scaffolds could facilitate osteochondral defect repair and might be the promising candidates for osteochondral tissue engineering.

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“…Moreover, the β-TCP-PLA mixture membrane has been demonstrated to favor bone healing in the anterior esthetic area of the maxilla in a recent clinical study (Canullo et al, 2019). It has also been revealed that β-TCPchitosan composite scaffolds can significantly enhance the proliferation and osteogenic differentiation of BMSCs in vitro (Wang et al, 2019a). Additionally, the addition of pulverized human bone into β-TCP-chitosan composite scaffolds presented excellent mechanical properties and induced significantly higher ALP activity than β-TCP-chitosan composite scaffolds when seeded with MG63 cells (Kowalczyk et al, 2021).…”

Section: Beta-tricalcium Phosphate In Combination With Other Materialsmentioning

confidence: 97%

Current Application of Beta-Tricalcium Phosphate in Bone Repair and Its Mechanism to Regulate Osteogenesis

Zhou

et al. 2021

Front. Mater.

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Large segmental bone loss and bone resection due to trauma and/or the presence of tumors and cysts often results in a delay in healing or non-union. Currently, the bone autograft is the most frequently used strategy to manage large bone loss. Nevertheless, autograft harvesting has limitations, namely sourcing of autograft material, the requirement of an invasive procedure, and susceptibility to infection. These disadvantages can result in complications and the development of a bone substitute materials offers a potential alternative to overcome these shortcomings. Among the biomaterials under consideration to date, beta-tricalcium phosphate (β-TCP) has emerged as a promising material for bone regeneration applications due to its osteoconductivity and osteoinductivity properties as well as its superior degradation in vivo. However, current evidence suggests the use β-TCP can in fact delay bone healing and mechanisms for this observation are yet to be comprehensively investigated. In this review, we introduce the broad application of β-TCP in tissue engineering and discuss the different approaches that β-TCP scaffolds are customized, including physical modification (e.g., pore size, porosity and roughness) and the incorporation of metal ions, other materials (e.g., bioactive glass) and stem cells (e.g., mesenchymal stem cells). 3D and 4D printed β-TCP-based scaffolds have also been reviewed. We subsequently discuss how β-TCP can regulate osteogenic processes to aid bone repair/healing, namely osteogenic differentiation of mesenchymal stem cells, formation of blood vessels, release of angiogenic growth factors, and blood clot formation. By way of this review, a deeper understanding of the basic mechanisms of β-TCP for bone repair will be achieved which will aid in the optimization of strategies to promote bone repair and regeneration.

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Indirect 3D printing technology for the fabrication of customised β-TCP/chitosan scaffold with the shape of rabbit radial head—an in vitro study

Cited by 20 publications

References 82 publications

Three-dimensional models increase the interobserver agreement for the treatment of proximal humerus fractures

Three-dimensional models increase the interobserver agreement for the treatment of proximal humerus fractures

Bi-layered Composite Scaffold for Repair of the Osteochondral Defects

Current Application of Beta-Tricalcium Phosphate in Bone Repair and Its Mechanism to Regulate Osteogenesis

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