Osteogenic differentiation of BMSCs on MoS2 composite nanofibers with different cell seeding densities

Abstract

Molybdenum disulphide (MoS 2 ) exhibits unique properties that are useful for various biomedical applications. Owing to its distinct characteristics and osteogenic differentiation promotion effect, this material has been studied extensively. However, the effect of cell density on osteogenic differentiation between MoS 2 -based biomaterials and cells is still unknown. In this study, we used MoS 2 /polyacrylonitrile (PAN) composite nanofibres as substrates to evaluate the effect of cellular density on the osteogenic differentiation of bone marrow-derived mesenchymal stem cells (BMSCs). We created different experimental groups with increasing cell seeding densities and investigated cellular behaviours, biocompatibility, proliferation and osteogenic properties. The results show that MoS 2 /PAN composite nanofibres can positively regulate osteogenic differentiation. More importantly, in the presence of standard culture conditions, 1.0 × 10 4 cells/cm 2 is the most efficient and suitable cell seeding density for BMSCs osteogenic differentiation. Our findings suggest that the optimal cell density for osteogenesis is vital for the osteogenic differentiation of BMSCs when these cells are cultured onto MoS 2 -based biomaterials.

Integrated contact lens sensor system based on multifunctional ultrathin MoS2 transistors

“… 1 , 2 , 3 , 4 , 5 Stretchable and flexible devices worn on or implanted in the human body with embedded multifunctional biosensors should enable real-time sensing of physiological signals (e.g., heart rate, body temperature, oxygen saturation) and/or ambient conditions (e.g., the surrounding temperature, humidity, and light and audio levels) without affecting the normal body movements. 6 , 7 , 8 , 9 , 10 , 11 , 12 , 13 By integrating and analyzing a large amount of live data, these devices could dramatically enhance the efficiency of healthcare delivery and carry out delivery of medicines more timely, personalized, and precise. 14 , 15 , 16 , 17 , 18 Among all these devices, contact lenses are one of the most popular wearable devices, designed for vision correction, and aesthetic and therapeutic purposes.…”

“…Here, we chose MoS 2 transistors as the core sensing material because of their large surface-to-volume ratio, layered dangling-bond-free surface, tunable bandgap and charge transfer properties, and good biocompatibility. 13 , 49 , 50 , 51 , 52 , 53 , 54 , 55 , 56 , 57 Unlike traditional top-down fabrication 58 , 59 and bottom-up synthesis methods, 60 , 61 , 62 we applied a gold-mediated exfoliation 63 and assembly strategy ( Figure 1 A) to pattern mono-to-few-layered MoS 2 flakes with large lateral size (~200 × 100 μm). The MoS 2 transistors were mechanically reinforced with a thin layer of photo-patternable PI, while the elastic parts were formed using a serpentine gold (Au) and PI mesh structure.…”

Ca2+-supplying black phosphorus-based scaffolds fabricated with microfluidic technology for osteogenesis

“…It is estimated that the annual number of patients who suffer from traumatic injury in the US is over 1 million, and many of which may give rise to devastating bone defects, with costs over $10 billion [ 2 ]. Furthermore, bone defects with a diameter greater than 3 cm are less likely to heal on their own, leading to a high incidence of complications, pain, and lower quality of life [ 3 , 4 ]. Although therapeutic strategies for bone defects such as autografts and allografts have reached clinical practice, several limitations their therapeutic effect.…”

Facile synthesis of an organic/inorganic hybrid 2D structure tincone film by molecular layer deposition

“…Therefore, the thickness and uniformity control during thin film growth is important for the reliability of the 2D material device performance. [7][8][9][10][11] There are two main methods for producing 2D materials: bottom-up and top-down. The most widely used top-down method is micromechanical cleavage (MC) using Scotch® tape to cleave the monolayer or a few layers from the bulk layer materials.…”