Osteogenic Differentiation of adipose mesenchymal stem cells with BMP-2 embedded microspheres in a rotating bed bioreactor

Boehm, Stefanie; Lupu, Yael; Machluf, Marcelle; Kasper, Cornelia

doi:10.1186/1753-6561-5-s8-p74

Cited by 6 publications

(3 citation statements)

References 2 publications

(3 reference statements)

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“…Severity of underlying disease was evaluated by McCabe score [6]. The severity of illness on admission was assessed by using the Simplified Acute Physiology Score II (SAPS II) [7] and during hospitalization by using the Sequential Organ Failure Assessment (SOFA) score [8].…”

Section: Methodsmentioning

confidence: 99%

Factors influencing the implementation of antibiotic de-escalation and impact of this strategy in critically ill patients

Gonzalez¹,

Cravoisy²,

Barraud³

et al. 2013

Crit Care

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IntroductionA rational use of antibiotics is of paramount importance in order to prevent the emergence of multidrug resistant bacteria that can lead to therapeutic impasse, especially in intensive care units (ICUs). A de-escalation strategy is therefore naturally advocated as part of better antibiotics usage. However, the clinical impact of such a strategy has not been widely studied. We aimed to assess the feasibility and the clinical impact of a de-escalation strategy in a medical ICU and to identify factors associated when de-escalation was possible.MethodsWe performed a retrospective study of patients hospitalized in a medical ICU over a period of six months. Independent factors associated with de-escalation and its clinical impact were assessed.ResultsTwo hundred and twenty-nine patients were included in the study. Antibiotics were de-escalated in 117 patients (51%). The appropriateness of initial antibiotic therapy was the only independent factor associated with the performance of de-escalation (OR = 2.9, 95% CI, 1.5-5.7; P = 0.002). By contrast, inadequacy of initial antibiotic therapy (OR = 0.1, 0.0 to 0.1, P <0.001) and the presence of multidrug resistant bacteria (OR = 0.2, 0.1 to 0.7, P = 0.006) prevented from de-escalation. There were no differences in terms of short (ICU) or long-term (at 1 year) mortality rates or any secondary criteria such as ICU length of stay, duration of antibiotic therapy, mechanical ventilation, incidence of ICU-acquired infection, or multi-drug resistant bacteria emergence.ConclusionsDe-escalation appears feasible in most cases without any obvious negative clinical impact in a medical ICU.

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Section: Methodsmentioning

confidence: 99%

Factors influencing the implementation of antibiotic de-escalation and impact of this strategy in critically ill patients

Gonzalez¹,

Cravoisy²,

Barraud³

et al. 2013

Crit Care

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“…In this study, we report pDA-mediated immobilization of bone morphogenetic protein-2 (BMP-2)-derived peptides onto biodegradable polymer [poly(lactic- co -glycolic acid), PLGA] scaffolds for enhancing the osteogenic potential of human ADSCs (hADSCs) in vitro and in vivo. BMP-2 signaling is essential for bone formation in the early stage of healing after bone fracture − and also enhances the differentiation of ADSCs into the osteogenic lineage. − Herein, osteoinductive peptides derived from BMP-2 sequence were efficiently immobilized onto PLGA scaffolds by a single step of pDA-mediated coating. Osteogenic differentiation of hADSCs was examined on pDA-BMP-2-PLGA substrates in vitro.…”

Section: Introductionmentioning

confidence: 99%

Polydopamine-Assisted Osteoinductive Peptide Immobilization of Polymer Scaffolds for Enhanced Bone Regeneration by Human Adipose-Derived Stem Cells

et al. 2013

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Immobilization of osteoinductive molecules, including growth factors or peptides, on polymer scaffolds is critical for improving stem cell-mediated bone tissue engineering. Such molecules provide osteogenesis-stimulating signals for stem cells. Typical methods used for polymeric scaffold modification (e.g., chemical conjugation or physical adsorption), however, have limitations (e.g., multistep, complicated procedures, material denaturation, batch-to-batch inconsistency, and inadequate conjugation) that diminish the overall efficiency of the process. Therefore, in this study, we report a biologically inspired strategy to prepare functional polymer scaffolds that efficiently regulate the osteogenic differentiation of human adipose-derived stem cells (hADSCs). Polymerization of dopamine (DA), a repeated motif observed in mussel adhesive protein, under alkaline pH conditions, allows for coating of a polydopamine (pDA) layer onto polymer scaffolds. Our study demonstrates that predeposition of a pDA layer facilitates highly efficient, simple immobilization of peptides derived from osteogenic growth factor (bone morphogenetic protein-2; BMP-2) on poly(lactic-co-glycolic acid) (PLGA) scaffolds via catechol chemistry. The BMP-2 peptide-immobilized PLGA scaffolds greatly enhanced in vitro osteogenic differentiation and calcium mineralization of hADSCs using either osteogenic medium or nonosteogenic medium. Furthermore, transplantation of hADSCs using pDA-BMP-2-PLGA scaffolds significantly promoted in vivo bone formation in critical-sized calvarial bone defects. Therefore, pDA-mediated catechol functionalization would be a simple and effective method for developing tissue engineering scaffolds exhibiting enhanced osteoinductivity. To the best of our knowledge, this is the first study demonstrating that pDA-mediated surface modification of polymer scaffolds potentiates the regenerative capacity of human stem cells for healing tissue defect in vivo.

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“…Adipose-derived MSC were seeded on Sponceram ® biomaterial and cultured in a rotating-bed bioreactor which induced osteogenic differentiation [Boehm et al, 2011]. The differentiation was enhanced by BMP-2-releasing PLGA microspheres.…”

Section: Mechanical Stimulation: Osteoblast Differentiation In Bioreamentioning

confidence: 99%

A Comprehensive Review on the Role of Various Materials in the Osteogenic Differentiation of Mesenchymal Stem Cells with a Special Focus on the Association of Heat Shock Proteins and Nanoparticles

Patil

Paul²

2014

Cells Tissues Organs

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Mesenchymal stem cells (MSCs) have important roles in the area of regenerative medicine and clinical applications due to their pluripotent nature. Osteogenic differentiation of MSCs has been studied extensively using various stimulants to develop models of bone repair. There are several factors that enhance the differentiation of MSCs into bone tissues. This review focuses on the effects of various inducers on the osteoblast differentiation of MSCs at different stages of cellular development. We discuss the various growth factors, hormones, vitamins, cytokines, chemical stimulants, and mechanical forces applied in bioreactors that play an essential role in the proliferation, differentiation, and matrix mineralization of stem cells during osteogenesis. Various nanoparticles have also been used recently for the same purpose and the results are promising. Moreover, we review the role of various stresses, including thermal stress, and the subsequent involvement of heat shock proteins as inducers of the proliferation and differentiation of osteoblasts. We also report how various proteasome inhibitors have been shown to induce proliferation and osteogenic differentiation of MSCs in a number of cases. In this communication, the role of peptide-based scaffolds in osteoblast proliferation and differentiation is also reviewed. Based on the reviewed information, this article proposes novel possibilities for the enhancement of proliferation, differentiation, and migration of osteoblasts from MSCs.

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Osteogenic Differentiation of adipose mesenchymal stem cells with BMP-2 embedded microspheres in a rotating bed bioreactor

Cited by 6 publications

References 2 publications

Factors influencing the implementation of antibiotic de-escalation and impact of this strategy in critically ill patients

Factors influencing the implementation of antibiotic de-escalation and impact of this strategy in critically ill patients

Polydopamine-Assisted Osteoinductive Peptide Immobilization of Polymer Scaffolds for Enhanced Bone Regeneration by Human Adipose-Derived Stem Cells

A Comprehensive Review on the Role of Various Materials in the Osteogenic Differentiation of Mesenchymal Stem Cells with a Special Focus on the Association of Heat Shock Proteins and Nanoparticles

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