Osteogenic differentiation of adipose-derived stem cells and calvarial defect repair using baculovirus-mediated co-expression of BMP-2 and miR-148b

Liao, Ya-Hsin; Chang, Yu‐Han; Sung, Li‐Yu; Li, Kuei-Chang; Yeh, Chia-Lin; Yen, Tzu‐Chen; Hwang, Shiaw-Min; Lin, Kun‐Ju; Hu, Yu‐Chen

doi:10.1016/j.biomaterials.2014.02.055

Cited by 119 publications

(102 citation statements)

References 48 publications

Supporting

Mentioning

101

Contrasting

Unclassified

Order By: Relevance

“…BVs are gradually garnering attention as miRNA delivery vectors, [49] with a panel of osteogenic miRNAs screened for therapeutic effects in vitro, and reported beneficial bone healing effects when assessed as a cotreatment with bone morphogenetic protein-2 (BMP2) in a nude mice calvarial defect model. [50] Many disadvantages continue to surround the use of viral vectors however including immunogenicity and biodistribution concerns, high production costs and complexity for high-mass production. A nascent alternative are virus-like particles (VLPs), a recombinant version of viral capsids recently explored to deliver miRNAs for the treatment of systemic lupus erythematosus.…”

Section: Viral Vectorsmentioning

confidence: 99%

“…[107] Many distinct scaffold types have been tuned to serve the purpose of miRNA delivery, including several hydrogels, [58,92,106,[108][109][110][111][112][113][114] electrospun fibers, [63,[115][116][117][118] and more prolifically porous or spongy scaffolds. [46,47,50,54,55,96,112,[119][120][121][122][123][124][125][126][127][128][129][130] Before reviewing the details of their applications, summarized in Table 2 and described further in the next section, we will discuss the key characteristics making these materials amenable to miRNA delivery. First very general qualities must be fulfilled: biocompatibility, bioresorbability and ease of fabrication.…”

Section: Scaffolds For Microrna Deliverymentioning

confidence: 99%

“…An alternative method involving ASCs transduced with BVs for codelivery of BMP2 pDNA and miR148b on a gelatin-coated PLGA scaffold was investigated to determine the effect on osteogenic differentiation. [153] The BV hybrid used, composed of flippase recombinase (FLPo) and Frtflanking cassette, has previously shown to substantially prolong transgene expression. [122] Having assessed the osteogenic effect of a number of miRNAs in vitro, miR-148b was chosen as the frontrunner and demonstrated enhanced in vitro osteogenesis when cotransduced with BMP2.…”

Section: Osteogenesis and Bone Repairmentioning

confidence: 99%

“…This study revealed miR-148b directly targets NOG, which negatively regulates BMP2-induced osteogenesis and bone formation. The authors recapitulated the codelivery of BMP2 and miR-148b [153] utilizing an alternative Cre/loxP-based BV vector for stronger transgene expression and reduced cytotoxicity, [122] in an attempt to improve the calvarial bone healing. These scaffolds demonstrated superior bone regeneration and restoration of the bone density to 89% of the original, indicating the potential of this alternative scaffold system.…”

Section: Osteogenesis and Bone Repairmentioning

confidence: 99%

See 3 more Smart Citations

Scaffold‐Based microRNA Therapies in Regenerative Medicine and Cancer

Curtin

Castaño

O’Brien

2017

Adv Healthcare Materials

View full text Add to dashboard Cite

The field of "regenerative medicine" (RM) was conceptually developed to aid the body's natural capacity to self-repair, a capacity which is impaired with age and in cases of disease or injury. [1] RM is a vast and multifaceted area of medicine, often microRNA-based therapies are an advantageous strategy with applications in both regenerative medicine (RM) and cancer treatments. microRNAs (miRNAs) are an evolutionary conserved class of small RNA molecules that modulate up to one third of the human nonprotein coding genome. Thus, synthetic miRNA activators and inhibitors hold immense potential to finely balance gene expression and reestablish tissue health. Ongoing industrysponsored clinical trials inspire a new miRNA therapeutics era, but progress largely relies on the development of safe and efficient delivery systems. The emerging application of biomaterial scaffolds for this purpose offers spatiotemporal control and circumvents biological and mechanical barriers that impede successful miRNA delivery. The nascent research in scaffold-mediated miRNA therapies translates know-how learnt from studies in antitumoral and genetic disorders as well as work on plasmid (p)DNA/siRNA delivery to expand the miRNA therapies arena. In this progress report, the state of the art methods of regulating miRNAs are reviewed. Relevant miRNA delivery vectors and scaffold systems applied to-date for RM and cancer treatment applications are discussed, as well as the challenges involved in their design. Overall, this progress report demonstrates the opportunity that exists for the application of miRNA-activated scaffolds in the future of RM and cancer treatments.Regenerative Medicine

show abstract

Section: Viral Vectorsmentioning

confidence: 99%

Section: Scaffolds For Microrna Deliverymentioning

confidence: 99%

Section: Osteogenesis and Bone Repairmentioning

confidence: 99%

Section: Osteogenesis and Bone Repairmentioning

confidence: 99%

See 2 more Smart Citations

Scaffold‐Based microRNA Therapies in Regenerative Medicine and Cancer

Curtin

Castaño

O’Brien

2017

Adv Healthcare Materials

View full text Add to dashboard Cite

show abstract

“…ADMSC is a multipotent cell that can differentiate into adipogenic, osteogenic, chondrogenic, myogenic, neurogenic and endothelial cells (18,19). Treatment of calvarial defects with ADMSC transplantation has been demonstrated in the literature (20)(21)(22). Daei-farshbaf et al reported that ADMSC seeded on a bioceramic scaffold promoted calvarial bone healing in hypothyroid rats (23).…”

Section: Introductionmentioning

confidence: 99%

Adipose Derived Stem Cells Conditioned Media in Combination with Bioceramic-Collagen Scaffolds Improved Calvarial Bone Healing in Hypothyroid Rats

Sanchooli

Norouzian

Ardeshirylajimi

et al. 2017

Iran Red Crescent Med J

View full text Add to dashboard Cite

Background: Tissue engineering is an available treatment for large bone defects. The therapeutic effects of mesenchymal stem cell (MSC) are mostly attributed to secretion of many cytokines and growth factors. Many factors of MSC secretions accumulate in a conditioned medium and these factors recruit native cells into a defect site to generate new bone tissues. Objectives: The aim of this study was to evaluate the influence of adipose tissue derived MSCs-conditioned medium (ADMSC-CM) on bone repair of rats with critical -size calvarial defect. Methods: This experimental study was performed at Shahid Beheshti University of Medical Sciences, Tehran, Iran, from 2016 to 2017. Conditioned medium was collected from healthy rat adipose tissue derived MSC (ADMSC) at passage four. Calvarial bone defect was created in hypothyroid rats using a dental bur. Sampling was taken by the linear-mono-gram method to determine sample size (n = 6 per group). The rats were divided randomly into four groups based on graft material as follows: empty defect, scaffold (Bio-Oss / type I collagen gel), scaffold / ADMSCs, scaffold /ADMSC-CM. Evaluations were made at 4 and 8 weeks after surgery using stereological analysis. Results: Histological analysis at 8 weeks indicated that the newly regenerated tissue almost covered the defect in the ADMSC-CM group. Stereological analysis showed that ADMSC-CM increased regenerated bone and numbers of osteocytes and osteoblasts compared with the defect and scaffold groups (P < 0.05). Also, bone regeneration was more effective in animals treated with ADMSC-CM than in those received ADMSC. Conclusions: These results suggest an important role for ADMSC-CM in bone regeneration, through trophic impact of its cytokines and growth factors that induce native cell proliferation and migration into the defect. Thus, ADMSC-CM seems to have good potential for application in bone tissue regeneration, in the cases of hypothyroidism.

show abstract

Functional Biomaterials for Bone Regeneration: A Lesson in Complex Biology

Armiento

Hatt

Rosenberg

et al. 2020

Adv Funct Materials

150

View full text Add to dashboard Cite

Bone is a hard yet dynamic tissue with remarkable healing capacities. Research to date has greatly advanced the understanding of how bone heals and has led to marked success in the treatment of bone injuries. Nevertheless, the effective treatment of nonunions and large bone defects continues to present a challenge for orthopedic surgeons. Biomaterials provide researchers with a powerful instrument to potentially guide effective bone tissue regeneration in challenging healing environments. However, the most appropriate biomaterial for bone tissue engineering continues to be an area of intense debate. Indeed, the mechanical properties of real bone can be reproduced in vitro but the development of a functional bone substitute goes beyond the sole mechanical properties. The faithful reproduction of bone as a functional organ requires the combination of different cell types and temporal regulation of the molecular signaling involved during the different stages of bone formation and regeneration. This is not at all a trivial task. Herein, critical aspects of bone healing/regeneration including mechanical loading, inflammation, vascularization, and innervation are described. The success and the challenges behind the development of biomaterials, and the technologies used to functionalize them, in order to support the underlying cellular mechanisms are also highlighted.

show abstract

Osteogenic differentiation of adipose-derived stem cells and calvarial defect repair using baculovirus-mediated co-expression of BMP-2 and miR-148b

Cited by 119 publications

References 48 publications

Scaffold‐Based microRNA Therapies in Regenerative Medicine and Cancer

Scaffold‐Based microRNA Therapies in Regenerative Medicine and Cancer

Adipose Derived Stem Cells Conditioned Media in Combination with Bioceramic-Collagen Scaffolds Improved Calvarial Bone Healing in Hypothyroid Rats

Functional Biomaterials for Bone Regeneration: A Lesson in Complex Biology

Contact Info

Product

Resources

About