Osteogenic Cells on Bio-Inspired Materials for Bone Tissue
Engineering

Vagaska, Barbora; Bačáková, Lucie; Filová, Elena; Balik, K.

doi:10.33549/physiolres.931776

Cited by 123 publications

(24 citation statements)

References 96 publications

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“…Across the structures produced in this study with either method, pore diameters ranged from ∼0.5 mm to ∼2.1 mm and so are within the range found typically in soils ( 24 , 25 ), building materials ( 26 , 27 ), and biomedical materials such as tissue scaffolding ( 28 ). If desirable, it should be possible to produce structures with smaller (or larger) pore sizes by using smaller beads or higher-resolution 3D printing technology.…”

Section: Discussionsupporting

confidence: 64%

Microbial Metal Resistance within Structured Environments Is Inversely Related to Environmental Pore Size

Harvey

Mitzakoff

Wildman

et al. 2021

Appl Environ Microbiol

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The physical environments in which microorganisms naturally reside rarely have homogeneous structure, and changes in their porous architecture can have a profound effect on microbial activities – effects that are not typically captured in conventional laboratory studies. Here, to investigate the influence of environmental structure on microbial responses to stress, we constructed structured environments with different pore properties (determined by X-ray Computed Tomography). First, using glass beads in different arrangements and inoculated with the soil yeast Saitozyma podzolica , increases in the average equivalent spherical diameters (ESD) of a structure’s porous architecture led to decreased survival of the yeast under a toxic metal challenge. This relationship was reproduced when yeasts were introduced into additively-manufactured lattice structures, comprising regular arrays with ESDs comparable to those of the bead structures. The pore ESD-dependency of metal resistance was not attributable to differences in cell density in micro-environments delimited by different pore sizes, supporting the inference that pore size specifically is the important parameter here in determining microbial survival of stress. These findings highlight the importance of the physical architecture of an organism’s immediate environment for its response to environmental perturbation, while offering new tools for investigating these interactions in the laboratory. IMPORTANCE Interactions between cells and their structured environments are poorly understood but have significant implications for organismal success in both natural and non-natural settings. This work uses a multidisciplinary approach to develop laboratory models with which the influence of a key parameter of environmental structure – pore size – on cell activities can be dissected. Using these new methods in tandem with additive manufacturing, we demonstrate that resistance of yeast soil-isolates to stress (from a common metal pollutant) is inversely related to pore size of their environment. This has important ramifications for understanding how microorganisms respond to stress in different environments. The findings also establish new pathways for resolving the effects of physical environment on microbial activity, enabling important understanding that is not readily attainable with traditional bulk-sampling and analysis approaches.

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Section: Discussionsupporting

confidence: 64%

Microbial Metal Resistance within Structured Environments Is Inversely Related to Environmental Pore Size

Harvey

Mitzakoff

Wildman

et al. 2021

Appl Environ Microbiol

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“…Cells interact with the surface via a layer of proteins, which they themselves secrete, e.g., cell fibronectin [ 55 ]. Their adhesion on the surfaces of these proteins is provided for by cell adhesion receptors, mainly integrins [ 38 , 56 , 57 ].…”

Section: Discussionmentioning

confidence: 99%

Amyloid Aggregates of Smooth-Muscle Titin Impair Cell Adhesion

Bobylev

Фадеев

Bobyleva

et al. 2021

IJMS

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Various amyloid aggregates, in particular, aggregates of amyloid β-proteins, demonstrate in vitro and in vivo cytotoxic effects associated with impairment of cell adhesion. We investigated the effect of amyloid aggregates of smooth-muscle titin on smooth-muscle-cell cultures. The aggregates were shown to impair cell adhesion, which was accompanied by disorganization of the actin cytoskeleton, formation of filopodia, lamellipodia, and stress fibers. Cells died after a 72-h contact with the amyloid aggregates. To understand the causes of impairment, we studied the effect of the microtopology of a titin-amyloid-aggregate-coated surface on fibroblast adhesion by atomic force microscopy. The calculated surface roughness values varied from 2.7 to 4.9 nm, which can be a cause of highly antiadhesive properties of this surface. As all amyloids have the similar structure and properties, it is quite likely that the antiadhesive effect is also intrinsic to amyloid aggregates of other proteins. These results are important for understanding the mechanisms of the negative effect of amyloids on cell adhesion.

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“…Surface wettability can be changed by physico-chemical treatments and/or by modulating material porosity, which is often used to enhance the stability of implants in the target tissue [ 85 , 86 ]. Laser-generated micropores of ~90 µm enhance the roughness and wettability of titanium surfaces used for dental or bone implants [ 87 ], with rougher surfaces promoting spreading of osteoprogenitor cells and improving osseointegration [ 88 , 89 ].…”

Section: What Is Different Between Normal Wound Healing and The Fbr? The Implantmentioning

confidence: 99%

Implant Fibrosis and the Underappreciated Role of Myofibroblasts in the Foreign Body Reaction

Noskovičová

Hinz

Pakshir

2021

Cells

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Body implants and implantable medical devices have dramatically improved and prolonged the life of countless patients. However, our body repair mechanisms have evolved to isolate, reject, or destroy any object that is recognized as foreign to the organism and inevitably mounts a foreign body reaction (FBR). Depending on its severity and chronicity, the FBR can impair implant performance or create severe clinical complications that will require surgical removal and/or replacement of the faulty device. The number of review articles discussing the FBR seems to be proportional to the number of different implant materials and clinical applications and one wonders, what else is there to tell? We will here take the position of a fibrosis researcher (which, coincidentally, we are) to elaborate similarities and differences between the FBR, normal wound healing, and chronic healing conditions that result in the development of peri-implant fibrosis. After giving credit to macrophages in the inflammatory phase of the FBR, we will mainly focus on the activation of fibroblastic cells into matrix-producing and highly contractile myofibroblasts. While fibrosis has been discussed to be a consequence of the disturbed and chronic inflammatory milieu in the FBR, direct activation of myofibroblasts at the implant surface is less commonly considered. Thus, we will provide a perspective how physical properties of the implant surface control myofibroblast actions and accumulation of stiff scar tissue. Because formation of scar tissue at the surface and around implant materials is a major reason for device failure and extraction surgeries, providing implant surfaces with myofibroblast-suppressing features is a first step to enhance implant acceptance and functional lifetime. Alternative therapeutic targets are elements of the myofibroblast mechanotransduction and contractile machinery and we will end with a brief overview on such targets that are considered for the treatment of other organ fibroses.

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Osteogenic Cells on Bio-Inspired Materials for Bone Tissue Engineering

Cited by 123 publications

References 96 publications

Microbial Metal Resistance within Structured Environments Is Inversely Related to Environmental Pore Size

Microbial Metal Resistance within Structured Environments Is Inversely Related to Environmental Pore Size

Amyloid Aggregates of Smooth-Muscle Titin Impair Cell Adhesion

Implant Fibrosis and the Underappreciated Role of Myofibroblasts in the Foreign Body Reaction

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