Osteoconductive properties of methylpyrrolidinone chitosan in an animal model

Müzzarelli, Riccardo A.A.; Zucchini, Cinzia; Ilari, Pierluca; Pugnaloni, Armanda; Belmonte, M. Mattioli; Biagini, G.; Castaldini, C.

doi:10.1016/0142-9612(93)90134-n

Cited by 146 publications

(74 citation statements)

References 17 publications

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“…Although this lack of degradation differs from other reports, it is important to note that there are clear differences between our material and that used in other previously published results, such as the degree of deacetylation or modification of chitosan with methylpyrrolidinone. 21 Processing methods or chemical modifications that reduce the degree of crystallinity can be used to accelerate degradation in tissue fluids. 22 Dissimilarities in the scaffold fabrication process may also lead to varying results.…”

Section: Discussionmentioning

confidence: 99%

Evaluation of the biocompatibility of a chitosan scaffold in mice

VandeVord

Matthew

DeSilva

et al. 2001

J. Biomed. Mater. Res.

666

398

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Chitosan scaffolds appear to be suitable for a variety of tissue engineering applications. This study addressed the biocompatibility of chitosan in a mouse implantation model. Porous chitosan scaffolds were implanted in mice, and animals were sacrificed after 1, 2, 4, 8, or 12 weeks. Macroscopic inspection of the implantation site revealed no pathological inflammatory responses. Histological assessment indicated marked neutrophil accumulation within the implant, which resolved with increasing implantation time. Gram staining and limulus assays revealed no evidence of infection or endotoxin. Collagen was observed within the chitosan pore spaces, indicating that connective tissue matrix was deposited within the implant. Angiogenic activity associated with the external implant surface was also observed. Cellular immune responses were determined by lymphocyte proliferation assays, and antibody responses were measured using ELISA techniques. These assays indicated a very low incidence of chitosan-specific reactions. Although there was a large migration of neutrophils into the implantation area, there were minimal signs of any inflammatory reaction to the material itself. This preliminary study demonstrates that chitosan has a high degree of biocompatibility in this animal model. Overall, the findings suggest that chitosan may be suitable for the development of implantable materials.

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Section: Discussionmentioning

confidence: 99%

Evaluation of the biocompatibility of a chitosan scaffold in mice

VandeVord

Matthew

DeSilva

et al. 2001

J. Biomed. Mater. Res.

666

398

View full text Add to dashboard Cite

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“…paste membranes, sponges, fibers and spatial porous structures, such as a scaffold, is especially utilized in orthopedics. 14,15 When supplementing bone defects, the most significant effect is through the use of spatial graphs obtained from chitosan.…”

Section: Properties Of Chitosanmentioning

confidence: 99%

Chitosan and its composites: Properties for use in bone substitution

2017

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For many years, research has been carried out on finding an ideal bone substitute. Chitosan (CTS) is a naturally occurring polysaccharide, obtained mainly from, inter alia, the shells of crustaceans. It is characterized by its high level of biocompatibility, biodegradability and antimicrobial properties as well as its support in the healing of wounds. Chitosan, due to its ability to form porous structures, can be used in the production of scaffolds used in the treatment of bone defects. There are numerous studies on the use of CTS in combination with other substances which aim to improve its biological and mechanical properties.The combination of chitosan with the calcium phosphate hydroxyapatite (HAp) has been extensively tested. The objective of the current studies is to verify the properties of scaffolds consisting of chitosan and other substances like polybutylene succinate, human bone marrow mesenchymal stem cells (hBMSCs), collagen, alginate, transforming growth factor -β (TGF-β), insulin-like growth factor (IGF), platelet-derived growth factor (PDGF) or bone morphogenetic proteins (BMP). The aim of the current research is to develop a scaffold with sufficiently good mechanical properties. Trials are underway with many of the biological and synthetic components affecting the biological properties of chitosan. This will allow for the creation of a substitute that fully meets the conditions for an ideal artificial bone.

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“…A bioresorbable chitosan sponge loaded with various growth factors was used to induce bone forma-tion. This biodegradable and nontoxic natural biopolymer has several biomedical applications: it enhances bone formation in vitro (21) and in vivo (22,23). In addition to these biomedical applications, chitosan regulates release of bioactive agents, including growth factors (24).…”

Section: Introductionmentioning

confidence: 99%

Effect of a chitosan sponge impregnated with platelet-derived growth factor on bone augmentation beyond the skeletal envelope in rat calvaria

et al. 2014

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Abstract:We evaluated the ability of platelet-derived growth factor (PDGF) to promote bone augmentation beyond the skeletal envelope in rat calvaria. The calvariae of 14 rats were exposed, and two plastic caps-one with 0.03% PDGF and a chitosan sponge and one with a chitosan sponge alone-were placed. Microcomputed tomography and histologic sections were used to determine the amount of bone augmentation within the plastic caps. Bone volume was calculated using measurement software. Bone volume and amount of bone augmentation were significantly greater in the PDGF group than in the control group. In conclusion, a chitosan sponge containing 0.03% PDGF enhanced bone formation beyond the skeletal envelope in rat calvaria. (J Oral Sci 56, 23-28, 2014)

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Osteoconductive properties of methylpyrrolidinone chitosan in an animal model

Cited by 146 publications

References 17 publications

Evaluation of the biocompatibility of a chitosan scaffold in mice

Evaluation of the biocompatibility of a chitosan scaffold in mice

Chitosan and its composites: Properties for use in bone substitution

Effect of a chitosan sponge impregnated with platelet-derived growth factor on bone augmentation beyond the skeletal envelope in rat calvaria

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