Osteoclastogenic differentiation of human precursor cells over micro- and nanostructured hydroxyapatite topography

Costa‐Rodrigues, João; Carmo, S.; Perpétuo, I.P.; Monteiro, Fernando J.; Fernandes, Maria Helena

doi:10.1016/j.bbagen.2016.01.014

Cited by 25 publications

(28 citation statements)

References 41 publications

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“…One possible 10µm 7 explanation is that the high roughness of the substrate prevented the OC from sealing the substrate, thus hampering the degradation process, as described in previous works. 6,25 One aspect worth noticing is the fact that the microstructure of the pristine material was revealed intact beneath the cell, thus confirming that there was no damage associated to FIB-sectioning.…”

Section: Resultsmentioning

confidence: 92%

Focus Ion Beam/Scanning Electron Microscopy Characterization of Osteoclastic Resorption of Calcium Phosphate Substrates

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Español

Montúfar

et al. 2017

Tissue Engineering Part C: Methods

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Section: Resultsmentioning

confidence: 92%

Focus Ion Beam/Scanning Electron Microscopy Characterization of Osteoclastic Resorption of Calcium Phosphate Substrates

Díez-Escudero

Español

Montúfar

et al. 2017

Tissue Engineering Part C: Methods

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“…The HA/GH composite granules (10,20,30,40 and 50 wt% GH) was allowed to stand in 1.0 ml PBS (+) for 1, 2, 4, 8 and 24 h. Next, 100 µl of the supernatant was collected and 100 µl of PBS (+) was added. After 24 h, 5 µg/ml collagenase L (Nitta Gelatin Inc, Osaka, Japan) was added to the PBS (+).…”

Section: Degradation Of Gh Microspheres In the Ha/gh Composite Granulesmentioning

confidence: 99%

“…The mixture was then passed through a PD-10 column (GE Healthcare Life Sciences, UK) to isolate the 125 I and the 125 I-labelled bFGF. The 125 I-labelled bFGF (100 µg) was allowed to impregnate the HA/GH composite granules (10,20,30,40, and 50 wt% GH) for 12 h at 4ºC. The supernatant was collected as in the decomposition experiment described above, and after the GH microspheres had been completely degradated the amount of bFGF in the supernatant was quantified by measuring the amount of 125 I using a gamma counter (n=5).…”

Section: Release Of Bfgf From the Ha/gh Composite Granulesmentioning

confidence: 99%

“…HA and the HA/GH composite granules (10,20,30,40, and 50 wt% GH) were placed in a 6 mm diameter cell culture insert (FALCON ® Cell Culture Insert, Corning Co, NY, USA), and the cell suspension was seeded at a density of 4×10 4 cells/well and cultured. After 24 h, the culture medium was replaced with á-MEM containing 10 mM glycerol phosphate, 50 µM L-ascorbic acid, and 100 nM dexamethasone, and the cells were cultured for bone differentiation.…”

Section: Cell Culturementioning

confidence: 99%

“…Manufacture and evaluation of the HA/GH composite granules HA granules (20 mg) were added to a 1.0 ml Eppendorf tube, and GH microsphere at various weight ratios to the HA granules (10,20,30,40, and 50 wt% GH) were added and agitated for 1 min. This composite in which different amounts of GH microspheres were uniformly introduced into the pores of the HA granules (Table 1).…”

Section: Manufacture Of Gh Microspheresmentioning

confidence: 99%

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Evaluation of a Porous Hydroxyapatite Granule and Gelatin Hydrogel Microsphere Composite in Bone Regeneration

Yokota

Matsuno

Tabata

et al. 2017

J. Hard Tissue Biology.

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The purpose of this study was to investigate the property of hydroxyapatite (HA)/gelatin hydrogel (GH) composite granules as a scaffold and controlled-release carrier of basic fibroblast growth factor (bFGF) for bone regeneration. HA granules and GH microsphere at various weight ratios to the HA granules were mixed. The composite provided the controlled release of bFGF in vitro. The critical-sized bone defects of 6 mm diameter were created in the skull of New Zealand white rabbits and the composite granules with or without incorporated bFGF, were implanted into the defects. Bone regeneration and were evaluated by computed tomography and H-E staining. The composite granules incorporating bFGF promoted significantly higher bone regeneration at the defect site as compared to the bFGF-free composites. Further, the amount of new bone increased in proportion to the amount of GH microspheres incorporated into the composite. We conclude that the bFGF-impregnated HA/GH composite granules is useful for bone regeneration.

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Distinct Morphologies of Bone Apatite Clusters in Endochondral and Intramembranous Ossification

et al. 2022

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acid) [2] have been widely applied for this purpose. Nevertheless, these materials present limitations related to mimicking the biological and/or biomechanical properties of natural bone apatite. For instance, although hydroxyapatite-based bone mimetics present chemical similarities with natural bone, they show poor resorbability and remain in the transplanted area even after 10 years. [1] Alternative therapeutics using growth factors (e.g., bone morphogenetic protein 2 and basic fibroblast growth factor), [3] or cells, such as osteoblasts or mesenchymal stem/progenitor cells (MSCs), have also been introduced. [4] These methods, however, are costly and time-consuming, because cell therapies usually involve the transplantation of mature osteoblasts, and osteoblastic differentiation requires ≈2-3 weeks. [5] Recent multidisciplinary approaches merging the knowledge in developmental biology with methods/techniques in tissue engineering have enabled the development of unprecedented technologies for cartilage and bone tissue engineering. [6] In this context, previous approaches for promoting bone regeneration were suboptimal as they were essentially based only on the concept of osteoblast-driven intramembranous ossification (IO).Bone is formed through two distinct processes: IO and endochondral ossification (EO). [7] Intramembranous ossification begins inside the osteoblast-secreted extracellular vesicles, i.e., matrix vesicles (MVs), and forms the flat bones (e.g., skull) and the cortical bone. [7][8][9] Matrix vesicles refer to small (20-200 nm) spherical bodies transported via lysosome and secreted by exocytosis from the cells, mainly osteoblasts. [10,11] On the other hand, in EO, bone replaces a cartilage intermediate. [12] Recently, EO was shown to initiate from chondrocyte-derived plasma membrane nanofragments (PMNFs), in the absence of osteoblasts and MVs. [8,13] Previous studies have shown that the membrane and enzymes constituting MVs are different from the parent cell membrane. [14,15] On the other hand, PMNFs are direct fragments of the parent cell membrane. Thus, MVs and PMNFs could be regarded to have different composition in phospholipids and enzymes, [14,16] and could be leading to the formation of apatite clusters with different structures. However, little is known about the exact morphology of bone apatite clusters formed from MVs (IO) and PMNFs (EO).

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Osteoclastogenic differentiation of human precursor cells over micro- and nanostructured hydroxyapatite topography

Cited by 25 publications

References 41 publications

Focus Ion Beam/Scanning Electron Microscopy Characterization of Osteoclastic Resorption of Calcium Phosphate Substrates

Focus Ion Beam/Scanning Electron Microscopy Characterization of Osteoclastic Resorption of Calcium Phosphate Substrates

Evaluation of a Porous Hydroxyapatite Granule and Gelatin Hydrogel Microsphere Composite in Bone Regeneration

Distinct Morphologies of Bone Apatite Clusters in Endochondral and Intramembranous Ossification

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