Osteoclastogenesis in peripheral blood mononuclear cell cultures of periprosthetic osteolysis patients and the phenotype of T cells localized in periprosthetic tissues

Roato, Ilaria; Caldo, Davide; D’Amico, Lucia; D’Amelio, Patrizia; Godio, Laura; Patanè, Salvatore; Astore, F.; Grappiolo, Guido; Boggio, Maurizio; Scagnelli, Roberto; Molfetta, Luigi; Ferracini, Riccardo

doi:10.1016/j.biomaterials.2010.06.027

Cited by 27 publications

(30 citation statements)

References 24 publications

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“…Importantly, metal wear particles were not observed in patient tissues to account for the presence of T cells. Although animal models have shown T cells are dispensable in the development of osteolysis [25,39,72], a recent in vitro study showed depletion of T cells or the addition of RANK-Fc to human peripheral blood cell cultures equally reduced osteoclast formation in response to RANKL [65]. Additionally, the current findings and those of others [28,61] support the potential involvement of T cells in human osteolytic responses after recruitment by chemotactic factors released from activated macrophages [5,19,63,68].…”

Section: Discussionsupporting

confidence: 65%

“…Specifically, T H cells play a major role in releasing cytokines (eg, RANKL) that promote macrophage differentiation into osteoclasts [10,23]. Although the role of T cells in aseptic loosening is controversial, a recent study has identified a functionally active subset of T C cells capable of downregulating T H cells [65], which may explain the inconsistent detection of lymphokines in tissues around loosened prostheses [45,65]. Additional studies showing correlations between increased numbers of T H and T C cells and radiographic osteolysis [28,61] further implicate T cell involvement in bone remodeling.…”

Section: Adaptive Immune Response Includes Several Subgroups Of T Lymmentioning

confidence: 99%

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Do Tissues From THA Revision of Highly Crosslinked UHMWPE Liners Contain Wear Debris and Associated Inflammation?

Baxter

Freeman

Kurtz

et al. 2011

Clinical Orthopaedics &Amp; Related Research

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Background Polyethylene wear debris is a major contributor to inflammation and the development of implant loosening, a leading cause of THA revisions. To reduce wear debris, highly crosslinked ultrahigh-molecular-weight polyethylene (UHMWPE) was introduced to improve wear properties of bearing surfaces. As highly crosslinked UHMWPE revision tissues are only now becoming available, it is possible to examine the presence and association of wear debris with inflammation in early implant loosening. Questions/purposes We asked: (1) Does the presence of UHMWPE wear debris in THA revision tissues correlate with innate and/or adaptive immune cell numbers? (2) Does the immune cell response differ between conventional and highly crosslinked UHMWPE cohorts?Methods We collected tissue samples from revision surgery of nine conventional and nine highly crosslinked UHMWPE liners. Polarized light microscopy was used to determine 0.5-to 2-lm UHMWPE particle number/mm 2 , and immunohistochemistry was performed to determine macrophage, T cell, and neutrophil number/mm 2 . Results For the conventional cohort, correlations were observed between wear debris and the magnitude of individual patient macrophage (q = 0.70) and T cell responses (q = 0.71) and between numbers of macrophages and T cells (q = 0.77) in periprosthetic tissues. In comparison, the highly crosslinked UHMWPE cohort showed a correlation between wear debris and the magnitude of macrophage responses (q = 0.57) and between macrophage and T cell numbers (q = 0.68). Although macrophages and T cells were present in both cohorts, the highly crosslinked UHMWPE cohort had lower numbers, which may be associated with shorter implantation times. Conclusions The presence of wear debris and inflammation in highly crosslinked UHMWPE revision tissues may contribute to early implant loosening.

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Section: Discussionsupporting

confidence: 65%

Section: Adaptive Immune Response Includes Several Subgroups Of T Lymmentioning

confidence: 99%

Do Tissues From THA Revision of Highly Crosslinked UHMWPE Liners Contain Wear Debris and Associated Inflammation?

Baxter

Freeman

Kurtz

et al. 2011

Clinical Orthopaedics &Amp; Related Research

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“…16 These data have been confirmed by our group in humans as well, during periprosthetic osteolysis. 17 Non-conventional T cells such as gd T cells have been suggested to have a pathogenetic role in a murine model of RA through the production of IL-17. 18 However, this role has not been confirmed in humans.…”

Section: Inflammatory Diseases Immune System and Bonementioning

confidence: 99%

“…This paper disagrees with previous reports showing a fundamental role of RANKL produced by T cells in OVX-induced bone loss. 1,5,17,36,47 In humans there are no convincing data on the role of B cells in postmenopausal osteoporosis. Nevertheless, changes in several B lymphocyte populations in patients affected by postmenopausal osteoporosis regardless of their estrogen status have been shown.…”

Section: Inflammatory Diseases Immune System and Bonementioning

confidence: 99%

Osteoimmunology: from mice to humans

Sassi¹

2016

Bonekey Reports

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The immune system has been recognized as one of the most important regulators of bone turnover and its deregulation is implicated in several bone diseases such as postmenopausal osteoporosis and inflammatory bone loss; recently it has been suggested that the gut microbiota may influence bone turnover by modulation of the immune system. The study of the relationship between the immune system and bone metabolism is generally indicated under the term 'osteoimmunology'. The vast majority of these studies have been performed in animal models; however, several data have been confirmed in humans as well: this review summarizes recent data on the relationship between the immune system and bone with particular regard to the data confirmed in humans.

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“…Periprosthetic osteolysis patients show T cell-dependent osteoclastogenesis in PBMC cultures; in fact the process was inhibited by RANK-Fc and T cell depletion [60] . In periprosthetic tissues local CD8+ T cells showed a regulatory phenotype, expressing CD25 and FoxP3, while CD4+ T cells did not express activation markers.…”

Section: Cytokines and Oc Formationmentioning

confidence: 99%