Osteoclast and Osteoblast Development of Mus musculus Haemopoietic Mononucleated Cells

Zarina, Z. A Intan; Hisham, Z. A. Shahrul; Rohaya, M. A. W.; Sahidan, S.; Ariffin, Shahrul Hisham Zainal

doi:10.3923/jbs.2008.506.516

Cited by 18 publications

(12 citation statements)

References 46 publications

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“…The procedures were done as approved by our institutional ethical committee (UKM 1.5.3.5/244/02-01-02-SF1052). Isolation of peripheral blood mononucleated cells were conducted as previously described with minor modifications (Ruzanna et al 2012 ; Shahrul Hisham et al 2008 ). Briefly, mice blood samples were diluted one times with 1X phosphate buffered saline; PBS (Sigma, St. Louis, MO, USA) and human blood samples were diluted three times with Hanks’ balanced salt solution; HBSS (Sigma).…”

Section: Methodsmentioning

confidence: 99%

“…Hematopoietic stem cells (HSCs) are adult stem cell that can differentiate into myeloid and lymphoid progenitors (Doulatov et al 2010 ). There are several sources of HSCs, namely cord blood (Broxmeyer et al 2011 ; Ferreira et al 2012 ), bone marrow (Fukata et al 2013 ; Schirhagl et al 2011 ) and peripheral blood (Muhammad Dain et al 2011 ; Ruzanna et al 2012 ; Shahrul Hisham et al 2008 ). It is relatively easier for the donor to donate peripheral blood as opposed to bone marrow as the means of harvesting bone marrow is typically painful and results in extended recovery time for the donor (Korbling 2001 ).…”

Section: Introductionmentioning

confidence: 99%

“…Currently, HSCs remain as the best characterized adult stem cells, with the studies stretching back to the 1960s (Armstrong et al 2012 ; Lacadie and Zon 2011 ). HSCs are shown to be able to differentiate into osteoclasts (Muhammad Dain et al 2011 ; Ruzanna et al 2012 ; Shahrul Hisham et al 2008 ), osteoblasts (Hofmann et al 2013 ; Muhammad Dain et al 2011 ; Ruzanna et al 2012 ; Shahrul Hisham et al 2008 ), dendritic cells (Hamdorf et al 2011 ), cardiomyocytes (Fukata et al 2013 ), chondrocytes (Ogawa et al 2010 ; Shahrul Hisham et al 2008 ), and fibroblasts and adipocytes (Ogawa et al 2010 ). It has previously proven that stem cells of both mesenchymal (adherent) and hematopoietic (suspension) origin are present in the population of peripheral blood mononucleated cells (Muhammad Dain et al 2011 ; Ruzanna et al 2012 ; Shahrul Hisham et al 2008 ).…”

Section: Introductionmentioning

confidence: 99%

“…In regards to suspension PBMNSCs, lack of research could be the reason behind insufficient data on the optimum culture medium. Some media combinations that have been used include RPMI1640 + 10 % fetal bovine serum (FBS) + 0.1 % penicillin–streptomycin (Potdar and Subedi 2011 ), α-MEM + 20 % FBS + 2 mM l -glutamine + 55 μM 2-mercaptoethanol + 100 U/ml penicillin + 100 μg/ml streptomycin (Akiyama et al 2012 ) and α-MEM + 10 % newborn calf serum (NBCS) + 1 % penicillin–streptomycin (Muhammad Dain et al 2011 ; Ruzanna et al 2012 ; Shahrul Hisham et al 2008 ). Thus, the focus of this study is to determine the best combinations of basal media and serum for the optimal proliferation of the stem cells.…”

Section: Introductionmentioning

confidence: 99%

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Analyses of basal media and serum for in vitro expansion of suspension peripheral blood mononucleated stem cell

et al. 2015

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Transplantation of stem cells requires a huge amount of cells, deeming the expansion of the cells in vitro necessary. The aim of this study is to define the optimal combination of basal medium and serum for the expansion of suspension peripheral blood mononucleated stem cells (PBMNSCs) without resulting in loss in the differentiation potential. Mononucleated cells were isolated from both mice and human peripheral blood samples through gradient centrifugation and expanded in α-MEM, RPMI, MEM or DMEM supplemented with either NBCS or FBS. The suspension cells were then differentiated to osteoblast. Our data suggested that α-MEM supplemented with 10 % (v/v) NBCS gives the highest fold increase after 14 days of culture for both mice and human PBMNSCs, which were ~1.51 and ~2.01 times, respectively. The suspension PBMNSCs in the respective medium were also able to maintain osteoblast differentiation potential as supported by the significant increase in ALP specific activity. The cells are also viable during the differentiated states when using this media. All these data strongly suggested that α-MEM supplemented with 10 % NBCS is the best media for the expansion of both mouse and human suspension PBMNSCs.

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Section: Methodsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

Analyses of basal media and serum for in vitro expansion of suspension peripheral blood mononucleated stem cell

et al. 2015

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“…ALP activity is found higher at tension sites compared with compression sites during orthodontic tooth movement [3]. The increasing osteoblast activities during bone formation will be characterized by the increasing ALP expressions [4].…”

Section: Introductionmentioning

confidence: 99%

Effect of carbonated hydroxyapatite incorporated advanced platelet rich fibrin intrasulcular injection on the alkaline phosphatase level during orthodontic relapse

Alhasyimi¹,

Pudyani²,

Asmara³

et al. 2018

AIP Conference Proceedings

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Stem Cell Heterogeneity of Mononucleated Cells from Murine Peripheral Blood: Molecular Analysis

Yazid

Ariffin

Senafi

et al. 2011

The Scientific World JOURNAL

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The main purpose of this paper was to determine the heterogeneity of primary isolated mononucleated cells that originated from the peripheral blood system by observing molecular markers. The isolated cells were cultured in complete medium for 4 to 7 days prior to the separation of different cell types, that is, adherent and suspension. Following a total culture time of 14 days, adherent cells activated the Cd105 gene while suspension cells activated the Sca-1 gene. Both progenitor markers, Cbfa-1 and Ostf-1, were inactivated in both suspension and adherent cells after 14-day culture compared to cells cultured 3 days in designated differentiation medium. In conclusion, molecular analyses showed that primary mononucleated cells are heterogeneous, consisting of hematopoietic stem cells (suspension) and mesenchymal stem cells (adherent) while both cells contained no progenitor cells.

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Osteoclast and Osteoblast Development of Mus musculus Haemopoietic Mononucleated Cells

Cited by 18 publications

References 46 publications

Analyses of basal media and serum for in vitro expansion of suspension peripheral blood mononucleated stem cell

Analyses of basal media and serum for in vitro expansion of suspension peripheral blood mononucleated stem cell

Effect of carbonated hydroxyapatite incorporated advanced platelet rich fibrin intrasulcular injection on the alkaline phosphatase level during orthodontic relapse

Stem Cell Heterogeneity of Mononucleated Cells from Murine Peripheral Blood: Molecular Analysis

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