Osteoblastic Differentiation of Wharton Jelly Biopsy Specimens and Their Mesenchymal Stromal Cells after Serum-Free Culture

Mueller, Andreas; Forraz, Nico; Gueven, Sinan; Atzeni, Gianluigi; Degoul, Olivier; Pagnon-Minot, Aurélie; Martin, Ivan; Scherberich, Arnaud; McGuckin, Colin

doi:10.1097/prs.0000000000000305

Cited by 15 publications

(9 citation statements)

References 27 publications

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“…No change in ALP staining pattern was observed between 14 and 21 days of culture. Although ALPL results are consistent with the previous published work [45], we hypothesis that the inter-individual responses of SCs due to heterogeneity and donor number (>6) are thought to be responsible for the absence of significant regulation of ALPL mRNA.…”

Section: Osteogenic Commitment Of Stem Cells On Capsupporting

confidence: 88%

Harnessing Wharton’s jelly stem cell differentiation into bone-like nodule on calcium phosphate substrate without osteoinductive factors

et al. 2017

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Designing materials to direct stem cell fate has a relevant impact on stem cell biology and provides insights facilitating their clinical application in regenerative medicine. Inspired by natural bone compositions, a friendly automated spray-assisted system was used to build calcium phosphate substrate (CaP). Sprayed biomimetic solutions using mild conditions led to the formation of CaP with controlled physical properties, good bioactivity and biocompatibility. Herein, we show that via optimization of physical properties, CaP substrate induce osteogenic differentiation of Wharton's jelly stem cells (WJ-SCs) without adding osteogenic supplement factors. These results suggest a great potential of sprayed CaP and WJ-SCs in bone tissue engineering and may facilitate the transition from bench to beside and the development of clinically successful engineered bone.

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Section: Osteogenic Commitment Of Stem Cells On Capsupporting

confidence: 88%

Harnessing Wharton’s jelly stem cell differentiation into bone-like nodule on calcium phosphate substrate without osteoinductive factors

et al. 2017

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“…Similarly, implanted UC-MSCs remained detectable in a skin lesion model only up to 11 days [44]. However, others have reported that ectopically implanted UC-MSCs could be detected in newly formed bone tissue after 8 weeks of implantation [45].…”

Section: Discussionmentioning

confidence: 99%

Transplanted Umbilical Cord Mesenchymal Stem Cells Modify the In Vivo Microenvironment Enhancing Angiogenesis and Leading to Bone Regeneration

Todeschi

Backly

Capelli

et al. 2015

Stem Cells and Development

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Umbilical cord mesenchymal stem cells (UC-MSCs) show properties similar to bone marrow mesenchymal stem cells (BM-MSCs), although controversial data exist regarding their osteogenic potential. We prepared clinical-grade UC-MSCs from Wharton's Jelly and we investigated if UC-MSCs could be used as substitutes for BM-MSCs in muscoloskeletal regeneration as a more readily available and functional source of MSCs. UCMSCs were loaded onto scaffolds and implanted subcutaneously (ectopically) and in critical-sized calvarial defects (orthotopically) in mice. For live cell-tracking experiments, UC-MSCs were first transduced with the luciferase gene. Angiogenic properties of UC-MSCs were tested using the mouse metatarsal angiogenesis assay. Cell secretomes were screened for the presence of various cytokines using an array assay. Analysis of implanted scaffolds showed that UC-MSCs, contrary to BM-MSCs, remained detectable in the implants for 3 weeks at most and did not induce bone formation in an ectopic location. Instead, they induced a significant increase of blood vessel ingrowth. In agreement with these observations, UC-MSC-conditioned medium presented a distinct and stronger proinflammatory/chemotactic cytokine profile than BM-MSCs and a significantly enhanced angiogenic activity. When UC-MSCs were orthotopically transplanted in a calvarial defect, they promoted increased bone formation as well as BM-MSCs. However, at variance with BM-MSCs, the new bone was deposited through the activity of stimulated host cells, highlighting the importance of the microenvironment on determining cell commitment and response. Therefore, we propose, as therapy for bone lesions, the use of allogeneic UC-MSCs by not depositing bone matrix directly, but acting through the activation of endogenous repair mechanisms.

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“… 235 , 236 , 237 , 238 They expressed typical MSC markers CD29, CD44, CD73, CD90, and CD105, but were negative for CD14, CD34, and CD45 235 , 236 , 237 , 238 ; with 98% to 99% of cells positive for these MSC after five passages, with the exception of CD105 which was positive in 75% of cells. 236 hUCSC have self‐renewing capability, leading to high proliferation and have also demonstrated multidifferentiation capacity, 235 , 239 differentiating into cells such as osteogenic, chondrogenic, and adipogenic cell lines, 240 , 241 , 242 , 243 including muscle cells, 244 neural cells, 245 and IVD cells. 235 , †††††††…”

Section: Umbilical Cord Stem Cellsmentioning

confidence: 99%

Cell sources proposed for nucleus pulposus regeneration

et al. 2021

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Lower back pain (LBP) occurs in 80% of adults in their lifetime; resulting in LBP being one of the biggest causes of disability worldwide. Chronic LBP has been linked to the degeneration of the intervertebral disc (IVD). The current treatments for chronic back pain only provide alleviation of symptoms through pain relief, tissue removal, or spinal fusion; none of which target regenerating the degenerate IVD. As nucleus pulposus (NP) degeneration is thought to represent a key initiation site of IVD degeneration, cell therapy that specifically targets the restoration of the NP has been reviewed here. A literature search to quantitatively assess all cell types used in NP regeneration was undertaken. With key cell sources: NP cells; annulus fibrosus cells; notochordal cells; chondrocytes; bone marrow mesenchymal stromal cells; adiposederived stromal cells; and induced pluripotent stem cells extensively analyzed for their regenerative potential of the NP. This review highlights: accessibility; expansion capability in vitro; cell survival in an IVD environment; regenerative potential; and safety for these key potential cell sources. In conclusion, while several potential cell sources have been proposed, iPSC may provide the most promising regenerative potential.

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Osteoblastic Differentiation of Wharton Jelly Biopsy Specimens and Their Mesenchymal Stromal Cells after Serum-Free Culture

Cited by 15 publications

References 27 publications

Harnessing Wharton’s jelly stem cell differentiation into bone-like nodule on calcium phosphate substrate without osteoinductive factors

Harnessing Wharton’s jelly stem cell differentiation into bone-like nodule on calcium phosphate substrate without osteoinductive factors

Transplanted Umbilical Cord Mesenchymal Stem Cells Modify the In Vivo Microenvironment Enhancing Angiogenesis and Leading to Bone Regeneration

Cell sources proposed for nucleus pulposus regeneration

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