Osteoblast Progenitors Enhance Osteogenic Differentiation of Periodontal Ligament Stem Cells

Yu, Miao; Wang, Limei; Ba, Pengfei; Li, Linxia; Sun, Long; Duan, Xiaoqi; Yang, Pishan; Yang, Chengzhe; Qi-zhong, Sun

doi:10.1902/jop.2017.170016

Cited by 15 publications

(12 citation statements)

References 32 publications

(59 reference statements)

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“…High RUNX2 expression was detected in preosteoblasts, immature osteoblasts, and early osteoblasts, which can be considered components of early differentiation[ 39 ]. OPN expression was observed at the middle/later stage of differentiation, and Col I represents the extracellular matrix during bone formation[ 40 , 41 ]. ALP is an early marker of osteogenic differentiation, and ALP activity is closely related to osteogenic differentiation[ 42 ].…”

Section: Discussionmentioning

confidence: 99%

Combined treatment with electrical stimulation and insulin-like growth factor-1 promotes bone regeneration in vitro

Xia

et al. 2018

PLoS ONE

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Electrical stimulation (ES) and insulin-like growth factor-1 (IGF-1) are widely used in bone regeneration because of their osteogenic activity. However, the combined effects of ES and supplemental IGF-1 on the whole bone formation process remain unclear. In this study, fluorescence staining and an MTT assay were first utilized to observe the influence of ES and IGF-1 on MC3T3-E1 cell proliferation and adhesion in vitro. Subsequently, osteogenic differentiation was evaluated by the alkaline phosphatase activity (ALP) and the expression of osteogenic marker genes. In addition, cell mineralization was determined by alizarin red staining and scanning electron microscopy (SEM). We demonstrated that the MC3T3-E1 cell proliferation was significantly higher for treatments combining IGF-1 and ES than for treatments with IGF-1 alone. The combination of IGF-1 and ES increased the MC3T3-E1 cell ALP activity, the expression of osteogenesis-related genes and the calcium deposition with a clear dose-dependent effect. Our data show the synergistic effect of IGF-1 and ES in promoting the proliferation, differentiation and mineralization of MC3T3-E1 cells, which suggests that it would be more effective to combine the proper dose of IGF-1 with ES to promote local bone damage repair and regeneration.

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Section: Discussionmentioning

confidence: 99%

Combined treatment with electrical stimulation and insulin-like growth factor-1 promotes bone regeneration in vitro

Xia

et al. 2018

PLoS ONE

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“…Thus, PDLSCs are regarded as critical factors in periodontal regeneration. 3 In the context of periodontal tissue engineering, PDLSC function is affected by periodontal microenvironment. Invasion by periodontal pathogens can directly damage periodontal tissues and activate host defense cells, which subsequently produce and secrete pro-inflammatory cytokines, (e.g., TNF-α) that contribute to the destruction of periodontal tissues.…”

Section: Discussionmentioning

confidence: 99%

“…The ecological imbalance between host cells and bacteria results in progressive destruction of periodontal tissue. 3,4 Dysregulation of the immune inflammatory response can cause hypersecretion of proinflammatory cytokines including tumor necrosis factor-a (TNF-a), a factor involved in tissue destruction in periodontitis. 5 Nagasawa et al 6 found elevated concentrations of TNF-a in gingival crevicular fluid from patients with periodontitis; the concentration of TNF-a was correlated with periodontitis activity.…”

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Progranulin promotes osteogenic differentiation of periodontal membrane stem cells in both inflammatory and non-inflammatory conditions

Sun

Ba³

et al. 2021

J Int Med Res

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Objective The growth factor progranulin (PGRN) is widely expressed and plays important roles in anti-inflammatory signaling and bone regeneration. However, the anti-inflammatory and pro-osteogenic roles of PGRN in periodontitis are seldom studied. We used an in vitro model to investigate whether PGRN can promote osteogenic differentiation of periodontal ligament stem cells (PDLSCs). Methods PDLSCs were treated with PGRN (0 to 100 ng/mL) and the optimal concentrations required to induce proliferation and osteogenesis were identified. PDLSCs were cultured with 10 ng/mL tumor necrosis factor (TNF)-α, 25 ng/mL PGRN, or 10 ng/mL TNF-α + 25 ng/ml PGRN; untreated PDLSCs were used as controls. The effects of PGRN on PDLSC proliferation and osteogenic differentiation were assessed. Results PGRN (5, 25, and 50 ng/mL) promoted PDLSC proliferation and osteogenic differentiation, with the 25-ng/mL dose showing the largest effect. Furthermore, 25 ng/mL PGRN reversed inhibition of osteogenic differentiation by TNF-α. Conclusion PGRN promotes PDLSC proliferation, osteogenic differentiation, and mineralization in both inflammatory and non-inflammatory conditions. The 25-ng/mL PRGN dose was the most suitable for inducing proliferation and osteogenesis. Further studies using animal models will be required to obtain pre-clinical evidence to support using PGRN as a treatment for periodontitis.

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“…Recently, it has been discovered that, in addition to their direct differentiation into tissue-related cell type, MSCs behave a potential paracrine effect, which includes anti-inflammatory and/or immunomodulation; antiapoptosis; angiogenesis, stimulating the growth and differentiation of surrounding cells [17]; and chemoattraction properties [18,19]. Moreover, some scholars even argued that instead of directly transforming into a tissue-related cell type, transplanted MSCs promote the proliferation and differentiation of autologous MSCs by regulating the microenvironment of the lesion site [20,21].…”

Section: Introductionmentioning

confidence: 99%

Conditioned medium derived from FGF-2-modified GMSCs enhances migration and angiogenesis of human umbilical vein endothelial cells

et al. 2020

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Background: Angiogenesis plays an important role in tissue repair and regeneration, and conditioned medium (CM) derived from mesenchymal stem cells (MSC-CM) possesses pro-angiogenesis. Nevertheless, the profile and concentration of growth factors in MSC-CM remain to be optimized. Fibroblast growth factor-2 (FGF-2) has been proven to be an effective angiogenic factor. Thus, the aim of this study was to verify whether FGF-2 gene overexpression optimized CM from human gingival mesenchymal stem cells (hGMSCs) and whether such optimized CM possessed more favorable pro-angiogenesis effect.Methods: First, FGF-2 gene-modified hGMSCs were constructed using lentiviral transfection technology (LV-FGF-2 + -hGMSCs) and the concentration of angiogenesis-related factors in LV-FGF-2 + -hGMSC-CM was determined by ELISA. Then, human umbilical vein endothelial cells (HUVECs) were co-cultured for 3 days with LV-FGF-2 + -hGMSC-CM, and the expression level of placenta growth factor (PLGF), stem cell factor (SCF), vascular endothelial growth factor receptor 2 (VEGFR2) in HUVECs were determined by qRT-PCR, western blot, and cellular immunofluorescence techniques. The migration assay using transwell and in vitro tube formation experiments on matrigel matrix was conducted to determine the chemotaxis and angiogenesis enhanced by LV-FGF-2 + -hGMSC-CM. Finally, NOD-SCID mice were injected with matrigel mixed LV-FGF-2 + -hGMSC-CM, and the plug sections were analyzed by immunohistochemistry staining with anti-human CD31 antibody.Results: LV-FGF-2 + -hGMSC-CM contained significantly more FGF-2, vascular endothelial growth factor A (VEGF-A), and transforming growth factor β (TGF-β) than hGMSC-CM. HUVECs pretreated with LV-FGF-2 + -hGMSC-CM expressed significantly more PLGF, SCF, and VEGFR2 at gene and protein level than hGMSC-CM pretreated HUVECs. Compared with hGMSC-CM, LV-FGF-2 + -hGMSC-CM presented significantly stronger chemotaxis to HUVECs and significantly strengthened HUVECs mediated in vitro tube formation ability. In vivo, LV-FGF-2 + -hGMSC-CM also possessed stronger promoting angiogenesis ability than hGMSC-CM.Conclusions: Overexpression of FGF-2 gene promotes hGMSCs paracrine of angiogenesis-related growth factors, thereby obtaining an optimized conditioned medium for angiogenesis promotion.

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Osteoblast Progenitors Enhance Osteogenic Differentiation of Periodontal Ligament Stem Cells

Abstract: Preosteoblasts can indirectly enhance the osteoblastic/cementoblastic differentiation and mineralization of PDLSCs with an optimal preosteoblasts:PDLSCs ratio in the range of 2:1 to 1:1.

Cited by 15 publications

References 32 publications

Combined treatment with electrical stimulation and insulin-like growth factor-1 promotes bone regeneration in vitro

Combined treatment with electrical stimulation and insulin-like growth factor-1 promotes bone regeneration in vitro

Progranulin promotes osteogenic differentiation of periodontal membrane stem cells in both inflammatory and non-inflammatory conditions

Conditioned medium derived from FGF-2-modified GMSCs enhances migration and angiogenesis of human umbilical vein endothelial cells

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