Combined treatment with electrical stimulation and insulin-like growth factor-1 promotes bone regeneration in vitro

Qi, Zhiping; Xia, Peng; S, Pan; Zheng, Shuang; Fu, Chen; Chang, Yuxin; Ma, Yue; Wang, Jincheng; Yang, Xiaoyu

doi:10.1371/journal.pone.0197006

Cited by 32 publications

(20 citation statements)

References 49 publications

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“…The previous studies have shown that alternating current (AC) and direct current (DC) are 2 kinds of electrical current which have been used in the field of tissue engineering . Alternating currents at different frequencies can guide the MSCs into osteogenic‐ and/or cardiac‐like cells at the specific conditions with the view to current frequency, substrate types and stem cell sources . However, there are not enough studies to evaluate the effect of alternating current on neural differentiation of stem cells.…”

Section: Discussionmentioning

confidence: 99%

“…61,62 Alternating currents at different frequencies can guide the MSCs into osteogenic-and/or cardiac-like cells at the specific conditions with the view to current frequency, substrate types and stem cell sources. 65,66 However, there are not enough studies to evaluate the effect of alternating current on neural differentiation of stem cells. As such, in this study the impact of AC currents at 0.1 frequency and 100-Hz frequency on MSCs fate were studied.…”

Section: Discussionmentioning

confidence: 99%

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Conductive electrospun scaffolds with electrical stimulation for neural differentiation of conjunctiva mesenchymal stem cells

et al. 2019

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An electrical stimulus is a new approach to neural differentiation of stem cells. In this work, the neural differentiation of conjunctiva mesenchymal stem cells (CJMSCs) on a new 3D conductive fibrous scaffold of silk fibroin (SF) and reduced graphene oxide (rGo) were examined. rGo (3.5% w/w) was dispersed in SF‐acid formic solution (10% w/v) and conductive nanofibrous scaffold was fabricated using the electrospinning method. SEM and TEM microscopies were used for fibrous scaffold characterization. CJMSCs were cultured on the scaffold and 2 electrical impulse models (Current 1:115 V/m, 100‐Hz frequency and current 2:115 v/m voltages, 0.1‐Hz frequency) were applied for 7 days. Also, the effect of the fibrous scaffold and electrical impulses on cell viability and neural gene expression were examined using MTT assay and qPCR analysis. Fibrous scaffold with the 220 ± 20 nm diameter and good dispersion of graphene nanosheets at the surface of nanofibers were fabricated. The MTT result showed the viability of cells on the scaffold, with current 2 lower than current 1. qPCR analysis confirmed that the expression of β‐tubulin (2.4‐fold P ≤ 0.026), MAP‐2 (1.48‐fold; P ≤ 0.03), and nestin (1.5‐fold; P ≤ 0.03) genes were higher in CJMSCs on conductive scaffold with 100‐Hz frequency compared to 0.1‐Hz frequency. Collectively, we proposed that SF‐rGo fibrous scaffolds, as a new conductive fibrous scaffold with electrical stimulation are good strategies for neural differentiation of stem cells and the type of electrical pulses has an influence on neural differentiation and proliferation of CJMSCs.

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Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Conductive electrospun scaffolds with electrical stimulation for neural differentiation of conjunctiva mesenchymal stem cells

et al. 2019

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“…The investigation of in vivo implantation of IGF1-coupled collagen membrane onto full-thickness articular cartilage defects have proven that cartilage repair and bone repair can be achieved by different dose of IGF1 [ 50 ]. All results have implied that IGF1 plays an important role in bone and cartilage formation, and also is vital for differentiation and metabolism [ [51] , [52] , [53] ]. CPS bioceramic individually adsorbed IGF1 in this work, in addition to favorable effects on bone and cartilage formation, IGF1 could bind cell surface IGF1 receptor to activate focal adhesion pathway and rap1 signaling pathway and thus promote cell adhesion.…”

Section: Discussionmentioning

confidence: 99%

Advanced protein adsorption properties of a novel silicate-based bioceramic: A proteomic analysis

Deng

Zhai

Yin

et al. 2021

Bioactive Materials

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Silicate bioceramics have been shown to possess excellent cytocompatibility and osteogenic activity, but the exact mechanism is still unclear. Protein adsorption is the first event taking place at the biomaterial-tissue interface, which is vital to the subsequent cellular behavior and further influence the biomaterial-tissue interaction. In this work, the protein adsorption behavior of a novel CPS bioceramic was evaluated using the proteomics technology. The results showed that CPS adsorbed more amount and types of serum proteins than HA. FN1 and IGF1 proteins selected from proteomics results were validated by Western-blot experiment. Pathway analysis also revealed mechanistic insights how these absorbed proteins by CPS help mediate cell adhesion and promotes osteogenic activity. Firstly, the dramatically enhanced adsorption of FN1 could greatly promote cell adhesion and growth. Secondly, IGF1 was uniquely adsorbed on CPS bioceramic and IGF1 could activate Rap1 signaling pathway to promote cell adhesion. Thirdly, the increased adsorption of FN1, IGF1 and COL1A2 proteins on CPS explains its better ability on bone regeneration than HA. Fourthly, the increased adsorption of IGF1, CHAD, COL2A1 and THBS4 proteins on CPS explains its ability on cartilage formation. Lastly, the increased adsorption of immunological related proteins on CPS may also play a positive role in bone regeneration. In addition, CPS had a much better cell adhesion ability than HA, proving that more adsorbed proteins really had a positive effect on cell behavior. The more adsorbed proteins on CPS than HA might indicated a better bone regeneration rate at early stage of implantation.

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“…Fiber Matrices. In our previous study, we have examined cell proliferation under ES with different frequencies and found that the 200 Hz group had the maximum cell proliferation compared to those of the other groups [41]. Therefore, we choose the frequency of 200 Hz for the subsequent cell experiments.…”

Section: Cell Morphology and Proliferation On Different Hybridmentioning

confidence: 99%

Enhanced Cell Proliferation and Osteogenesis Differentiation through a Combined Treatment of Poly-L-Lysine-Coated PLGA/Graphene Oxide Hybrid Fiber Matrices and Electrical Stimulation

Zhu

Zheng

et al. 2020

Journal of Nanomaterials

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Bone tissue engineering scaffold provides an effective treatment for bone defect repair. Biodegradable bone scaffold made of various synthetic and natural materials can be used as bone substitutes and grafts for defect site, which has great potential to support bone regeneration. Regulation of cell-scaffold material interactions is an important factor for modulating the cellular activity in bone tissue engineering scaffold applications. Thus, the hydrophilic, mechanical, and chemical properties of scaffold materials directly affect the results of bone regeneration and functional recovery. In this study, a poly-L-lysine (PLL) surface-modified poly(lactic-co-glycolic acid) (PLGA)/graphene oxide (GO) (PLL-PLGA/GO) hybrid fiber matrix was fabricated for bone tissue regeneration. Characterization of the resultant hybrid fiber matrices was done using scanning electron microscopy (SEM), contact angle, and a material testing machine. According to the results obtained from the test above, the PLL-PLGA/GO hybrid fiber matrices exhibited high wettability and mechanical strength. The special surface characteristics of PLL-PLGA/GO hybrid fiber matrices were more beneficial for protein adsorption and inhibit the proliferation of pathogens. Moreover, the enhanced regulation of MC3T3-E1 cell proliferation and differentiation was observed, when the resultant hybrid fiber matrices were combined with electrical stimulation (ES). The cellular response of MC3T3-E1 cells including cell adhesion, proliferation, alkaline phosphatase (ALP) activity, calcium deposition, and osteogenesis-related gene expression was significantly enhanced with the synergistic effect of resultant hybrid fiber matrices and ES. These data indicate that the PLL-PLGA/GO hybrid fiber matrices supported the cellular response in terms of cell proliferation and osteogenesis differentiation in the presence of electrical stimulation, which could be a potential treatment for bone defect.

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Combined treatment with electrical stimulation and insulin-like growth factor-1 promotes bone regeneration in vitro

Cited by 32 publications

References 49 publications

Conductive electrospun scaffolds with electrical stimulation for neural differentiation of conjunctiva mesenchymal stem cells

Conductive electrospun scaffolds with electrical stimulation for neural differentiation of conjunctiva mesenchymal stem cells

Advanced protein adsorption properties of a novel silicate-based bioceramic: A proteomic analysis

Enhanced Cell Proliferation and Osteogenesis Differentiation through a Combined Treatment of Poly-L-Lysine-Coated PLGA/Graphene Oxide Hybrid Fiber Matrices and Electrical Stimulation

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