Osteoblast Cell Response to Naturally Derived Calcium Phosphate-Based Materials

Abstract

The demand of calcium phosphate bioceramics for biomedical applications is constantly increasing. Efficient and cost-effective production can be achieved using naturally derived materials. In this work, calcium phosphate powders, obtained from dolomitic marble and Mytilus galloprovincialis seashells by a previously reported and improved Rathje method were used to fabricate microporous pellets through cold isostatic pressing followed by sintering at 1200 °C. The interaction of the developed materials with MC3T3-E1 pre-osteoblasts was explored in terms of cell adhesion, morphology, viability, proliferation, and differentiation to evaluate their potential for bone regeneration. Results showed appropriate cell adhesion and high viability without distinguishable differences in the morphological features. Likewise, the pre-osteoblast proliferation overtime on both naturally derived calcium phosphate materials showed a statistically significant increase comparable to that of commercial hydroxyapatite, used as reference material. Furthermore, evaluation of the intracellular alkaline phosphatase activity and collagen synthesis and deposition, used as markers of the osteogenic ability of these bioceramics, revealed that all samples promoted pre-osteoblast differentiation. However, a seashell-derived ceramic demonstrated a higher efficacy in inducing cell differentiation, almost equivalent to that of the commercial hydroxyapatite. Therefore, data obtained demonstrate that this naturally sourced calcium-phosphate material holds promise for applications in bone tissue regeneration.

Synthesis of Conductive Carbon Aerogels Decorated with β-Tricalcium Phosphate Nanocrystallites

“…The positive observation from the cellular behavior here was suggested to be due to the presence of the β-TCP found in the carbon fibers. Previous literature on the potential of the β-TCP for osteoblasts culture and bone remodeling through its chemical and physical properties support this suggestion 43,44 . Therefore, the developed ceramic nanocrystallite decorated surface improves cellular behavior.…”

Hydroxyapatite based biocomposite scaffold: A highly biocompatible material for bone regeneration

“…On day four, live cells layer can be seen from all culture groups, which shows that the biocomposite and Sr/Mg-HA nanoparticles have no negative impact on biocompatibility. Additionally, the fluorescence pictures likewise display numerous filopodia, exhibiting admirable cells connection on the biocomposite ( Mitran et al, 2018 ).…”

Influence of preferred orientation on the bioactivity of hydroxyapatite: a potential tooth repair and implant surface coating material

“…2b and 2c revealed HApS1 and HApS2 to be carbonated materials. Carbonated HAp has been reported to possess some similarity with bone apatite in terms of structural composition [6] and is one of the indicators of HAp with comparable bone apatite resemblance. The difference in the FTIR spectra was due to the unique OH band, which characterized the structure of HApS2 as compared to HApS1.…”

“…Scientific literature reports that substitution of ions like zinc, magnesium, copper, phosphite, and carbonate into HAp results in the formation of HAp with a comparable natural bone resemblance (in terms of composition, structure, and morphology). This thus enhances its bioactivity for a rapid osteointegration instigation [6]. Additionally, the bioactivity results in the formation of a calcium phosphate…”