Osseointegration of titanium dental implant under fluoride exposure in rabbits: Micro‐CT and histomorphometry study

Abstract

Objective: This study aims to investigate the influence of fluoride exposure on implant osseointegration.Methods: A total of 24 male New Zealand white rabbits were randomly divided into the control group and the fluoride exposure group. Rabbits in the control group were fed with tap water, while those in the fluoride exposure group were given 200 mg/L sodium fluoride solution. After 2-month feeding, implants were inserted into the extraction socket immediately after extraction of rabbit mandibular anterior teeth.Four rabbits in each group were sacrificed to collect the implants samples at 1, 2, and 3 months post-implantation, respectively. Radiographic and histomorphometry examinations were performed to evaluate the condition of implant osseointegration.Results: Bone volume around the implants increased in a time-dependent manner in both groups. Micro-CT images illustrated that the bone mineral density (BMD) in the fluoride exposure group was significantly lower than that in the control group after implantation for 2 and 3 months. The bone-implant contact ratio (BIC) in the fluoride exposure group was much lower than that of the control group at 3 months post-implantation according to histomorphometry examination. Conclusions:In rabbit animal model, high fluoride exposure affected the quality of bone surrounding the implant and significantly reduced bone integration of the implant, especially in the late stage of osseointegration. K E Y W O R D Sfluoride exposure, implant osseointegration, peri-implant bone, rabbit

An In Vivo Study in Rat Femurs of Bioactive Silicate Coatings on Titanium Dental Implants

“…Previous studies have evaluated implant osseointegration using micro-CT imaging with encouraging results [31][32][33][34][35].…”

“…Animal studies on rats' femurs are considered valid models for assessing implant osseointegration [29], usually measuring the parameter "bone-to-implant contact" (BIC) on histological images/sections [30]. The bone-implant interface can also be investigated with micro-computed tomography (micro-CT), which enables the 3D evaluation of the bone surrounding the implants and does not preclude further assessments afterwards [31][32][33][34][35]. This method overcomes the limits of histological analysis, such as the fact that a single histological section may not be representative of the bone apposition over the whole implant [36], and the fact that it is hard to obtain serial sectioning of bony specimens containing little metal implants positioned in small animals such as mice [37][38][39] and rats [40,41].…”

A novel surgical model for the preclinical assessment of the osseointegration of dental implants: a surgical protocol and pilot study results

“…The surgical site was easily accessible through the conventional intraoral approach. There was minimal blood loss and low morbidity compared to other dental implant models as no extraction of primary teeth, lateralization of the inferior alveolar nerves or excessive bone removal were required [ 16 , 35 – 42 ]. The presented model was designed considering the three fundamental principles of animal research; replacement, reduction and refinement.…”

“…However, in many of these models the implants were placed in extraoral locations. Dental implants have been placed in the extraction sockets of the rabbit mandibular teeth by a handful of authors [ 16 , 37 , 39 – 42 ]. Munhoz et al in 2017 evaluated the impact of xenograft (Gen-Ox) on the osseointegration of dental implants after the extraction of the lower incisors bilaterally [ 16 ].…”

Effects of Zinc Ions Released from Ti-NW-Zn Surface on Osteogenesis and Angiogenesis in Vitro and in an in Vivo Zebrafish Model

“…In vivo experimental models especially rabbits and dogs have been used to simulate the oral implantation process and investigate the characteristics and the basic molecular mechanisms of osseointegration [15][16][17]. However, the complexity of metabolic and physical-chemical regulations of higher vertebrate makes it di cult to dissect the early phase of vascularized bone regeneration within a short period under zinc ions exposure.…”