OsRGLP2 promoter derived GUS expression in transgenic tobacco in response to salicylic acid, H2O2, PEG, NaCl and auxins

Ayub, Sonia; Hayat, Rabbia; Zainab, Zainab; Akhtar, Wasim; Mahmood, Tariq

doi:10.1016/j.plgene.2019.100190

Cited by 6 publications

(3 citation statements)

References 69 publications

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“…In the flowering stage, leaves that previously showed GUS activity ( Fig 4A.3 ), almost underwent ceased GUS activity in transgenic SBP-GUS ( Fig 4B.3 ). These decreased changes in gene expression in the same tissue were reported in OsRGLP2 expression in tobacco [ 54 ]. However, GUS activity was identified in reproductive organs, including sepal ( Fig 4C.1 ), stamen ( Fig 4D.3 ), petal ( Fig 4E.3 ), and immature seeds in SBP-GUS lines ( Fig 4F.3 ).…”

Section: Resultssupporting

confidence: 73%

Characterization of sucrose binding protein as a seed-specific promoter in transgenic tobacco Nicotiana tabacum L.

et al. 2022

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Seed-specific expression using appropriate promoters is a recommended strategy for the efficiently producing valuable metabolites in transgenic plants. In the present study, we investigated the sequence of sucrose binding protein (SBP) as a seed-specific promoter to find the cis-acting elements specific to gene expression in seeds. The 1860 bp SBP sequence was analyzed using Plant Care and PLACE databases to find cis-acting elements, which resulted in a finding of 22 cis-acting elements required for seed expression. In addition, we have discovered cis- acting elements that are indirectly involved in triacylglycerol synthesis (GATABOX, DOFCOREZM, CACGTGMOTIF). The seed specificity of SBP was analyzed by generating a stable transgenic tobacco plant harboring β-glucuronidase (GUS) reporter gene under the control of the SBP promoter. Histochemical analysis of these transgenic tobacco plants indicated decreasing GUS activity in the leaves during the vegetative stage. However, the mature seeds of transgenic plants showed GUS activity. Moreover, the SBP promoter function in the seed oil content was evaluated by the expression of DGAT1. The expression analysis of DGAT1 in SBP-DGAT1 transgenic tobacco seeds using quantitative real-time PCR revealed a 7.8-fold increase in DGAT1 than in non-transgenic plants. Moreover, oil content increased up to 2.19 times more than in non-transgenic plants. And the oil content of the SBP-DGAT1 transgenic tobacco leaves did not change compared to the control plant. Therefore, we suggested that the SBP promoter could be used as a seed-specific promoter for targeted expression of desired genes in the metabolite engineering of oilseed crops.

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Section: Resultssupporting

confidence: 73%

Characterization of sucrose binding protein as a seed-specific promoter in transgenic tobacco Nicotiana tabacum L.

et al. 2022

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“…Germin was first identified in wheat's embryos and labeled as a germination marker; it was subsequently characterized as a homohexamer glycoprotein with oxalate oxidase (OXO, EC 1.2.3.4) activity [1,2]. Proteins with similar amino acid sequences and structures to wheat germin are defined as germin-like proteins (GLPs) [3][4][5]. GLPs are a type of soluble glycoproteins with a "cupin" domain (PF00190) belonging to the "cupin superfamily" ubiquitously present in plants [6,7].…”

Section: Introductionmentioning

confidence: 99%

Characterization of Germin-like Proteins (GLPs) and Their Expression in Response to Abiotic and Biotic Stresses in Cucumber

Liao

Liu

et al. 2021

Horticulturae

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Germins and germin-like proteins (GLPs) are glycoproteins closely associated with plant development and stress response in the plant kingdom. Here, we carried out genome-wide identification and expression analysis of the GLP gene family in cucumber to study their possible functions. A total of 38 GLP genes were identified in cucumber, which could be mapped to six out of the seven cucumber chromosomes. A phylogenetic analysis of the GLP members from cucumber, Arabidopsis and rice showed that these GLPs could be divided into six groups, and cucumber GLPs in the same group had highly similar conserved motif distribution and gene structure. Gene duplication analysis revealed that six cucumber GLP genes were located in the segmental duplication regions of cucumber chromosomes, while 14 genes were associated with tandem duplications. Tissue expression profiles of cucumber GLP genes showed that many genes were preferentially expressed in specific tissues. In addition, some cucumber GLP genes were differentially expressed under salt, drought and ABA treatments, as well as under DM inoculation. Our results provide important information for the functional identification of GLP genes in the growth, development and stress response of cucumber.

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“…Attractively, the 35S:AcFT4 plants that do not bulb have a similar presence to leeks and sustain budding vegetatively into the winter, despite being deprived of their vegetation dying off. This recommends that the large phenotypic alterations between onion and leek might be due to an uncertain genetic variation [236][237][238][239][240][241]. Although the occurrence of enlarged bulbs is a distinct feature of onions, most members of the Allium genus produce some kind of storage organ.…”

Section: Ft Gene Regulates Bulb Formationmentioning

confidence: 99%

Mechanism of Allium Crops Bulb Enlargement in Response to Photoperiod: A Review

Atif

Ahanger

Amin

et al. 2020

IJMS

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The photoperiod marks a varied set of behaviors in plants, including bulbing. Bulbing is controlled by inner signals, which can be stimulated or subdued by the ecological environment. It had been broadly stated that phytohormones control the plant development, and they are considered to play a significant part in the bulb formation. The past decade has witnessed significant progress in understanding and advancement about the photoperiodic initiation of bulbing in plants. A noticeable query is to what degree the mechanisms discovered in bulb crops are also shared by other species and what other qualities are also dependent on photoperiod. The FLOWERING LOCUS T (FT) protein has a role in flowering; however, the FT genes were afterward reported to play further functions in other biological developments (e.g., bulbing). This is predominantly applicable in photoperiodic regulation, where the FT genes seem to have experienced significant development at the practical level and play a novel part in the switch of bulb formation in Alliums. The neofunctionalization of FT homologs in the photoperiodic environments detects these proteins as a new class of primary signaling mechanisms that control the growth and organogenesis in these agronomic-related species. In the present review, we report the underlying mechanisms regulating the photoperiodic-mediated bulb enlargement in Allium species. Therefore, the present review aims to systematically review the published literature on the bulbing mechanism of Allium crops in response to photoperiod. We also provide evidence showing that the bulbing transitions are controlled by phytohormones signaling and FT-like paralogues that respond to independent environmental cues (photoperiod), and we also show that an autorelay mechanism involving FT modulates the expression of the bulbing-control gene. Although a large number of studies have been conducted, several limitations and research gaps have been identified that need to be addressed in future studies.

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OsRGLP2 promoter derived GUS expression in transgenic tobacco in response to salicylic acid, H2O2, PEG, NaCl and auxins

Cited by 6 publications

References 69 publications

Characterization of sucrose binding protein as a seed-specific promoter in transgenic tobacco Nicotiana tabacum L.

Characterization of sucrose binding protein as a seed-specific promoter in transgenic tobacco Nicotiana tabacum L.

Characterization of Germin-like Proteins (GLPs) and Their Expression in Response to Abiotic and Biotic Stresses in Cucumber

Mechanism of Allium Crops Bulb Enlargement in Response to Photoperiod: A Review

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