OsMSR3, a Small Heat Shock Protein, Confers Enhanced Tolerance to Copper Stress in Arabidopsis thaliana

Cui, Yanchun; Wang, Manling; Yin, Xuming; Xu, Guoyun; Song, Shufeng; Li, Mingjuan; Li, Kai; Xia, X. Y.

doi:10.3390/ijms20236096

Cited by 34 publications

(18 citation statements)

References 59 publications

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“…In our proteomic study, 15 out of 20 HSPs in GE were down-regulated after 12 days of cold treatment, whereas the expression of four (XP_008448853.1, XP_008448861.1, XP_008463154.1, XP_008463443.1) out of 25 HSPs in JS remained high. After 24 days, 19 HSPs were down-regulated in GE, while 19 were upregulated in JS, which supports the idea that continuous high levels of HSPs may help plants adapt to stress stimuli [45]. DnaJ protein and Chaperonin have also been reported to protect plants against biotic and abiotic stress [46,47].…”

Section: Structural Proteinssupporting

confidence: 67%

iTRAQ-based quantitative proteomics analysis of cantaloupe (Cucumis melo var. saccharinus) after cold storage

et al. 2020

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Background: Cantaloupe is susceptible to cold stress when it is stored at low temperatures, resulting in the loss of edible and commercial quality. To ascertain the molecular mechanisms of low temperatures resistance in cantaloupe, a cold-sensitive cultivar, Golden Empress-308 (GE) and a cold-tolerant cultivar, Jia Shi-310 (JS), were selected in parallel for iTRAQ quantitative proteomic analysis. Results: The two kinds of commercial cultivars were exposed to a temperature of 0.5°C for 0, 12 and 24 days. We found that the cold-sensitive cultivar (GE) suffered more severe damage as the length of the cold treatment increased. Proteomic analysis of both cultivars indicated that the number of differentially expressed proteins (DEPs) changed remarkably during the chilly treatment. JS expressed cold-responsive proteins more rapidly and mobilized more groups of proteins than GE. Furthermore, metabolic analysis revealed that more amino acids were up-regulated in JS during the early phases of low temperatures stress. The DEPs we found were mainly related to carbohydrate and energy metabolism, structural proteins, reactive oxygen species scavenging, amino acids metabolism and signal transduction. The consequences of phenotype assays, metabolic analysis and q-PCR validation confirm the findings of the iTRAQ analysis. Conclusion: We found that the prompt response and mobilization of proteins in JS allowed it to maintain a higher level of cold tolerance than GE, and that the slower cold responses in GE may be a vital reason for the severe chilling injury commonly found in this cultivar. The candidate proteins we identified will form the basis of future studies and may improve our understanding of the mechanisms of cold tolerance in cantaloupe.

show abstract

Section: Structural Proteinssupporting

confidence: 67%

iTRAQ-based quantitative proteomics analysis of cantaloupe (Cucumis melo var. saccharinus) after cold storage

et al. 2020

View full text Add to dashboard Cite

show abstract

“…In our proteomic study, 15 out of 20 HSPs in GE were down-regulated after 12 days of cold treatment, whereas the expression of four (XP_008448853.1, XP_008448861.1, XP_008463154.1, XP_008463443.1) out of 25 HSPs in JS remained high. After 24 days, 19 HSPs were down-regulated in GE, while 19 were up-regulated in JS, which supports the idea that continuous high levels of HSPs may help plants adapt to stress stimuli [45]. DnaJ protein and Chaperonin have also been reported to protect plants against biotic and abiotic stress [46,47].…”

Section: Structural Proteinssupporting

confidence: 67%

iTRAQ-based quantitative proteomics analysis of cantaloupe (Cucumis melo var. saccharinus) after cold storage

Song

Tang

Cai

et al. 2020

Preprint

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Background: Cantaloupe is susceptible to cold stress when it is stored at low temperatures, resulting in the loss of edible and commercial quality. To ascertain the molecular mechanisms of low temperatures resistance in cantaloupe, a cold-sensitive cultivar, Golden Empress-308 (GE) and a cold-tolerant cultivar, Jia Shi-310 (JS), were selected in parallel for iTRAQ quantitative proteomic analysis. Results: The two kinds of commercial cultivars were exposed to a temperature of 0.5℃ for 0, 12 and 24 days. We found that the cold-sensitive cultivar (GE) suffered more severe damage as the length of the cold treatment increased. Proteomic analysis of both cultivars indicated that the number of differentially expressed proteins (DEPs) changed remarkably during the chilly treatment. JS expressed cold-responsive proteins more rapidly and mobilized more groups of proteins than GE. Furthermore, metabolic analysis revealed that more amino acids were up-regulated in JS during the early phases of low temperatures stress. The DEPs we found were mainly related to carbohydrate and energy metabolism, structural proteins, reactive oxygen species scavenging, amino acids metabolism and signal transduction. The consequences of phenotype assays, metabolic analysis and q-PCR validation confirm the findings of the iTRAQ analysis. Conclusion: We found that the prompt response and mobilization of proteins in JS allowed it to maintain a higher level of cold tolerance than GE, and that the slower cold responses in GE may be a vital reason for the severe chilling injury commonly found in this cultivar. The candidate proteins we identified will form the basis of future studies and may improve our understanding of the mechanisms of cold tolerance in cantaloupe.

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“…AtHsfA2 not only performs an essential function in heat and osmotic stress response but also exert an important part in the growth and development of plants (Charng et al 2007;Liu and Charng 2013; Ogawa et al 2007). OsMSR3, coding a small heat shock protein, confers enhanced tolerance to cadmium and copper stresses in Arabidopsis (Cui et al 2019). GhHsp24.7, a mitochondrial small heat shock protein in cotton regulates seed germination by inducing reactive oxygen species (ROS) via thermal sensing (Ma et al 2019).…”

Section: Introductionmentioning

confidence: 99%

Genome-Wide Identification and Expression Analysis of Hsf and Hsp Gene Families in Cucumber (Cucumis Sativus L.)

Chen

Wang

Tang

et al. 2021

Preprint

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Heat shock proteins (Hsps) are molecular chaperones that participate in plants' response to environmental stresses, including heat stress, and also play an essential role in plant growth and development. Hsps expression is monitored and regulated by specific types of transcription factors known as heat shock factors (Hsfs). Although the role of Hsfs and Hsps in stress response has been investigated in some plants, their role is still poorly understood in cucumber (Cucumis sativus L.). To reveal the mechanisms of cucumber Hsf and Hsp coping with various stresses, the analyses of cucumber Hsf and Hsp gene families were conducted using bioinformatics-based methods. A total of 23 Hsfs and 72 Hsps were identified in the cucumber genome (v3.0), and the gene structure and motif composition are relatively conserved in each subfamily. At least 23 pairs of heat shock genes underwent gene duplication in cucumber. A cis-element analysis is implicit that CsHsfs and CsHsps possessed at least one hormone or stress response cis-element, suggesting that CsHsf and CsHsp genes could respond to different stress conditions. Heatmaps of the CsHsf and CsHsp gene families indicated that most heat shock genes were expressed in different tissues and organs. RNA-seq showed that most of the cucumber Hsf and Hsp genes are differentially expressed upon exposure to biotic and abiotic stresses. These results provide valuable information to clarify the evolutionary relationship between the CsHsf and CsHsp family and to facilitate the functional characterization of the CsHsf and CsHsp genes in future studies.

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OsMSR3, a Small Heat Shock Protein, Confers Enhanced Tolerance to Copper Stress in Arabidopsis thaliana

Cited by 34 publications

References 59 publications

iTRAQ-based quantitative proteomics analysis of cantaloupe (Cucumis melo var. saccharinus) after cold storage

iTRAQ-based quantitative proteomics analysis of cantaloupe (Cucumis melo var. saccharinus) after cold storage

iTRAQ-based quantitative proteomics analysis of cantaloupe (Cucumis melo var. saccharinus) after cold storage

Genome-Wide Identification and Expression Analysis of Hsf and Hsp Gene Families in Cucumber (Cucumis Sativus L.)

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