iTRAQ-based quantitative proteomics analysis of cantaloupe (Cucumis melo var. saccharinus) after cold storage

Abstract

Background: Cantaloupe is susceptible to cold stress when it is stored at low temperatures, resulting in the loss of edible and commercial quality. To ascertain the molecular mechanisms of low temperatures resistance in cantaloupe, a cold-sensitive cultivar, Golden Empress-308 (GE) and a cold-tolerant cultivar, Jia Shi-310 (JS), were selected in parallel for iTRAQ quantitative proteomic analysis. Results: The two kinds of commercial cultivars were exposed to a temperature of 0.5°C for 0, 12 and 24 days. We found that the cold-sensitive cultivar (GE) suffered more severe damage as the length of the cold treatment increased. Proteomic analysis of both cultivars indicated that the number of differentially expressed proteins (DEPs) changed remarkably during the chilly treatment. JS expressed cold-responsive proteins more rapidly and mobilized more groups of proteins than GE. Furthermore, metabolic analysis revealed that more amino acids were up-regulated in JS during the early phases of low temperatures stress. The DEPs we found were mainly related to carbohydrate and energy metabolism, structural proteins, reactive oxygen species scavenging, amino acids metabolism and signal transduction. The consequences of phenotype assays, metabolic analysis and q-PCR validation confirm the findings of the iTRAQ analysis. Conclusion: We found that the prompt response and mobilization of proteins in JS allowed it to maintain a higher level of cold tolerance than GE, and that the slower cold responses in GE may be a vital reason for the severe chilling injury commonly found in this cultivar. The candidate proteins we identified will form the basis of future studies and may improve our understanding of the mechanisms of cold tolerance in cantaloupe.

COE Inhibits Vasculogenic Mimicry by Targeting EphA2 in Hepatocellular Carcinoma, a Research Based on Proteomics Analysis

“…Protein data analysis was performed according to previous description ( Song et al, 2020 ). Briefly, the raw data were converted into mgf files via Proteome Discoverer 1.4 (Thermo, American), the mgf data were further analyzed with Protein Pilot 5.0 (AB Sciex, United States).…”

Identification of Glutathione S-Transferase Genes in Hami Melon (Cucumis melo var. saccharinus) and Their Expression Analysis Under Cold Stress

“…Gas chromatography/mass spectrometry (GC/MS) was optimized for amino acids as previously described ( Song et al, 2020 ). Raw data analysis was finished with Chroma TOF (V 4.3x,LECO) software, and the LECO-Fiehn Rtx5 database was utilized for metabolite identification by matching the mass spectrum and retention index ( Shah et al, 2020 ).…”

“…Based on our previous study, we find that GSTs are positively expressed in cold-tolerant Hami melon (JS) and are regarded as candidate cold-resistance proteins ( Song et al, 2020 ). Herein, a comprehensive study was carried out to identify the Hami melon GST gene family on the aspect of bioinformatics analysis, including molecular information, distribution on chromosomes, phylogenetic relationships, synteny relationships, gene structures, protein–protein interactions (PPIs), 3-D models, functional annotation and expression levels of total enzyme activities, and genes in response to cold stress.…”

Integrative Omics Analysis of Three Oil Palm Varieties Reveals (Tanzania × Ekona) TE as a Cold-Resistant Variety in Response to Low-Temperature Stress

“…Our results showed that glutathione S-transferase F11 (GSTF11) was upregulated in both OG and TE, while glutathione S-transferase (DHAR3) and (DHAR2) were upregulated in OG only. Glutathione S-transferase DHAR3 and glutathione S-transferase DHAR2-like proteins were upregulated in cantaloupe crops in response to cold stress [ 65 ]. Nonetheless, as antioxidant agents, peroxiredoxin (Prx) and thioredoxin play a vital role in redox signaling [ 61 , 66 ].…”