Osmotic properties of spermatozoa from felids producing different proportions of pleiomorphisms: influence of adding and removing cryoprotectant

Pukazhenthi, Budhan S.; Spindler, Rebecca; Wildt, David E.; Bush, Lena May; Howard, Jeremy

doi:10.1016/s0011-2240(02)00035-4

Cited by 43 publications

(38 citation statements)

References 41 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Lengwinat and Blottner (1994) reported a decreased motility and decreased acrosomal integrity after thawing. Therefore, cooled and cryopreserved sperms undergo severe damages that alter their motility and morphology, thus affecting their fertilizing potential (Watson, 1995;Pukazhenthi et al, 2002). This finding was also demonstrated in our present study using epididymal spermatozoa, the motility of spermatozoa was well maintained throughout the dilution procedure (83.3 ± 1.1, 80 ± 1.3, and 80±1.3% for collection, NSF-1, and NSF-2 groups respectively); but declined (P<0.05) after freezing and thawing (38.3 ± 3.1%) (Figure 1).…”

Section: Resultsmentioning

confidence: 99%

Characteristic of Frozen-Thawed Epididymal Spermatozoa and Refrigerated Storage of Ram Spermatozoa

Karja¹,

Respaty²,

Nuraini³

et al. 2010

J. Indonesian Trop. Anim. Agric.

View full text Add to dashboard Cite

Post-mortem spermatozoa recovery is an important technique for obtaining germplasm reserves from genetically valuable animals or endangered species. The purpose of this study was to study whether ram spermatozoa within epididymides stored at 4º C for 24 and 48 h remain their motility and viability. The characteristic of ram epididymal spermatozoa after freezing and thawing was also observed. Six pairs of ram testes with attached epididymides were used in this study. The motility of control spermatozoa was well maintained throughout the dilution procedure (83.3±1.1, 80±1.3, and 80±1.3% for collection, Niwa and Sasaki freezing -1 extender (NSF-1 and NSF-2 groups respectively); but declined (P<0.05) after freezing and thawing (38.3±3.1%). Motile and viable spermatozoa could be recovered from epididymides up to 48 h of storage, although their quality declined significantly (P<0.05) as post-mortem storage time increased (motility: 83±1.2, 67±3.0, and 46±5.1; viability: 84.2±2.4, 73±2.8, and 66.6±2.6 % for control, 24 h and 48 h group respectively). These data indicate that ram epididymides could be stored at 4º C for 48 h when epididymal spermatozoa cannot be immmediately collected and cryopreserved. These storage conditions might be possible to use for epididymal sperm recovery in wild ruminants.

show abstract

Section: Resultsmentioning

confidence: 99%

Characteristic of Frozen-Thawed Epididymal Spermatozoa and Refrigerated Storage of Ram Spermatozoa

Karja¹,

Respaty²,

Nuraini³

et al. 2010

J. Indonesian Trop. Anim. Agric.

View full text Add to dashboard Cite

show abstract

“…Spermatozoa from teratospermic males, especially the integrity of acrosomal membranes, are particularly sensitive to the steps of cryopreservation [28,29]. Despite the high proportion of abnormal spermatozoa produced in cheetah ejaculates, there is interest by captive managers in using artiWcial insemination (AI), preferably with frozenthawed spermatozoa, as a means of helping manage the ex situ population.…”

mentioning

confidence: 99%

“…During glycerol removal, the cell swells as it is exposed to near isotonic conditions and as extracellular water enters the cell faster than glycerol departs [24]. These volume excursions can disrupt both sperm membranes and the motility apparatus [29]. There also may be a toxic eVect [22], with protracted glycerol exposure adversely inXuencing sperm motility, membrane integrity, and overall survival [16].…”

mentioning

confidence: 99%

“…There are suYcient data demonstrating that ejaculates from teratospermic felids are particularly challenging to successfully cryopreserve [29]. In the teratospermic domestic cat, for example, the acrosome is especially sensitive to cooling, osmotic stressors, and the removal of cryoprotectant from the sperm suspension [27][28][29]. Therefore, the objectives of the present study were to conduct a thorough assessment of semen quality in wild-born cheetahs in Namibia while exploring the eYcacy of a two-step straw protocol for sperm cryopreservation.…”

mentioning

confidence: 99%

See 1 more Smart Citation

Cryopreservation of spermatozoa from wild-born Namibian cheetahs (Acinonyx jubatus) and influence of glycerol on cryosurvival

et al. 2006

Self Cite

View full text Add to dashboard Cite

“…Our laboratory has used certain felid species and genetic lineages to better understand the impact and etiology of teratospermia. Various studies have revealed that spermatozoa from teratospermic ejaculates demonstrate delayed capacitation [10], compromised acrosomal function [10], disrupted protein tyrosine phosphorylation [11,12], increased osmotic sensitivity [13,14], reduced zona penetration ability [15], and increased sensitivity to cooling [16] and cryopreservation [17]. These mechanisms no doubt contribute to the reduced fertilizing ability of teratospermic ejaculates in vitro, even after processing to isolate structurally normal spermatozoa for insemination [15].…”

Section: Introductionmentioning

confidence: 99%

Evidence for Compromised Metabolic Function and Limited Glucose Uptake in Spermatozoa from the Teratospermic Domestic Cat (Felis catus) and Cheetah (Acinonyx jubatus)1

Terrell

Wildt

Anthony

et al. 2010

Biology of Reproduction

View full text Add to dashboard Cite

Cheetahs and certain other felids consistently ejaculate high proportions (!60%) of malformed spermatozoa, a condition known as teratospermia, which is prevalent in humans. Even seemingly normal spermatozoa from domestic cat teratospermic ejaculates have reduced fertilizing capacity. To understand the role of sperm metabolism in this phenomenon, we conducted a comparative study in the normospermic domestic cat versus the teratospermic cat and cheetah with the general hypothesis that sperm metabolic function is impaired in males producing predominantly pleiomorphic spermatozoa. Washed ejaculates were incubated in chemically defined medium containing glucose and pyruvate. Uptake of glucose and pyruvate and production of lactate were assessed using enzyme-linked fluorescence assays. Spermatozoa from domestic cats and cheetahs exhibited similar metabolic profiles, with minimal glucose metabolism and approximately equimolar rates of pyruvate uptake and lactate production. Compared to normospermic counterparts, pyruvate and lactate metabolism were reduced in teratospermic cat and cheetah ejaculates, even when controlling for sperm motility. Rates of pyruvate and lactate (but not glucose) metabolism were correlated positively with sperm motility, acrosomal integrity, and normal morphology. Collectively, our findings reveal that pyruvate uptake and lactate production are reliable, quantitative indicators of sperm quality in these two felid species and that metabolic function is impaired in teratospermic ejaculates. Furthermore, patterns of substrate utilization are conserved between these species, including the unexpected lack of exogenous glucose metabolism. Because glycolysis is required to support sperm motility and capacitation in certain other mammals (including dogs), the activity of this pathway in felid spermatozoa is a target for future investigation.

show abstract

Osmotic properties of spermatozoa from felids producing different proportions of pleiomorphisms: influence of adding and removing cryoprotectant

Cited by 43 publications

References 41 publications

Characteristic of Frozen-Thawed Epididymal Spermatozoa and Refrigerated Storage of Ram Spermatozoa

Characteristic of Frozen-Thawed Epididymal Spermatozoa and Refrigerated Storage of Ram Spermatozoa

Cryopreservation of spermatozoa from wild-born Namibian cheetahs (Acinonyx jubatus) and influence of glycerol on cryosurvival

Evidence for Compromised Metabolic Function and Limited Glucose Uptake in Spermatozoa from the Teratospermic Domestic Cat (Felis catus) and Cheetah (Acinonyx jubatus)1

Contact Info

Product

Resources

About