Characteristic of Frozen-Thawed Epididymal Spermatozoa and Refrigerated Storage of Ram Spermatozoa

Karja, Ni Wayan Kurniani; Respaty, E.M.A; Nuraini, Indah; Prihatno, Surya Agus; Gustari, Sri

doi:10.14710/jitaa.35.1.63-67

Cited by 4 publications

(2 citation statements)

References 16 publications

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“…The significant decrease in motility, sperm concentration, viability, and intact acrosome of epididymal spermatozoa recovered post-mortem and maintained under similar conditions have been reported in mice [27], ram [10,28], boar [5], bull [1], stallion [29], and camel [30]. Variation in quality of cauda epididymal sperm cells recovered post-mortem has been ascribed to handling conditions or species differences [31,32].…”

Section: Resultssupporting

confidence: 56%

Evaluation of sperm recovered after slaughter from cauda epididymides of red Sokoto bucks

Abu¹,

Kisani²,

Ahemen³

2016

Vet World

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Aim:Viable spermatozoa could be recovered from the cauda epididymides for the purpose of preservation of genetic material of male animals with desirable traits and for use in reproductive biotechnology. The aim of this study was to determine the effect of storage time on testicular and epididymal biometry, sperm reserves and epididymal sperm characteristics of red Sokoto bucks post mortem.Materials and Methods:Testes-epididymides were collected immediately after slaughter of mature red Sokoto bucks and transported in ice chest to the laboratory. The samples were either processed immediately or stored at 5°C in refrigerator for 24, 48 h and then processed. The testes and epididymides were measured and weighed. Sperm motility, concentration, livability, morphology, intact acrosome, and sperm reserves from different treatment groups including control were evaluated and means (±standard error of mean) were recorded.Results:There was no significant difference (p>0.05) in the testicular and epididymal dimensions determined between the means of the groups. Percent sperm motility and viability decreased significantly (p<0.05) after 24 h from 69.00±0.46 and 71.27±0.50% to 50.60±0.48 and 60.47±0.70% at 48 h, respectively. Significant decreases (p<0.05) in epididymal sperm concentration and intact acrosome from 2.86±0.08 and 92.87±0.39 at 0 to 24 h of storage, respectively, were observed.Conclusion:The results of this study suggest that spermatozoa recovered from the epididymides of red Sokoto bucks were viable after storage for up to 48 h. Furthermore, this finding offers some hope that epididymal sperm recovered post-mortem can be used in assisted reproductive technologies.

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Section: Resultssupporting

confidence: 56%

Evaluation of sperm recovered after slaughter from cauda epididymides of red Sokoto bucks

Abu¹,

Kisani²,

Ahemen³

2016

Vet World

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“…They can be retrieved differently depending on the species involved or epididymal size. The most used EPS retrieval methods include a) Slicing/mincing (Kaabi et al, 2003;Parra-Forero et al, 2015), b) Incision (Karja et al, 2010;Alvarez et al, 2012;Ahmed, 2019), c) Retrograde flushing via ductus deferens (resulting in less contamination) (Lorraine Leibfried-Rutledge et al, 1997;Bertol, 2016) and d) Floatation (in case of species with tiny testicles) (Bertol, 2016). Good pregnancy rates have been reported using EPS for artificial insemination in sheep (87.5%, 58.5%, and 55.0%) (Ehling et al, 2006;Rickard et al, 2014;Fernández Abella et al, 2015) and goats (61.2%) (Ocampo et al, 2021).…”

Section: Introductionmentioning

confidence: 99%

The impact of retrieval method and breed on the motility and kinematic parameters of fresh and post-thaw ram epididymal spermatozoa

Mujitaba,

Kútvölgyi,

Debnár

et al. 2024

AVet

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This study was conducted to develop ideal post-mortem gamete retrieval and conservation methods to establish a Hungarian ex-situ in vitro gene bank. Pairs of testes from German Mutton Merino (n = 7) and Hungarian Black Racka (n = 7) rams were collected at a slaughterhouse, transported to the laboratory and stored overnight (4−5 °C) before processing. Post mortem ram epididymal spermatozoa (REPS) were obtained from the cauda epididymidis by slice or incision methods. Fresh samples were extended to 200 × 106/mL cell concentration, filled into mini straws and equilibrated at 5 °C for 2 h. Freezing was performed manually in a Styrofoam box. The fresh and post-thaw total motility, progressive motility and kinematic parameters of REPS were assessed using the CASA technique. The collection method did not affect significantly the fresh and post-thaw motility and kinematic parameters. Merino had higher (P < 0.05) testicular weight. Racka had significantly better fresh and post-thaw linear movement but had statistically the same (P > 0.05) cryotolerance as Merino. In conclusion, both collection methods were found suitable for REPS retrieval. The REPS from Racka exhibited better linear movement values than those from the Merino breed. The cryotolerance of REPS of both breeds was comparable.

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Alternative Opportunities to Collect Semen and Sperm Cells for Ex Situ In Vitro Gene Conservation in Sheep

et al. 2022

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The semen of domestic mammals is conventionally collected with an artificial vagina (AV) for artificial insemination (AI) or for short- or long-term storage. However, the procedure has certain drawbacks: animal training is not feasible in extensive animal care systems nor among wild species, as the trained animals sometimes fail to mount. Hence, there is a need for alternative semen collection methods. Electroejaculation (EEJ) and epididymal sperm recovery (ESR) are the two effective alternatives to AV. However, in recent years, animal welfare campaigners have called for the ban, in certain EU countries, of EEJ due to its inhumane nature. In this review, alternative methods of sperm collection (by EEJ and ESR, their qualities, and their freezing techniques) are highlighted, as well as the effects of EEJ on pre-freeze and post-thaw ram sperm quality parameters and the animal welfare progress made in EEJ between the 20th and 21st centuries. Additionally, the techniques for enhancing post-thaw sperm quality prior to freezing and for the freezing of EEJ and ESR spermatozoa are explored. ESR and EEJ are reliable alternatives to AV on certain occasions. EEJ is ideal for semen collection in wild or untrained animals, breeding soundness examinations, collection outside of the breeding season, and culling. At the same time, ESR is ideal in cases of castration, accidental death of elite sire, or postmortem for gene conservation purposes or assisted reproductive technologies (ARTs) studies.

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Characteristic of Frozen-Thawed Epididymal Spermatozoa and Refrigerated Storage of Ram Spermatozoa

Cited by 4 publications

References 16 publications

Evaluation of sperm recovered after slaughter from cauda epididymides of red Sokoto bucks

Evaluation of sperm recovered after slaughter from cauda epididymides of red Sokoto bucks

The impact of retrieval method and breed on the motility and kinematic parameters of fresh and post-thaw ram epididymal spermatozoa

Alternative Opportunities to Collect Semen and Sperm Cells for Ex Situ In Vitro Gene Conservation in Sheep

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