Osmoregulated periplasmic glucans synthesis gene family of Shigella flexneri

Liu, Liu; Dharne, Mahesh; Porteen, K.; Smith, Allen; Meng, Jianghong; Fan, Mingtao; Boren, Tara L.; Ranallo, Ryan T.; Bhagwat, Arvind A.

doi:10.1007/s00203-009-0538-z

Cited by 8 publications

(11 citation statements)

References 22 publications

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“…Lastly, we reasoned that although no OPG synthesis has been reported in media with osmolarity >420 mMos Mol -1 [5,6], there might be a low level of OPG synthesis below the detection limits. To this end, we monitored the transcript levels of opgG and opgH genes at various osmolarity (Figure 4).…”

Section: Transcription Levels Of Opggh Genes As a Function Of Medium mentioning

confidence: 98%

“…Under optimal swarm conditions, the OPG mutant and wild-type strains exhibited indistinguishable swim phenotypes with swim radii of 51+2.5 mm and 54+4.5 mm, respectively. Since no OPG synthesis has ever been reported in growth media with an osmolarity >420 mMos Mol -1 [1,[3][4][5][6]25], the non-swarming phenotype of opgGH mutant appeared unexpected and thus, was investigated further.…”

Section: Swarm and Swim Motility Phenotypes Of Opggh Mutant And Mediumentioning

confidence: 99%

“…Large quantities of OPG are synthesized as the osmolarity of the surrounding medium decreases [3]. In Escherichia coli, Salmonella enterica serovar Typhimurium and Shigella flexneri, OPG synthesis is catalyzed by the products of opgG and opgH genes, which are transcribed as a single operon [2,[4][5][6]. Studies in several microorganisms, including plant and animal pathogens as well as plant symbionts, have shown the importance of OPG in successful host invasion [6][7][8].…”

Section: Introductionmentioning

confidence: 99%

“…However, a role for OPG in swarm motility has not been investigated. Given the fact that virtually no OPG synthesis is reported when bacteria are grown on media with osmolarity >400 mMos Mol -1 [3][4][5][6], the requirement of OPG for swarm motility of S. enterica serovar Typhimurium appears paradoxical. Most swarm media reported for Salmonella have osmolarity >400 mMos Mol -1 [15,16].…”

Section: Introductionmentioning

confidence: 99%

“…It has been documented in E. coli, S. flexneri and Salmonella sp. that synthesis of OPG is inversely proportional to the medium osmolarity, with maximum OPG synthesis occurring around 95 mMos Mol -1 (i.e., LB broth without NaCl) [3][4][5][6]. In order to examine the alleged role of OPG in swarming motility, we monitored opgGH transcripts from cells grown in liquid and swarm growth media of varying osmolarities.…”

Section: Introductionmentioning

confidence: 99%

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Swarm and swim motilities of Salmonella enterica serovar Typhimurium and role of osmoregulated periplasmic glucans

Dharne¹,

Porteen²,

Murphy³

et al. 2015

Microbiol Discov

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Section: Transcription Levels Of Opggh Genes As a Function Of Medium mentioning

confidence: 98%

Section: Swarm and Swim Motility Phenotypes Of Opggh Mutant And Mediumentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 3 more Smart Citations

Swarm and swim motilities of Salmonella enterica serovar Typhimurium and role of osmoregulated periplasmic glucans

Dharne¹,

Porteen²,

Murphy³

et al. 2015

Microbiol Discov

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Role of anionic charges of osmoregulated periplasmic glucans of Salmonella enterica serovar Typhimurium SL1344 in mice virulence

et al. 2012

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opgB gene of Salmonella enterica serovar Typhimurium was identified earlier in a genome-wide screen for mice virulence (Valentine et al. in Infect Immun 66:3378-3383, 1998). Although mutation in opgB resulted in avirulent Salmonella strain, how this gene contributes to pathogenesis remains unclear. Based on DNA homology, opgB is predicted to be responsible for adding phosphoglycerate residues to osmoregulated periplasmic glucans (OPGs) giving them anionic characteristics. In Escherichia coli, yet another gene, opgC, is also reported to contribute to anionic characteristics of OPGs by adding succinic acid residues. We constructed opgB, opgC, and opgBC double mutants of S. enterica serovar Typhimurium strain SL1344. As predicted opgBC mutant synthesized neutral OPGs that were devoid of any anionic substituents. However, opgB, opgC, and opgBC mutations had no significant impact on mice virulence as well as on competitive organ colonization. In low osmotic conditions, opgB, opgC, and opgBC mutants exhibited delay in growth initiation in the presence of sodium deoxycholate. Anionic substituents of OPGs from Salmonella although appear to be needed to overcome resistance of deoxycholate in hypoosmotic growth media, no evidence was found for their role in mice virulence.

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Transcriptomic Analysis of the Swarm Motility Phenotype of Salmonella enterica Serovar Typhimurium Mutant Defective in Periplasmic Glucan Synthesis

Bhagwat¹,

Young

Smith

et al. 2017

Curr Microbiol

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Movement of food-borne pathogens on moist surfaces enables them to migrate towards more favorable niches and facilitate their survival for extended periods of time. Salmonella enterica serovar Typhimurium mutants defective in Osmoregulated periplasmic glucans (OPG) synthesis are unable to exhibit motility on moist surfaces (swarming); however, their mobility in liquid (swim motility) remains unaffected. In order to understand the role of OPG in swarm motility, transcriptomic analysis was performed using cells growing on a moist agar surface. In opgGH deletion mutant, lack of OPG significantly altered transcription of 1039 genes out of total 4712 genes (22%). Introduction of a plasmid-borne copy of opgGH into opgGH deletion mutant restored normal expression of all but 30 genes, indicating a wide-range influence of OPG on gene expression under swarm motility condition. Major pathways that were differentially expressed in opgGH mutants were motility, virulence and invasion, and genes related to the secondary messenger molecule, cyclic di-GMP. These observations provide insights and help explain the pleiotropic nature of OPG mutants such as sub-optimal virulence and competitive organ colonization in mice, biofilm formation, and sensitivity towards detergent stress.

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Osmoregulated periplasmic glucans synthesis gene family of Shigella flexneri

Abstract: The online version of this article (doi:10.1007/s00203-009-0538-z) contains supplementary material, which is available to authorized users.

Cited by 8 publications

References 22 publications

Swarm and swim motilities of Salmonella enterica serovar Typhimurium and role of osmoregulated periplasmic glucans

Swarm and swim motilities of Salmonella enterica serovar Typhimurium and role of osmoregulated periplasmic glucans

Role of anionic charges of osmoregulated periplasmic glucans of Salmonella enterica serovar Typhimurium SL1344 in mice virulence

Transcriptomic Analysis of the Swarm Motility Phenotype of Salmonella enterica Serovar Typhimurium Mutant Defective in Periplasmic Glucan Synthesis

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