Osmolytes Accumulation, Cell Membrane Integrity, and Antioxidant Enzymes in Sugarcane Varieties Differing in Salinity Tolerance

Adaptation of sugarcane plants to saline soil

“…A higher increase of proline and GB and lower lipid peroxidation in CoM-265 (salt tolerant) sugarcane cultivar, compared to cv. CoC-671 (salt susceptible), was found by Satbhai and Naik (2014) IAC 87-3396 was greater than in cv. SP 81-3250 by 35%.…”

“…Poonsawat et al (2015) verified that genes like sugarcane shaggy-like kinase (SuSK), sucrose transporter (SUT), proline biosynthesis (pyrolline-5-carboxylate synthetase; P5CS), Na + /H + exchanger for ion homeostasis (NHX1), and catalase (CAT2) showed higher expression in sugarcane stressed by salinity, contributing to tolerance of those plants by Na + reduction, soluble sugars enrichment and increase of free proline. Satbhai and Naik (2014) showed that tolerance to salinity in different sugarcane cultivars was related to better ability for ROS scavenging, maintaining low lipid peroxidation and higher membrane stability, as well as compatible osmolytes. The ability of plants to produce antioxidants enzymes under stress is a well-known mechanism of tolerance.…”

Elevated Levels of Osmoprotectants Securing Sugarcane (Saccharum officinarum L.) during Salinity Stress

“…Earlier it was reported that accumulation of osmolyte like proline is known to occur under osmotic stress (Hasegawa et al, 2000;Garg et al, 2002;Munns, 2005). The levels of free proline, an important osmolyte and free radical scavenger increased substantially in salt tolerant (CoM-0265) and salt susceptible (CoC-671) sugarcane varieties due to salinity (Satbhai et al, 2014). It was further noticed that the under unstressed (control) condition the level of proline was 0.486 in CoM-265 and it increased to 1.844 umol g -1 FW an increase of 279 % over control.…”

“…Glycine betaine in Sugarcane variety MS-10001 was 2.22 umoles g -1 FW at 0 dSm -1 (control), 3.04 umoles g -1 FW at 2 dSm -1 , 3.87 umoles g -1 FW at 4 dSm -1 , 5.87 umoles g -1 FW at 8 dSm -1 , whereas, in CoC 671 it was 1.79 umoles g -1 FW at 0 dSm -1 (control), 3 umoles g -1 FW at 2 dSm -1 , 3.72 umoles g -1 FW at 4 dSm -1 , 5.31 umoles g -1 FW at 8 dSm -1 . Satbhai et al, (2014) reported that in salt tolerant (CoM-265) and salt susceptible (CoC-671) varieties of sugarcane, the levels of glycine-betaine were increased significantly due to salt stress. In CoM 265 the level increased from 28.23 to 173.6 ug g -1 FW, an increase of 515 %, as against with an increase of 51 % in CoC-671, a salt susceptible variety.…”

Differential Proteomic Analysis Reveals the Effect of Calcium on Malus baccata Borkh. Leaves under Temperature Stress

“…Membrane integrity was measured according to the method of Satbhai and Naik [ 84 ]. Ten leaf discs (0.1 cm −2 ) were thoroughly rinsed three times with deionized water for 1 min each time, and immersed together in 10 mL deionized water at 40 °C for 30 min.…”