Thirty days grown seedlings of eight genotypes of groundnut viz. RHRG-6083, TAG-24, TG-60(LC), Karad-4-11, SB-XI, RHRG-6097, RHRG-6021, and RHRG-6055 were subjected to moisture stress by withholding irrigation for 15 days in pot culture experiment in order to evaluate its effect of on RWC , proline accumulation, soluble proteins, chlorophyll content and activities of superoxide dismutase (SOD), peroxidase (POX), glutathione reductase (GR) and lipid peroxidation (MDA).The levels of proline, soluble protein and the activity of all four antioxidative enzymes were found to be increased with MDA content in all genotypes of groundnut during water stress, however the chlorophyll and RWC content were found to be decreased. This study revealed that under water stress condition SB-XI, TAG-24 and RHRG-6021 showed higher increase of proline content and activities of antioxidative enzymes with lower reduction in RWC and chlorophyll. These three genotypes are stable during stress and seems to be promising for drought tolerance.

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The soybean seed is highly susceptible to field weathering and mechanical damage which adversely affect its longevity. Mechanical injury can occur at any time during harvesting, drying and storage conditioning of seeds. The seed coat color and leachate conductivity of soybean has been correlated with seed longevity and black seed coat color has been reported to be positively correlated with better seed longevity. In order to understand the physico-chemical attributes related to soybean seed longevity, biochemical and molecular analysis of the parents exhibiting black (Birsasoya-1) and yellow seed coat colour (EC 241780) and the eleven F3 progenies of the cross exhibiting brown, yellow and black seed coat colour was carried out. The results revealed that vita-E, lignin, calcium content and activity of antioxidative enzymes appeared to be positively correlated with soybean seed longevity and levels were higher in black and brown seed coat color progenies. The lipid peroxidation rate was inversely related to membrane injury caused by ROS and comparatively much less lipid peroxidation rate was recorded in black and brown seed coat colour parents and progenies having better seed longevity. The SSR primers Satt162, Satt523 and Satt453 which are either linked with seed coat colour and seed permeability exhibited a specific size allelic fragments in soybean genotypes and crosses with better seed longevity.

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