Osmolality and composition of the extender during the cold storage of Prochilodus lineatus (Characiformes: Prochilodontidae) sperm

Viveiros, Ana Tereza de Mendonça; Taffarel, Taís Regina; Leal, Marcelo de Castro

doi:10.1590/1982-0224-20130207

Cited by 12 publications

(9 citation statements)

References 16 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Semen analysis using CASA has been reported in other species of fish and has been shown to be a good tool to help determine sperm quality; it provides more information than subjective analysis and more accurate results (NYNCA et al, 2014;VIVEIROS et al, 2014;JUDYCKA et al, 2015;MARIA et al, 2015). This is one of the first studies using CASA in the analysis of fresh and cryopreserved sperm for P. brevis.…”

Section: Sperm Cryopreservation and Thawingmentioning

confidence: 95%

Cryopreservation of Prochilodus brevis semen: freezing media and thawing rates

Nunes

Oliveira

Lopes

et al. 2016

SCA

View full text Add to dashboard Cite

Prochilodus brevis is a migratory fish, an important component of its river ecosystem and an appreciated animal in northeastern cuisine. However, human activities have threatened its survival. Thus, researchers have become interested in developing genetic material storage protocols, such as seminal cryopreservation. Therefore, determination of the appropriate freezing media and thawing rate is a fundamental step toward the use of this biotechnology in the production of common curimatã and for reducing risks to the species' survival. As such, the aim of the current study was to evaluate the effect of different freezing media and thawing rates on the quality of cryopreserved semen from P. brevis. For this study, males received a single dose of pituitary extract of carp 18 hours before semen collection. The semen samples were diluted in 5% glucose + 10% methyl glycol (MG), 5% glucose + 10% dimethyl sulfoxide (DMSO), 0.9% NaCl + 10% methyl glycol, and 0.9% NaCl + 10% dimethyl sulfoxide, loaded into 0.25-mL straws and frozen in liquid nitrogen vapor. The semen from each treatment was thawed at three different thawing rates: 25 °C for 30 sec, 30 °C for 16 sec and 40 °C for 12 sec. Motility, vitality and morphology analyses were performed by computer-assisted sperm analysis (CASA). The characteristics of the fresh sperm mostly resembled those found in the literature. For the parameters analyzed, fresh sperm presented higher sperm quality in comparison to all treatments with cryopreserved sperm (p<0.05), except for the characteristic of normal morphology, for which the sperm cryopreserved in glucose and MG did not differ statistically from the fresh sperm. For the cryopreserved semen, the greatest results of total motility and curvilinear velocity (VCL) were obtained using glucose and DMSO, regardless of the thawing rate employed. For the straight-line velocity (VSL) and average path velocity (VAP), DMSO showed the best results, regardless of the diluent and thawing rate. With regard to vitality, the highest values were achieved when DMSO and thawing rates of 30 °C for 16 sec or 40 °C for 12 sec were used. In the morphological analysis, the greatest percentage of normal sperm cells was obtained using thawing rates of 25 °C for 30 sec and 40 °C for 12 sec, regardless of the freezing media. Sperm quality was found to suffer interference from the freezing media, as well as from interaction between its components (diluent and cryoprotectant) and the thawing rate used. Under the methodological conditions employed, the use of 5% glucose + 10% DMSO and a thawing rate of 30 °C for 16 seconds or 40 °C for 12 seconds is recommended for P. brevis semen cryopreservation. ResumoO Prochilodus brevis é um peixe reofílico, importante componente do ecossistema fluvial e apreciado na culinária nordestina. No entanto, ações antrópicas têm ameaçado sua sobrevivência. Desta forma, surge, nos pesquisadores, o interesse no desenvolvimento de protocolos de conservação do material genético, como a criopreservação seminal. Logo, a deter...

show abstract

Section: Sperm Cryopreservation and Thawingmentioning

confidence: 95%

Cryopreservation of Prochilodus brevis semen: freezing media and thawing rates

Nunes

Oliveira

Lopes

et al. 2016

SCA

View full text Add to dashboard Cite

show abstract

“…The extender solution powdered coconut water ACP (ACP Biotecnologia, Fortaleza, CE, Brazil), was developed for several freshwater species; this solution has recently been adapted for semen fish, received the name ACP-104 (Viveiros et al, 2010), and was tested in Prochilodus lineatus (Viveiros et al, 2014b).…”

Section: Diferentes Soluções Diluidoras Para O Resfriamento Do Sêmen mentioning

confidence: 99%

Different extenders solutions for tambaqui semen cooling

Garcia

Vasconcelos

Povh

et al. 2016

Pesq. agropec. bras.

View full text Add to dashboard Cite

-The objective of this work was to evaluate the efficiency of extenders solutions in tambaqui (Colossoma macropomum) semen cooling at 6°C. The treatments consisted of semen dilutions with three extenders -BTS, HBSS, and ACP-104 -, and a control with undiluted semen. Cooled semen was evaluated by 24, 48, 72, 96, and 120 hours. There were differences for the duration of motility and fertilization rates among the semen dilutions. Motility duration proved a highly variable evaluation after 72 hours. Dilution testing can be performed by the three products, and cooling storage for 72 hours is most effective with BTS extender.Index terms: Colossoma macropomum, ACP, BTS, HBSS, motility, sperm. Diferentes soluções diluidoras para o resfriamento do sêmen de tambaquiResumo -O objetivo deste trabalho foi avaliar a eficiência de soluções diluidoras no resfriamento do sêmen de tambaqui (Colossoma macropomum) a 6°C. Os tratamentos consistiram de diluições de amostras de sêmen com três soluções -BTS, HBSS e ACP-104, e de um controle com sêmen não diluído. O sêmen resfriado foi avaliado por 24, 48, 72, 96 e 120 horas. Houve diferenças quanto à duração da motilidade e taxa de fertilização entre as diluições do sêmen. A duração da motilidade mostrou-se altamente variável depois de 72 horas. Os testes de diluição podem ser realizados pelos três diluidores, e seu armazenamento por 72 horas é mais eficaz com o diluidor BTS.

show abstract

“…Wilson‐Leedy and Ingermann () observed 90 ± 6% motility, 104 ± 9 μm/s curvilinear velocity, 77 ± 15 μm/s average path velocity, 66 ± 17 μm/s straight line velocity and 74 ± 10% straightness for fresh D. rerio sperm. Viveiros et al () observed 75 ± 12% motility, 176 ± 48 μm/s curvilinear velocity, 97 ± 37 μm/s straight line velocity, 144 ± 51 μm/s average path velocity for fresh P. lineatus sperm using the Sperm Class Analyzer™ software (SCA™ 2010, Microptics, S.L. Version 5.1).…”

Section: Discussionmentioning

confidence: 99%

“…Fish semen cryopreservation technology has expanded considerably worldwide with the use of portable nitrogen vapour cylinders, known as “dry‐shippers.” Semen is packaged in straws, added to the cylinder and subjected to uncontrolled speeds between 30 and 40°C/min. Fish semen from neotropical Characiformes and Siluriformes orders has been successfully frozen using this technique (Viveiros, Taffarel, & Leal, ).…”

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Effects of cooling rates on the quality of Prochilodus lineatus (Valenciennes, 1836) sperm

Paula

Murgas

Castro

et al. 2019

Reprod Domestic Animals

View full text Add to dashboard Cite

This study aims to investigate the effect of different cooling rates on the semen cryopreservation of curimba (Prochilodus lineatus). Nineteen ejaculates were obtained from adults males and cryopreserved at 15°C/min (CR15), 30°C/min (CR30) (controlled temperature inside and outside straw, speed was stable during freezing) and direct freezing in liquid nitrogen vapour (~35.6°C/min) (CRNV). The straws were thawed and seminal parameters evaluated. DNA fragmentation through the comet assay was assessed. A fresh sperm sample was not frozen and used for analyses. Data were submitted to an analysis of variance (ANOVA), and means were compared by Scott–Knott test (p < 0.05) using the R Software. Mean motility percentage was 100%, and motility duration was 39.5 ± 5.7 s for the fresh sperm (subjective analysis); 58.9 ± 8.0% and 24.5 ± 5.7 s for CR15; 64.8 ± 4.8% and 26.5 ± 7.1 s for CR30; and 50.1 ± 16% and 25.7 ± 4.7 s for CRNV, respectively. Motility percentages were higher and equal between CR15 and CR30 compared to CRNV (p < 0.05). Some sperm motion kinetics, namely average path velocity (VAP) and straight line velocity (VAS), were higher for CR30 (p < 0.05), while curvilinear velocity (VCL) and velocity progression (PRO) were lower for CRNV (p < 0.05). Straightness (STR) and wobble (WOB) were the same among treatments (p > 0.05). Sperm morphology results indicated higher means for total morphological sperm alterations in CRNV. All cooling rates caused sperm DNA fragmentation, although CR30 provided a less harmful effect. This is the first report for cryopreserved P. lineatus sperm preserved under different controlled cooling rates. The cooling rate of 30°C/min is indicated for the cryopreservation of this fish sperm as it led to the lowest detrimental spermatozoa effects.

show abstract

Osmolality and composition of the extender during the cold storage of Prochilodus lineatus (Characiformes: Prochilodontidae) sperm

Cited by 12 publications

References 16 publications

Cryopreservation of Prochilodus brevis semen: freezing media and thawing rates

Cryopreservation of Prochilodus brevis semen: freezing media and thawing rates

Different extenders solutions for tambaqui semen cooling

Effects of cooling rates on the quality of Prochilodus lineatus (Valenciennes, 1836) sperm

Contact Info

Product

Resources

About