OsDOG1L-3 regulates seed dormancy through the abscisic acid pathway in rice

Wang, Qian; Lin, Qian; Wu, Tao; Duan, Erchao; Huang, Yunshuai; Yang, Chen; Mou, Changling; Lan, Jie; Zhou, Chunlei; Xie, Kun; Liu, Xi; Zhang, Xin; Guo, Xiuping; Wang, Jie; Jiang, Ling; Wan, Jianmin

doi:10.1016/j.plantsci.2020.110570

Cited by 26 publications

(20 citation statements)

References 56 publications

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“…This subsequently increases the content of endogenous JA and represses seed germination [49]. Furthermore, OsbZIP75 directly binds to the promoter of DELAY OF GERMINATION 1 (DOG1) and promotes the accumulation of OsDOG1L-3 and inhibits seed germination [50]. However, all the above-mentioned OsbZIPs negatively regulate rice germination.…”

Section: Discussionmentioning

confidence: 99%

Genome-Wide Identification and Expression Analysis of OsbZIP09 Target Genes in Rice Reveal Its Mechanism of Controlling Seed Germination

Zhu¹,

Wang²,

Lu³

et al. 2021

IJMS

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Seed dormancy and germination are key events in plant development and are critical for crop production, and defects in seed germination or the inappropriate release of seed dormancy cause substantial losses in crop yields. Rice is the staple food for more than half of the world’s population, and preharvest sprouting (PHS) is one of the most severe problems in rice production, due to a low level of seed dormancy, especially under warm and damp conditions. Therefore, PHS leads to yield loss and a decrease in rice quality and vitality. We reveal that mutation of OsbZIP09 inhibited rice PHS. Analysis of the expression of OsbZIP09 and its encoded protein sequence and structure indicated that OsbZIP09 is a typical bZIP transcription factor that contains conserved bZIP domains, and its expression is induced by ABA. Moreover, RNA sequencing (RNA-seq) and DNA affinity purification sequencing (DAP-seq) analyses were performed and 52 key direct targets of OsbZIP09 were identified, including OsLOX2 and Late Embryogenesis Abundant (LEA) family genes, which are involved in controlling seed germination. Most of these key targets showed consistent changes in expression in response to abscisic acid (ABA) treatment and OsbZIP09 mutation. The data characterize a number of key target genes that are directly regulated by OsbZIP09 and contribute to revealing the molecular mechanism that underlies how OsbZIP09 controls rice seed germination.

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Section: Discussionmentioning

confidence: 99%

Genome-Wide Identification and Expression Analysis of OsbZIP09 Target Genes in Rice Reveal Its Mechanism of Controlling Seed Germination

Zhu¹,

Wang²,

Lu³

et al. 2021

IJMS

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“…In addition, the rice homolog of Arabidopsis DOG1, OsDOG1L-3, is responsible for the function of the QTL qsd-1-1, which has a large effect on seed dormancy in rice. Expression of OsDOG1L-3 enhanced seed dormancy and was enhanced by ABA [4] .…”

Section: Introductionmentioning

confidence: 98%

“…These genes include PHS9, which regulates PHS by adjusting the ABA sensitivity [5] ; OsMFT2, an ABA signaling gene that regulates the sensitivity to ABA by interacting with three OsbZIP transcription factors [6] ; and OsDOG1L-3, a gene that is induced by ABA and positively regulates ABA pathways. Overexpression of OsDOG1L-3 results in enhanced seed dormancy [4] . Recent studies have provided evidence that GA participates in seed dormancy through the action of the microRNA miR156, which is an important regulator of GA pathways [1] .…”

Section: Introductionmentioning

confidence: 99%

“…Sensitivity to abscisic acid (ABA) has been frequently found to correlate with seed dormancy and to further affect PHS tolerance [2,3] . ABA content was originally thought to have little effect on seed dormancy, but the results of recent studies have shown that ABA metabolism may play a part in dormancy release in after-ripened grains [2,4,5,6] . Changes in ABA content have been found to correlate with the timing of germination of imbibed dormant seeds [2,4] .…”

Section: Introductionmentioning

confidence: 99%

“…ABA content was originally thought to have little effect on seed dormancy, but the results of recent studies have shown that ABA metabolism may play a part in dormancy release in after-ripened grains [2,4,5,6] . Changes in ABA content have been found to correlate with the timing of germination of imbibed dormant seeds [2,4] . Gibberellic acid (GA) was found to have an effect on promoting germination, and there is evidence that a high concentration of GA can release seeds from dormancy.…”

Section: Introductionmentioning

confidence: 99%

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A Comparative Transcriptome Analysis Reveals New Insights into Pre-Harvest Sprouting (PHS) in Wheat

Qiao

Wang

et al. 2021

Preprint

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Background: pre-harvest sprouting (PHS) is a significant cause of yield loss in cereal crops, and is an important topic of study for the improvement of wheat quality. Many studies have focused on PHS in wheat during the last 10 years, especially on the involvement of abscisic acid (ABA) in PHS, however, a lot remained unknown about this topic.Results: In this study, a PHS resistant line was isolated from an ethylmethane sulfonate (EMS) mutant population derived from the wheat cultivar ‘Long 13-3778’, namely ‘LQ18’. The mutant line LQ18 showed highly significant resistance to PHS compared with the wild-type. Transcriptome sequencing was conducted to determine the differences between the LQ18 mutant and the wild-type at the level of gene expression. The results showed no conclusive evidence that the ABA biosynthesis and signaling pathways contribute to the differences in PHS between the mutant and the wild-type, and some genes and their alleles associated with PHS tolerance showed differential expression between the mutant and wild-type lines. The most interesting result of this study was that the expression levels of the chitinase family genes showed significant differences between the mutant and the wild-type as determined by GO enrichment analysis, and a subsequent analysis of differential expression profiling of the chitinase genes led to the same conclusion.Conclusions: Transcriptomic analysis in this study have revealed the global transcriptome profiles of the PHS sensitive wheat cultivar ‘Long 13-3778’ and its PHS resistant mutants. Furthermore, this study has proposed a possible explanation of the connection between PHS and the chitinase family for the first time, which added to our understanding of PHS and seed dormancy in common wheat.

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A rice seed-specific glycine-rich protein OsDOR1 interacts with GID1 to repress GA signaling and regulates seed dormancy

et al. 2023

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OsDOG1L-3 regulates seed dormancy through the abscisic acid pathway in rice

Cited by 26 publications

References 56 publications

Genome-Wide Identification and Expression Analysis of OsbZIP09 Target Genes in Rice Reveal Its Mechanism of Controlling Seed Germination

Genome-Wide Identification and Expression Analysis of OsbZIP09 Target Genes in Rice Reveal Its Mechanism of Controlling Seed Germination

A Comparative Transcriptome Analysis Reveals New Insights into Pre-Harvest Sprouting (PHS) in Wheat

A rice seed-specific glycine-rich protein OsDOR1 interacts with GID1 to repress GA signaling and regulates seed dormancy

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